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Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness

Recent studies have shown that rates of bacterial dispersion in soils are controlled by hydration conditions that define size and connectivity of the retained aqueous phase. Despite the ecological implications of such constraints, microscale observations of this phenomenon remain scarce. Here, we qu...

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Autores principales: Tecon, Robin, Or, Dani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725831/
https://www.ncbi.nlm.nih.gov/pubmed/26757676
http://dx.doi.org/10.1038/srep19409
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author Tecon, Robin
Or, Dani
author_facet Tecon, Robin
Or, Dani
author_sort Tecon, Robin
collection PubMed
description Recent studies have shown that rates of bacterial dispersion in soils are controlled by hydration conditions that define size and connectivity of the retained aqueous phase. Despite the ecological implications of such constraints, microscale observations of this phenomenon remain scarce. Here, we quantified aqueous film characteristics and bacterial flagellated motility in response to systematic variations in microhydrological conditions on porous ceramic surfaces that mimic unsaturated soils. We directly measured aqueous film thickness and documented its microscale heterogeneity. Flagellar motility was controlled by surface hydration conditions, as cell velocity decreased and dispersion practically ceased at water potentials exceeding –2 kPa (resulting in thinner and disconnected liquid films). The fragmentation of aquatic habitats was delineated indirectly through bacterial dispersal distances within connected aqueous clusters. We documented bacterial dispersal radii ranging from 100 to 10 μm as the water potential varied from 0 to –7 kPa, respectively. The observed decrease of flagellated velocity and dispersal ranges at lower matric potentials were in good agreement with mechanistic model predictions. Hydration-restricted habitats thus play significant role in bacterial motility and dispersal, which has potentially important impact on soil microbial ecology and diversity.
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spelling pubmed-47258312016-01-28 Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness Tecon, Robin Or, Dani Sci Rep Article Recent studies have shown that rates of bacterial dispersion in soils are controlled by hydration conditions that define size and connectivity of the retained aqueous phase. Despite the ecological implications of such constraints, microscale observations of this phenomenon remain scarce. Here, we quantified aqueous film characteristics and bacterial flagellated motility in response to systematic variations in microhydrological conditions on porous ceramic surfaces that mimic unsaturated soils. We directly measured aqueous film thickness and documented its microscale heterogeneity. Flagellar motility was controlled by surface hydration conditions, as cell velocity decreased and dispersion practically ceased at water potentials exceeding –2 kPa (resulting in thinner and disconnected liquid films). The fragmentation of aquatic habitats was delineated indirectly through bacterial dispersal distances within connected aqueous clusters. We documented bacterial dispersal radii ranging from 100 to 10 μm as the water potential varied from 0 to –7 kPa, respectively. The observed decrease of flagellated velocity and dispersal ranges at lower matric potentials were in good agreement with mechanistic model predictions. Hydration-restricted habitats thus play significant role in bacterial motility and dispersal, which has potentially important impact on soil microbial ecology and diversity. Nature Publishing Group 2016-01-13 /pmc/articles/PMC4725831/ /pubmed/26757676 http://dx.doi.org/10.1038/srep19409 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tecon, Robin
Or, Dani
Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
title Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
title_full Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
title_fullStr Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
title_full_unstemmed Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
title_short Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
title_sort bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725831/
https://www.ncbi.nlm.nih.gov/pubmed/26757676
http://dx.doi.org/10.1038/srep19409
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