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Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing

Tolerance of alfalfa (Medicago sativa L.) to animal grazing varies widely within the species. However, the molecular mechanisms influencing the grazing tolerant phenotype remain uncharacterized. The objective of this study was to identify genes and pathways that control grazing response in alfalfa....

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Autores principales: Wang, Junjie, Zhao, Yan, Ray, Ian, Song, Mingzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725929/
https://www.ncbi.nlm.nih.gov/pubmed/26763747
http://dx.doi.org/10.1038/srep19438
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author Wang, Junjie
Zhao, Yan
Ray, Ian
Song, Mingzhou
author_facet Wang, Junjie
Zhao, Yan
Ray, Ian
Song, Mingzhou
author_sort Wang, Junjie
collection PubMed
description Tolerance of alfalfa (Medicago sativa L.) to animal grazing varies widely within the species. However, the molecular mechanisms influencing the grazing tolerant phenotype remain uncharacterized. The objective of this study was to identify genes and pathways that control grazing response in alfalfa. We analyzed whole-plant de novo transcriptomes from grazing tolerant and intolerant populations of M. sativa ssp. falcata subjected to grazing by sheep. Among the Gene Ontology terms which were identified as grazing responsive in the tolerant plants and differentially enriched between the tolerant and intolerant populations (both grazed), most were associated with the ribosome and translation-related activities, cell wall processes, and response to oxygen levels. Twenty-one grazing responsive pathways were identified that also exhibited differential expression between the tolerant and intolerant populations. These pathways were associated with secondary metabolite production, primary carbohydrate metabolic pathways, shikimate derivative dependent pathways, ribosomal subunit composition, hormone signaling, wound response, cell wall formation, and anti-oxidant defense. Sequence polymorphisms were detected among several differentially expressed homologous transcripts between the tolerant and intolerant populations. These differentially responsive genes and pathways constitute potential response mechanisms for grazing tolerance in alfalfa. They also provide potential targets for molecular breeding efforts to develop grazing-tolerant cultivars of alfalfa.
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spelling pubmed-47259292016-01-28 Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing Wang, Junjie Zhao, Yan Ray, Ian Song, Mingzhou Sci Rep Article Tolerance of alfalfa (Medicago sativa L.) to animal grazing varies widely within the species. However, the molecular mechanisms influencing the grazing tolerant phenotype remain uncharacterized. The objective of this study was to identify genes and pathways that control grazing response in alfalfa. We analyzed whole-plant de novo transcriptomes from grazing tolerant and intolerant populations of M. sativa ssp. falcata subjected to grazing by sheep. Among the Gene Ontology terms which were identified as grazing responsive in the tolerant plants and differentially enriched between the tolerant and intolerant populations (both grazed), most were associated with the ribosome and translation-related activities, cell wall processes, and response to oxygen levels. Twenty-one grazing responsive pathways were identified that also exhibited differential expression between the tolerant and intolerant populations. These pathways were associated with secondary metabolite production, primary carbohydrate metabolic pathways, shikimate derivative dependent pathways, ribosomal subunit composition, hormone signaling, wound response, cell wall formation, and anti-oxidant defense. Sequence polymorphisms were detected among several differentially expressed homologous transcripts between the tolerant and intolerant populations. These differentially responsive genes and pathways constitute potential response mechanisms for grazing tolerance in alfalfa. They also provide potential targets for molecular breeding efforts to develop grazing-tolerant cultivars of alfalfa. Nature Publishing Group 2016-01-14 /pmc/articles/PMC4725929/ /pubmed/26763747 http://dx.doi.org/10.1038/srep19438 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Junjie
Zhao, Yan
Ray, Ian
Song, Mingzhou
Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
title Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
title_full Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
title_fullStr Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
title_full_unstemmed Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
title_short Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
title_sort transcriptome responses in alfalfa associated with tolerance to intensive animal grazing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725929/
https://www.ncbi.nlm.nih.gov/pubmed/26763747
http://dx.doi.org/10.1038/srep19438
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