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Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells

Mumps virus (MuV) infection frequently causes orchitis and impairs male fertility. However, the mechanisms underlying the innate immune responses to MuV infection in the testis have yet to be investigated. This study showed that MuV induced innate immune responses in mouse Sertoli and Leydig cells t...

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Autores principales: Wu, Han, Shi, Lili, Wang, Qing, Cheng, Lijing, Zhao, Xiang, Chen, Qiaoyuan, Jiang, Qian, Feng, Min, Li, Qihan, Han, Daishu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725973/
https://www.ncbi.nlm.nih.gov/pubmed/26776505
http://dx.doi.org/10.1038/srep19507
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author Wu, Han
Shi, Lili
Wang, Qing
Cheng, Lijing
Zhao, Xiang
Chen, Qiaoyuan
Jiang, Qian
Feng, Min
Li, Qihan
Han, Daishu
author_facet Wu, Han
Shi, Lili
Wang, Qing
Cheng, Lijing
Zhao, Xiang
Chen, Qiaoyuan
Jiang, Qian
Feng, Min
Li, Qihan
Han, Daishu
author_sort Wu, Han
collection PubMed
description Mumps virus (MuV) infection frequently causes orchitis and impairs male fertility. However, the mechanisms underlying the innate immune responses to MuV infection in the testis have yet to be investigated. This study showed that MuV induced innate immune responses in mouse Sertoli and Leydig cells through TLR2 and retinoic acid-inducible gene I (RIG-I) signaling, which result in the production of proinflammatory cytokines and chemokines, including TNF-α, IL-6, MCP-1, CXCL10, and type 1 interferons (IFN-α and IFN-β). By contrast, MuV did not induce the cytokine production in male germ cells. In response to MuV infection, Sertoli cells produced higher levels of proinflammatory cytokines and chemokines but lower levels of type 1 IFNs than Leydig cells did. The MuV-induced cytokine production by Sertoli and Leydig cells was significantly reduced by the knockout of TLR2 or the knockdown of RIG-I signaling. The local injection of MuV into the testis triggered the testicular innate immune responses in vivo. Moreover, MuV infection suppressed testosterone synthesis by Leydig cells. This is the first study examining the innate immune responses to MuV infection in testicular cells. The results provide novel insights into the mechanisms underlying the MuV-induced innate immune responses in the testis.
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spelling pubmed-47259732016-01-28 Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells Wu, Han Shi, Lili Wang, Qing Cheng, Lijing Zhao, Xiang Chen, Qiaoyuan Jiang, Qian Feng, Min Li, Qihan Han, Daishu Sci Rep Article Mumps virus (MuV) infection frequently causes orchitis and impairs male fertility. However, the mechanisms underlying the innate immune responses to MuV infection in the testis have yet to be investigated. This study showed that MuV induced innate immune responses in mouse Sertoli and Leydig cells through TLR2 and retinoic acid-inducible gene I (RIG-I) signaling, which result in the production of proinflammatory cytokines and chemokines, including TNF-α, IL-6, MCP-1, CXCL10, and type 1 interferons (IFN-α and IFN-β). By contrast, MuV did not induce the cytokine production in male germ cells. In response to MuV infection, Sertoli cells produced higher levels of proinflammatory cytokines and chemokines but lower levels of type 1 IFNs than Leydig cells did. The MuV-induced cytokine production by Sertoli and Leydig cells was significantly reduced by the knockout of TLR2 or the knockdown of RIG-I signaling. The local injection of MuV into the testis triggered the testicular innate immune responses in vivo. Moreover, MuV infection suppressed testosterone synthesis by Leydig cells. This is the first study examining the innate immune responses to MuV infection in testicular cells. The results provide novel insights into the mechanisms underlying the MuV-induced innate immune responses in the testis. Nature Publishing Group 2016-01-18 /pmc/articles/PMC4725973/ /pubmed/26776505 http://dx.doi.org/10.1038/srep19507 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wu, Han
Shi, Lili
Wang, Qing
Cheng, Lijing
Zhao, Xiang
Chen, Qiaoyuan
Jiang, Qian
Feng, Min
Li, Qihan
Han, Daishu
Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells
title Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells
title_full Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells
title_fullStr Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells
title_full_unstemmed Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells
title_short Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells
title_sort mumps virus-induced innate immune responses in mouse sertoli and leydig cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725973/
https://www.ncbi.nlm.nih.gov/pubmed/26776505
http://dx.doi.org/10.1038/srep19507
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