Cargando…
An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis
Metabolism consists of biochemical reactions that are combined to generate a robust metabolic network that can respond to perturbations and also adapt to changing environmental conditions. Escherichia coli and Salmonella enterica are closely related enterobacteria that share metabolic components, pa...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725005/ https://www.ncbi.nlm.nih.gov/pubmed/26733068 http://dx.doi.org/10.1128/mBio.01840-15 |
_version_ | 1782411603731283968 |
---|---|
author | Bazurto, Jannell V. Farley, Kristen R. Downs, Diana M. |
author_facet | Bazurto, Jannell V. Farley, Kristen R. Downs, Diana M. |
author_sort | Bazurto, Jannell V. |
collection | PubMed |
description | Metabolism consists of biochemical reactions that are combined to generate a robust metabolic network that can respond to perturbations and also adapt to changing environmental conditions. Escherichia coli and Salmonella enterica are closely related enterobacteria that share metabolic components, pathway structures, and regulatory strategies. The synthesis of thiamine in S. enterica has been used to define a node of the metabolic network by analyzing alternative inputs to thiamine synthesis from diverse metabolic pathways. To assess the conservation of metabolic networks in organisms with highly conserved components, metabolic contributions to thiamine synthesis in E. coli were investigated. Unexpectedly, we found that, unlike S. enterica, E. coli does not use the phosphoribosylpyrophosphate (PRPP) amidotransferase (PurF) as the primary enzyme for synthesis of phosphoribosylamine (PRA). In fact, our data showed that up to 50% of the PRA used by E. coli to make thiamine requires the activities of threonine dehydratase (IlvA) and anthranilate synthase component II (TrpD). Significantly, the IlvA- and TrpD-dependent pathway to PRA functions in S. enterica only in the absence of a functional reactive intermediate deaminase (RidA) enzyme, bringing into focus how these closely related bacteria have distinct metabolic networks. |
format | Online Article Text |
id | pubmed-4725005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-47250052016-01-28 An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis Bazurto, Jannell V. Farley, Kristen R. Downs, Diana M. mBio Research Article Metabolism consists of biochemical reactions that are combined to generate a robust metabolic network that can respond to perturbations and also adapt to changing environmental conditions. Escherichia coli and Salmonella enterica are closely related enterobacteria that share metabolic components, pathway structures, and regulatory strategies. The synthesis of thiamine in S. enterica has been used to define a node of the metabolic network by analyzing alternative inputs to thiamine synthesis from diverse metabolic pathways. To assess the conservation of metabolic networks in organisms with highly conserved components, metabolic contributions to thiamine synthesis in E. coli were investigated. Unexpectedly, we found that, unlike S. enterica, E. coli does not use the phosphoribosylpyrophosphate (PRPP) amidotransferase (PurF) as the primary enzyme for synthesis of phosphoribosylamine (PRA). In fact, our data showed that up to 50% of the PRA used by E. coli to make thiamine requires the activities of threonine dehydratase (IlvA) and anthranilate synthase component II (TrpD). Significantly, the IlvA- and TrpD-dependent pathway to PRA functions in S. enterica only in the absence of a functional reactive intermediate deaminase (RidA) enzyme, bringing into focus how these closely related bacteria have distinct metabolic networks. American Society of Microbiology 2016-01-05 /pmc/articles/PMC4725005/ /pubmed/26733068 http://dx.doi.org/10.1128/mBio.01840-15 Text en Copyright © 2016 Bazurto et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bazurto, Jannell V. Farley, Kristen R. Downs, Diana M. An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis |
title | An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis |
title_full | An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis |
title_fullStr | An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis |
title_full_unstemmed | An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis |
title_short | An Unexpected Route to an Essential Cofactor: Escherichia coli Relies on Threonine for Thiamine Biosynthesis |
title_sort | unexpected route to an essential cofactor: escherichia coli relies on threonine for thiamine biosynthesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725005/ https://www.ncbi.nlm.nih.gov/pubmed/26733068 http://dx.doi.org/10.1128/mBio.01840-15 |
work_keys_str_mv | AT bazurtojannellv anunexpectedroutetoanessentialcofactorescherichiacolireliesonthreonineforthiaminebiosynthesis AT farleykristenr anunexpectedroutetoanessentialcofactorescherichiacolireliesonthreonineforthiaminebiosynthesis AT downsdianam anunexpectedroutetoanessentialcofactorescherichiacolireliesonthreonineforthiaminebiosynthesis AT bazurtojannellv unexpectedroutetoanessentialcofactorescherichiacolireliesonthreonineforthiaminebiosynthesis AT farleykristenr unexpectedroutetoanessentialcofactorescherichiacolireliesonthreonineforthiaminebiosynthesis AT downsdianam unexpectedroutetoanessentialcofactorescherichiacolireliesonthreonineforthiaminebiosynthesis |