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Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging
Cells are complex chemical systems, where the molecular composition at different cellular locations and specific intracellular chemical interactions determine the biological function. An in-situ nondestructive characterization of the complicated chemical processes (like e.g. apoptosis) is the goal o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726017/ https://www.ncbi.nlm.nih.gov/pubmed/26781186 http://dx.doi.org/10.1038/srep19173 |
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author | Chen, Yong Bai, Xiangru Su, Le Du, Zhanwei Shen, Aiguo Materny, Arnulf Hu, Jiming |
author_facet | Chen, Yong Bai, Xiangru Su, Le Du, Zhanwei Shen, Aiguo Materny, Arnulf Hu, Jiming |
author_sort | Chen, Yong |
collection | PubMed |
description | Cells are complex chemical systems, where the molecular composition at different cellular locations and specific intracellular chemical interactions determine the biological function. An in-situ nondestructive characterization of the complicated chemical processes (like e.g. apoptosis) is the goal of our study. Here, we present the results of simultaneous and three-dimensional imaging of double organelles (nucleus and membrane) in single HeLa cells by means of either labelled or label-free surface-enhanced Raman spectroscopy (SERS). This combination of imaging with and without labels is not possible when using fluorescence microscopy. The SERS technique is used for a stereoscopic description of the intrinsic chemical nature of nuclei and the precise localization of folate (FA) and luteinizing hormone-releasing hormone (LHRH) on the membrane under highly confocal conditions. We also report on the time-dependent changes of cell nuclei as well as membrane receptor proteins during apoptosis analyzed by statistical multivariate methods. The multiplex three-dimensional SERS imaging technique allows for both temporal (real time) and spatial (multiple organelles and molecules in three-dimensional space) live-cell imaging and therefore provides a new and attractive 2D/3D tracing method in biomedicine on subcellular level. |
format | Online Article Text |
id | pubmed-4726017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47260172016-01-28 Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging Chen, Yong Bai, Xiangru Su, Le Du, Zhanwei Shen, Aiguo Materny, Arnulf Hu, Jiming Sci Rep Article Cells are complex chemical systems, where the molecular composition at different cellular locations and specific intracellular chemical interactions determine the biological function. An in-situ nondestructive characterization of the complicated chemical processes (like e.g. apoptosis) is the goal of our study. Here, we present the results of simultaneous and three-dimensional imaging of double organelles (nucleus and membrane) in single HeLa cells by means of either labelled or label-free surface-enhanced Raman spectroscopy (SERS). This combination of imaging with and without labels is not possible when using fluorescence microscopy. The SERS technique is used for a stereoscopic description of the intrinsic chemical nature of nuclei and the precise localization of folate (FA) and luteinizing hormone-releasing hormone (LHRH) on the membrane under highly confocal conditions. We also report on the time-dependent changes of cell nuclei as well as membrane receptor proteins during apoptosis analyzed by statistical multivariate methods. The multiplex three-dimensional SERS imaging technique allows for both temporal (real time) and spatial (multiple organelles and molecules in three-dimensional space) live-cell imaging and therefore provides a new and attractive 2D/3D tracing method in biomedicine on subcellular level. Nature Publishing Group 2016-01-19 /pmc/articles/PMC4726017/ /pubmed/26781186 http://dx.doi.org/10.1038/srep19173 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chen, Yong Bai, Xiangru Su, Le Du, Zhanwei Shen, Aiguo Materny, Arnulf Hu, Jiming Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging |
title | Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging |
title_full | Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging |
title_fullStr | Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging |
title_full_unstemmed | Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging |
title_short | Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging |
title_sort | combined labelled and label-free sers probes for triplex three-dimensional cellular imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726017/ https://www.ncbi.nlm.nih.gov/pubmed/26781186 http://dx.doi.org/10.1038/srep19173 |
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