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Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC
Neonatal Meningitis Escherichia coli (NMEC) is one of the most common causes of neonatal bacterial meningitis in the US and elsewhere resulting in mortality or neurologic deficits in survivors. Large plasmids have been shown experimentally to increase the virulence of NMEC in the rat model of neonat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723317/ https://www.ncbi.nlm.nih.gov/pubmed/26800268 http://dx.doi.org/10.1371/journal.pone.0147757 |
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author | Nicholson, Bryon A. West, Aaron C. Mangiamele, Paul Barbieri, Nicolle Wannemuehler, Yvonne Nolan, Lisa K. Logue, Catherine M. Li, Ganwu |
author_facet | Nicholson, Bryon A. West, Aaron C. Mangiamele, Paul Barbieri, Nicolle Wannemuehler, Yvonne Nolan, Lisa K. Logue, Catherine M. Li, Ganwu |
author_sort | Nicholson, Bryon A. |
collection | PubMed |
description | Neonatal Meningitis Escherichia coli (NMEC) is one of the most common causes of neonatal bacterial meningitis in the US and elsewhere resulting in mortality or neurologic deficits in survivors. Large plasmids have been shown experimentally to increase the virulence of NMEC in the rat model of neonatal meningitis. Here, 9 ExPEC-like plasmids were isolated from NMEC and sequenced to identify the core and accessory plasmid genes of ExPEC-like virulence plasmids in NMEC and create an expanded plasmid phylogeny. Results showed sequenced virulence plasmids carry a strongly conserved core of genes with predicted functions in five distinct categories including: virulence, metabolism, plasmid stability, mobile elements, and unknown genes. The major functions of virulence-associated and plasmid core genes serve to increase in vivo fitness by adding multiple iron uptake systems to the genetic repertoire to facilitate NMEC’s survival in the host’s low iron environment, and systems to enhance bacterial resistance to host innate immunity. Phylogenetic analysis based on these core plasmid genes showed that at least two lineages of ExPEC-like plasmids could be discerned. Further, virulence plasmids from Avian Pathogenic E. coli and NMEC plasmids could not be differentiated based solely on the genes of the core plasmid genome. |
format | Online Article Text |
id | pubmed-4723317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47233172016-01-30 Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC Nicholson, Bryon A. West, Aaron C. Mangiamele, Paul Barbieri, Nicolle Wannemuehler, Yvonne Nolan, Lisa K. Logue, Catherine M. Li, Ganwu PLoS One Research Article Neonatal Meningitis Escherichia coli (NMEC) is one of the most common causes of neonatal bacterial meningitis in the US and elsewhere resulting in mortality or neurologic deficits in survivors. Large plasmids have been shown experimentally to increase the virulence of NMEC in the rat model of neonatal meningitis. Here, 9 ExPEC-like plasmids were isolated from NMEC and sequenced to identify the core and accessory plasmid genes of ExPEC-like virulence plasmids in NMEC and create an expanded plasmid phylogeny. Results showed sequenced virulence plasmids carry a strongly conserved core of genes with predicted functions in five distinct categories including: virulence, metabolism, plasmid stability, mobile elements, and unknown genes. The major functions of virulence-associated and plasmid core genes serve to increase in vivo fitness by adding multiple iron uptake systems to the genetic repertoire to facilitate NMEC’s survival in the host’s low iron environment, and systems to enhance bacterial resistance to host innate immunity. Phylogenetic analysis based on these core plasmid genes showed that at least two lineages of ExPEC-like plasmids could be discerned. Further, virulence plasmids from Avian Pathogenic E. coli and NMEC plasmids could not be differentiated based solely on the genes of the core plasmid genome. Public Library of Science 2016-01-22 /pmc/articles/PMC4723317/ /pubmed/26800268 http://dx.doi.org/10.1371/journal.pone.0147757 Text en © 2016 Nicholson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nicholson, Bryon A. West, Aaron C. Mangiamele, Paul Barbieri, Nicolle Wannemuehler, Yvonne Nolan, Lisa K. Logue, Catherine M. Li, Ganwu Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC |
title | Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC |
title_full | Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC |
title_fullStr | Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC |
title_full_unstemmed | Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC |
title_short | Genetic Characterization of ExPEC-Like Virulence Plasmids among a Subset of NMEC |
title_sort | genetic characterization of expec-like virulence plasmids among a subset of nmec |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723317/ https://www.ncbi.nlm.nih.gov/pubmed/26800268 http://dx.doi.org/10.1371/journal.pone.0147757 |
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