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Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda
BACKGROUND: Mycobacterium tuberculosis (Mtb) is reported to infect about a third of the world’s population but only 10 % are thought to develop active tuberculosis (TB) disease. Host immunity regulated by human leukocyte antigens (HLA) is an important determinant of the outcome of the disease. Here...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4724396/ https://www.ncbi.nlm.nih.gov/pubmed/26803588 http://dx.doi.org/10.1186/s12879-016-1346-0 |
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author | Wamala, Dan Buteme, Helen Koyokoyo Kirimunda, Samuel Kallenius, Gunilla Joloba, Moses |
author_facet | Wamala, Dan Buteme, Helen Koyokoyo Kirimunda, Samuel Kallenius, Gunilla Joloba, Moses |
author_sort | Wamala, Dan |
collection | PubMed |
description | BACKGROUND: Mycobacterium tuberculosis (Mtb) is reported to infect about a third of the world’s population but only 10 % are thought to develop active tuberculosis (TB) disease. Host immunity regulated by human leukocyte antigens (HLA) is an important determinant of the outcome of the disease. Here we investigate HLA class II gene polymorphisms in susceptibility to TB, and whether particular HLA class II alleles were associated with TB in Uganda. METHODS: HIV negative patients with pulmonary TB (n = 43) and genetically related healthy household controls (n = 42) were typed for their HLA II class alleles using polymerase chain reaction sequence specific primer amplification. RESULTS: The HLA-DQB1*03:03 allele was significantly less frequent in patients compared to healthy controls (10 % in controls versus 0 % in patients, p = 0.003). After correction for multiple comparisons the difference remained significant (p = 0.018). CONCLUSIONS: Our results suggest that the HLA-DQB1*03:03 allele may be associated with resistance to TB. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-016-1346-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4724396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47243962016-01-25 Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda Wamala, Dan Buteme, Helen Koyokoyo Kirimunda, Samuel Kallenius, Gunilla Joloba, Moses BMC Infect Dis Research Article BACKGROUND: Mycobacterium tuberculosis (Mtb) is reported to infect about a third of the world’s population but only 10 % are thought to develop active tuberculosis (TB) disease. Host immunity regulated by human leukocyte antigens (HLA) is an important determinant of the outcome of the disease. Here we investigate HLA class II gene polymorphisms in susceptibility to TB, and whether particular HLA class II alleles were associated with TB in Uganda. METHODS: HIV negative patients with pulmonary TB (n = 43) and genetically related healthy household controls (n = 42) were typed for their HLA II class alleles using polymerase chain reaction sequence specific primer amplification. RESULTS: The HLA-DQB1*03:03 allele was significantly less frequent in patients compared to healthy controls (10 % in controls versus 0 % in patients, p = 0.003). After correction for multiple comparisons the difference remained significant (p = 0.018). CONCLUSIONS: Our results suggest that the HLA-DQB1*03:03 allele may be associated with resistance to TB. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-016-1346-0) contains supplementary material, which is available to authorized users. BioMed Central 2016-01-23 /pmc/articles/PMC4724396/ /pubmed/26803588 http://dx.doi.org/10.1186/s12879-016-1346-0 Text en © Wamala et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wamala, Dan Buteme, Helen Koyokoyo Kirimunda, Samuel Kallenius, Gunilla Joloba, Moses Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda |
title | Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda |
title_full | Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda |
title_fullStr | Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda |
title_full_unstemmed | Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda |
title_short | Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda |
title_sort | association between human leukocyte antigen class ii and pulmonary tuberculosis due to mycobacterium tuberculosis in uganda |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4724396/ https://www.ncbi.nlm.nih.gov/pubmed/26803588 http://dx.doi.org/10.1186/s12879-016-1346-0 |
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