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IL10 in Lupus Nephritis: Detection and relationship with disease activity

INTRODUCTION: Glomerulonephritis is a major determinant of the course and prognosis of systemic lupus erythematosus (SLE) and is evident in 40%–85% of patients. IL10, a cytokine produced by monocytes and-to a lesser extent-lymphocytes, has pleiotropic effects in immune regulation and inflammation. I...

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Autores principales: Zeid, Sameh Abou, Khalifa, Ghada, Nabil, Malak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Electronic physician 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725424/
https://www.ncbi.nlm.nih.gov/pubmed/26816594
http://dx.doi.org/10.19082/1680
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author Zeid, Sameh Abou
Khalifa, Ghada
Nabil, Malak
author_facet Zeid, Sameh Abou
Khalifa, Ghada
Nabil, Malak
author_sort Zeid, Sameh Abou
collection PubMed
description INTRODUCTION: Glomerulonephritis is a major determinant of the course and prognosis of systemic lupus erythematosus (SLE) and is evident in 40%–85% of patients. IL10, a cytokine produced by monocytes and-to a lesser extent-lymphocytes, has pleiotropic effects in immune regulation and inflammation. It enhances B cell survival, proliferation, differentiation, and antibody production; these effects play a role in autoimmune diseases. Among identified polymorphisms in the IL10 promoter, three linked single nucleotide polymorphisms (SNPs) of −1082 G/A, 819 T/C, and −592 A/C have been shown to influence the IL10 gene expression. Compared with the −592 C allele, the 592 A is associated with lower IL10 production in vitro. The objectives of this study were to investigate the −592 A/C polymorphism in patients with and without lupus nephritis and to assess its influence on IL10 secretion in vivo and its role in pathogenesis and clinicopathological characteristics of lupus nephritis. METHODS: This case control study was conducted on 40 SLE patients recruited for the study from those attending the nephrology department of the Theodor Bilharz Research Institute (outpatient clinic and inpatient ward) in 2013. Patients were divided into two groups, group I (SLE patients without evidence of nephritis) and group II (SLE patients with lupus nephritis). Data were analyzed using SPSS (version 12), a t-test, Chi square, ANOVA, and the Pearson product–moment correlation coefficient. RESULTS: Our study found an increase in IL10 serum in lupus nephritis patients compared to those without renal involvement (without statistical significance). No significant differences emerged in the level of IL10 serum among different pathological classes. CONCLUSION: The IL10 gene (−592 A/C) polymorphism, though not associated with lupus nephritis’s susceptibility in the present study, does play a role.
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spelling pubmed-47254242016-01-26 IL10 in Lupus Nephritis: Detection and relationship with disease activity Zeid, Sameh Abou Khalifa, Ghada Nabil, Malak Electron Physician Original Article INTRODUCTION: Glomerulonephritis is a major determinant of the course and prognosis of systemic lupus erythematosus (SLE) and is evident in 40%–85% of patients. IL10, a cytokine produced by monocytes and-to a lesser extent-lymphocytes, has pleiotropic effects in immune regulation and inflammation. It enhances B cell survival, proliferation, differentiation, and antibody production; these effects play a role in autoimmune diseases. Among identified polymorphisms in the IL10 promoter, three linked single nucleotide polymorphisms (SNPs) of −1082 G/A, 819 T/C, and −592 A/C have been shown to influence the IL10 gene expression. Compared with the −592 C allele, the 592 A is associated with lower IL10 production in vitro. The objectives of this study were to investigate the −592 A/C polymorphism in patients with and without lupus nephritis and to assess its influence on IL10 secretion in vivo and its role in pathogenesis and clinicopathological characteristics of lupus nephritis. METHODS: This case control study was conducted on 40 SLE patients recruited for the study from those attending the nephrology department of the Theodor Bilharz Research Institute (outpatient clinic and inpatient ward) in 2013. Patients were divided into two groups, group I (SLE patients without evidence of nephritis) and group II (SLE patients with lupus nephritis). Data were analyzed using SPSS (version 12), a t-test, Chi square, ANOVA, and the Pearson product–moment correlation coefficient. RESULTS: Our study found an increase in IL10 serum in lupus nephritis patients compared to those without renal involvement (without statistical significance). No significant differences emerged in the level of IL10 serum among different pathological classes. CONCLUSION: The IL10 gene (−592 A/C) polymorphism, though not associated with lupus nephritis’s susceptibility in the present study, does play a role. Electronic physician 2015-12-20 /pmc/articles/PMC4725424/ /pubmed/26816594 http://dx.doi.org/10.19082/1680 Text en © 2015 The Authors This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/3.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Original Article
Zeid, Sameh Abou
Khalifa, Ghada
Nabil, Malak
IL10 in Lupus Nephritis: Detection and relationship with disease activity
title IL10 in Lupus Nephritis: Detection and relationship with disease activity
title_full IL10 in Lupus Nephritis: Detection and relationship with disease activity
title_fullStr IL10 in Lupus Nephritis: Detection and relationship with disease activity
title_full_unstemmed IL10 in Lupus Nephritis: Detection and relationship with disease activity
title_short IL10 in Lupus Nephritis: Detection and relationship with disease activity
title_sort il10 in lupus nephritis: detection and relationship with disease activity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725424/
https://www.ncbi.nlm.nih.gov/pubmed/26816594
http://dx.doi.org/10.19082/1680
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