Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)

In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68,...

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Autores principales: Zhang, Tiantian, Cui, Xuejiao, Zhang, Jincheng, Wang, Hui, Wu, Meng, Zeng, Hua, Cao, Yuanyuan, Liu, Jingze, Hu, Yonghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2015
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725241/
https://www.ncbi.nlm.nih.gov/pubmed/26797451
http://dx.doi.org/10.3347/kjp.2015.53.6.789
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author Zhang, Tiantian
Cui, Xuejiao
Zhang, Jincheng
Wang, Hui
Wu, Meng
Zeng, Hua
Cao, Yuanyuan
Liu, Jingze
Hu, Yonghong
author_facet Zhang, Tiantian
Cui, Xuejiao
Zhang, Jincheng
Wang, Hui
Wu, Meng
Zeng, Hua
Cao, Yuanyuan
Liu, Jingze
Hu, Yonghong
author_sort Zhang, Tiantian
collection PubMed
description In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
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spelling pubmed-47252412016-01-26 Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae) Zhang, Tiantian Cui, Xuejiao Zhang, Jincheng Wang, Hui Wu, Meng Zeng, Hua Cao, Yuanyuan Liu, Jingze Hu, Yonghong Korean J Parasitol Brief Communication In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum. The Korean Society for Parasitology and Tropical Medicine 2015-12 2015-12-31 /pmc/articles/PMC4725241/ /pubmed/26797451 http://dx.doi.org/10.3347/kjp.2015.53.6.789 Text en © 2015, Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Zhang, Tiantian
Cui, Xuejiao
Zhang, Jincheng
Wang, Hui
Wu, Meng
Zeng, Hua
Cao, Yuanyuan
Liu, Jingze
Hu, Yonghong
Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
title Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
title_full Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
title_fullStr Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
title_full_unstemmed Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
title_short Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
title_sort screening and identification of antigenic proteins from the hard tick dermacentor silvarum (acari: ixodidae)
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725241/
https://www.ncbi.nlm.nih.gov/pubmed/26797451
http://dx.doi.org/10.3347/kjp.2015.53.6.789
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