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Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients

INTRODUCTION: Central nervous system (CNS) infections are life-threatening diseases caused by viral, bacterial, parasitic and fungal microorganisms. The aim of this study was to determine the accuracy of universal polymerase chain reaction (PCR) for the detection of bacterial meningitis among patien...

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Autores principales: Moayedi, Ali Reza, Nejatizadeh, Abdolazim, Mohammadian, Maryam, Rahmati, Mohammad Bagher, Namardizadeh, Vahideh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Electronic physician 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725414/
https://www.ncbi.nlm.nih.gov/pubmed/26816587
http://dx.doi.org/10.19082/1609
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author Moayedi, Ali Reza
Nejatizadeh, Abdolazim
Mohammadian, Maryam
Rahmati, Mohammad Bagher
Namardizadeh, Vahideh
author_facet Moayedi, Ali Reza
Nejatizadeh, Abdolazim
Mohammadian, Maryam
Rahmati, Mohammad Bagher
Namardizadeh, Vahideh
author_sort Moayedi, Ali Reza
collection PubMed
description INTRODUCTION: Central nervous system (CNS) infections are life-threatening diseases caused by viral, bacterial, parasitic and fungal microorganisms. The aim of this study was to determine the accuracy of universal polymerase chain reaction (PCR) for the detection of bacterial meningitis among patients who were referred to Koodakan Hospital in Bandar Abbas because they were suspected of having the disease. METHODS: This study was conducted in 2013 on the patients who were admitted to Bandar Abbas’ Koodakan Hospital because they were suspected of having meningitis. A questionnaire, including demographic data, was completed for each patient. Universal PCR, Cerebrospinal fluid (CSF) analysis, and gram staining and cultures were done for all the patients. The data were analyzed using SPSS software. RESULTS: Among the 100 patients studied 59 (59%) were male and 41 (41%) were female. No patient in our study had a positive smear and culture for meningitis. Among the patients with negative smears and cultures six (6%) had positive universal PCR, and 94 (94%) had negative universal PCR. Based on these results, PCR had 95% specificity and 100% negative predictive value for the prediction of meningitis. In 30 patients (30%), the biochemical analysis of CSF were in favor of meningitis. Among the 30 patients, six patients (20%) had positive universal PCR and 24 patients (80%) had negative universal PCR. CONCLUSION: Based on our results, the universal PCR test is useful in the diagnosis of bacterial meningitis in children. We recommend using it in combination with other tests, such as CSF analysis, for diagnosis of bacterial meningitis.
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spelling pubmed-47254142016-01-26 Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients Moayedi, Ali Reza Nejatizadeh, Abdolazim Mohammadian, Maryam Rahmati, Mohammad Bagher Namardizadeh, Vahideh Electron Physician Original Article INTRODUCTION: Central nervous system (CNS) infections are life-threatening diseases caused by viral, bacterial, parasitic and fungal microorganisms. The aim of this study was to determine the accuracy of universal polymerase chain reaction (PCR) for the detection of bacterial meningitis among patients who were referred to Koodakan Hospital in Bandar Abbas because they were suspected of having the disease. METHODS: This study was conducted in 2013 on the patients who were admitted to Bandar Abbas’ Koodakan Hospital because they were suspected of having meningitis. A questionnaire, including demographic data, was completed for each patient. Universal PCR, Cerebrospinal fluid (CSF) analysis, and gram staining and cultures were done for all the patients. The data were analyzed using SPSS software. RESULTS: Among the 100 patients studied 59 (59%) were male and 41 (41%) were female. No patient in our study had a positive smear and culture for meningitis. Among the patients with negative smears and cultures six (6%) had positive universal PCR, and 94 (94%) had negative universal PCR. Based on these results, PCR had 95% specificity and 100% negative predictive value for the prediction of meningitis. In 30 patients (30%), the biochemical analysis of CSF were in favor of meningitis. Among the 30 patients, six patients (20%) had positive universal PCR and 24 patients (80%) had negative universal PCR. CONCLUSION: Based on our results, the universal PCR test is useful in the diagnosis of bacterial meningitis in children. We recommend using it in combination with other tests, such as CSF analysis, for diagnosis of bacterial meningitis. Electronic physician 2015-12-20 /pmc/articles/PMC4725414/ /pubmed/26816587 http://dx.doi.org/10.19082/1609 Text en © 2015 The Authors This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/3.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Original Article
Moayedi, Ali Reza
Nejatizadeh, Abdolazim
Mohammadian, Maryam
Rahmati, Mohammad Bagher
Namardizadeh, Vahideh
Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients
title Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients
title_full Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients
title_fullStr Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients
title_full_unstemmed Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients
title_short Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients
title_sort accuracy of universal polymerase chain reaction (pcr) for detection of bacterial meningitis among suspected patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725414/
https://www.ncbi.nlm.nih.gov/pubmed/26816587
http://dx.doi.org/10.19082/1609
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