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Proteomic Analysis of the Schistosoma mansoni Miracidium

Despite extensive control efforts, schistosomiasis continues to be a major public health problem in developing nations in the tropics and sub-tropics. The miracidium, along with the cercaria, both of which are water-borne and free-living, are the only two stages in the life-cycle of Schistosoma mans...

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Autores principales: Wang, Tianfang, Zhao, Min, Rotgans, Bronwyn A., Strong, April, Liang, Di, Ni, Guoying, Limpanont, Yanin, Ramasoota, Pongrama, McManus, Donald P., Cummins, Scott F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723143/
https://www.ncbi.nlm.nih.gov/pubmed/26799066
http://dx.doi.org/10.1371/journal.pone.0147247
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author Wang, Tianfang
Zhao, Min
Rotgans, Bronwyn A.
Strong, April
Liang, Di
Ni, Guoying
Limpanont, Yanin
Ramasoota, Pongrama
McManus, Donald P.
Cummins, Scott F.
author_facet Wang, Tianfang
Zhao, Min
Rotgans, Bronwyn A.
Strong, April
Liang, Di
Ni, Guoying
Limpanont, Yanin
Ramasoota, Pongrama
McManus, Donald P.
Cummins, Scott F.
author_sort Wang, Tianfang
collection PubMed
description Despite extensive control efforts, schistosomiasis continues to be a major public health problem in developing nations in the tropics and sub-tropics. The miracidium, along with the cercaria, both of which are water-borne and free-living, are the only two stages in the life-cycle of Schistosoma mansoni which are involved in host invasion. Miracidia penetrate intermediate host snails and develop into sporocysts, which lead to cercariae that can infect humans. Infection of the snail host by the miracidium represents an ideal point at which to interrupt the parasite’s life-cycle. This research focuses on an analysis of the miracidium proteome, including those proteins that are secreted. We have identified a repertoire of proteins in the S. mansoni miracidium at 2 hours post-hatch, including proteases, venom allergen-like proteins, receptors and HSP70, which might play roles in snail-parasite interplay. Proteins involved in energy production and conservation were prevalent, as were proteins predicted to be associated with defence. This study also provides a strong foundation for further understanding the roles that neurohormones play in host-seeking by schistosomes, with the potential for development of novel anthelmintics that interfere with its various life-cycle stages.
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spelling pubmed-47231432016-01-30 Proteomic Analysis of the Schistosoma mansoni Miracidium Wang, Tianfang Zhao, Min Rotgans, Bronwyn A. Strong, April Liang, Di Ni, Guoying Limpanont, Yanin Ramasoota, Pongrama McManus, Donald P. Cummins, Scott F. PLoS One Research Article Despite extensive control efforts, schistosomiasis continues to be a major public health problem in developing nations in the tropics and sub-tropics. The miracidium, along with the cercaria, both of which are water-borne and free-living, are the only two stages in the life-cycle of Schistosoma mansoni which are involved in host invasion. Miracidia penetrate intermediate host snails and develop into sporocysts, which lead to cercariae that can infect humans. Infection of the snail host by the miracidium represents an ideal point at which to interrupt the parasite’s life-cycle. This research focuses on an analysis of the miracidium proteome, including those proteins that are secreted. We have identified a repertoire of proteins in the S. mansoni miracidium at 2 hours post-hatch, including proteases, venom allergen-like proteins, receptors and HSP70, which might play roles in snail-parasite interplay. Proteins involved in energy production and conservation were prevalent, as were proteins predicted to be associated with defence. This study also provides a strong foundation for further understanding the roles that neurohormones play in host-seeking by schistosomes, with the potential for development of novel anthelmintics that interfere with its various life-cycle stages. Public Library of Science 2016-01-22 /pmc/articles/PMC4723143/ /pubmed/26799066 http://dx.doi.org/10.1371/journal.pone.0147247 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Tianfang
Zhao, Min
Rotgans, Bronwyn A.
Strong, April
Liang, Di
Ni, Guoying
Limpanont, Yanin
Ramasoota, Pongrama
McManus, Donald P.
Cummins, Scott F.
Proteomic Analysis of the Schistosoma mansoni Miracidium
title Proteomic Analysis of the Schistosoma mansoni Miracidium
title_full Proteomic Analysis of the Schistosoma mansoni Miracidium
title_fullStr Proteomic Analysis of the Schistosoma mansoni Miracidium
title_full_unstemmed Proteomic Analysis of the Schistosoma mansoni Miracidium
title_short Proteomic Analysis of the Schistosoma mansoni Miracidium
title_sort proteomic analysis of the schistosoma mansoni miracidium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723143/
https://www.ncbi.nlm.nih.gov/pubmed/26799066
http://dx.doi.org/10.1371/journal.pone.0147247
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