Cargando…

Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia

Preeclampsia is a disease of pregnancy involving systemic endothelial dysfunction. However, cardiovascular consequences of preeclampsia are difficult to analyze in humans. The objective of the present study is to evaluate the cardiovascular dysfunction induced by preeclampsia by examining the endoth...

Descripción completa

Detalles Bibliográficos
Autores principales: Ducat, Aurélien, Doridot, Ludivine, Calicchio, Rosamaria, Méhats, Celine, Vilotte, Jean-Luc, Castille, Johann, Barbaux, Sandrine, Couderc, Betty, Jacques, Sébastien, Letourneur, Franck, Buffat, Christophe, Le Grand, Fabien, Laissue, Paul, Miralles, Francisco, Vaiman, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725931/
https://www.ncbi.nlm.nih.gov/pubmed/26758611
http://dx.doi.org/10.1038/srep19196
_version_ 1782411709671014400
author Ducat, Aurélien
Doridot, Ludivine
Calicchio, Rosamaria
Méhats, Celine
Vilotte, Jean-Luc
Castille, Johann
Barbaux, Sandrine
Couderc, Betty
Jacques, Sébastien
Letourneur, Franck
Buffat, Christophe
Le Grand, Fabien
Laissue, Paul
Miralles, Francisco
Vaiman, Daniel
author_facet Ducat, Aurélien
Doridot, Ludivine
Calicchio, Rosamaria
Méhats, Celine
Vilotte, Jean-Luc
Castille, Johann
Barbaux, Sandrine
Couderc, Betty
Jacques, Sébastien
Letourneur, Franck
Buffat, Christophe
Le Grand, Fabien
Laissue, Paul
Miralles, Francisco
Vaiman, Daniel
author_sort Ducat, Aurélien
collection PubMed
description Preeclampsia is a disease of pregnancy involving systemic endothelial dysfunction. However, cardiovascular consequences of preeclampsia are difficult to analyze in humans. The objective of the present study is to evaluate the cardiovascular dysfunction induced by preeclampsia by examining the endothelium of mice suffering of severe preeclampsia induced by STOX1 overexpression. Using Next Generation Sequencing on endothelial cells of mice carrying either transgenic or control embryos, we discovered significant alterations of gene networks involved in inflammation, cell cycle, and cardiac hypertrophy. In addition, the heart of the preeclamptic mice revealed cardiac hypertrophy associated with histological anomalies. Bioinformatics comparison of the networks of modified genes in the endothelial cells of the preeclamptic mice and HUVECs exposed to plasma from preeclamptic women identified striking similarities. The cardiovascular alterations in the pregnant mice are comparable to those endured by the cardiovascular system of preeclamptic women. The STOX1 mice could help to better understand the endothelial dysfunction in the context of preeclampsia, and guide the search for efficient therapies able to protect the maternal endothelium during the disease and its aftermath.
format Online
Article
Text
id pubmed-4725931
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-47259312016-01-28 Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia Ducat, Aurélien Doridot, Ludivine Calicchio, Rosamaria Méhats, Celine Vilotte, Jean-Luc Castille, Johann Barbaux, Sandrine Couderc, Betty Jacques, Sébastien Letourneur, Franck Buffat, Christophe Le Grand, Fabien Laissue, Paul Miralles, Francisco Vaiman, Daniel Sci Rep Article Preeclampsia is a disease of pregnancy involving systemic endothelial dysfunction. However, cardiovascular consequences of preeclampsia are difficult to analyze in humans. The objective of the present study is to evaluate the cardiovascular dysfunction induced by preeclampsia by examining the endothelium of mice suffering of severe preeclampsia induced by STOX1 overexpression. Using Next Generation Sequencing on endothelial cells of mice carrying either transgenic or control embryos, we discovered significant alterations of gene networks involved in inflammation, cell cycle, and cardiac hypertrophy. In addition, the heart of the preeclamptic mice revealed cardiac hypertrophy associated with histological anomalies. Bioinformatics comparison of the networks of modified genes in the endothelial cells of the preeclamptic mice and HUVECs exposed to plasma from preeclamptic women identified striking similarities. The cardiovascular alterations in the pregnant mice are comparable to those endured by the cardiovascular system of preeclamptic women. The STOX1 mice could help to better understand the endothelial dysfunction in the context of preeclampsia, and guide the search for efficient therapies able to protect the maternal endothelium during the disease and its aftermath. Nature Publishing Group 2016-01-13 /pmc/articles/PMC4725931/ /pubmed/26758611 http://dx.doi.org/10.1038/srep19196 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ducat, Aurélien
Doridot, Ludivine
Calicchio, Rosamaria
Méhats, Celine
Vilotte, Jean-Luc
Castille, Johann
Barbaux, Sandrine
Couderc, Betty
Jacques, Sébastien
Letourneur, Franck
Buffat, Christophe
Le Grand, Fabien
Laissue, Paul
Miralles, Francisco
Vaiman, Daniel
Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia
title Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia
title_full Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia
title_fullStr Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia
title_full_unstemmed Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia
title_short Endothelial cell dysfunction and cardiac hypertrophy in the STOX1 model of preeclampsia
title_sort endothelial cell dysfunction and cardiac hypertrophy in the stox1 model of preeclampsia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725931/
https://www.ncbi.nlm.nih.gov/pubmed/26758611
http://dx.doi.org/10.1038/srep19196
work_keys_str_mv AT ducataurelien endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT doridotludivine endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT calicchiorosamaria endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT mehatsceline endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT vilottejeanluc endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT castillejohann endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT barbauxsandrine endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT coudercbetty endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT jacquessebastien endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT letourneurfranck endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT buffatchristophe endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT legrandfabien endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT laissuepaul endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT mirallesfrancisco endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia
AT vaimandaniel endothelialcelldysfunctionandcardiachypertrophyinthestox1modelofpreeclampsia