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Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?

Implementation of therapy for acute kidney injury (AKI) depends on successful prediction of individual patient prognosis. Clinical markers as serum creatinine (sCr) have limitations in sensitivity and early response. The aim of the study was to identify novel molecules in urine which show altered le...

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Autores principales: Gonzalez-Calero, Laura, Martin-Lorenzo, Marta, Ramos-Barron, Angeles, Ruiz-Criado, Jorge, Maroto, Aroa S., Ortiz, Alberto, Gomez-Alamillo, Carlos, Arias, Manuel, Vivanco, Fernando, Alvarez-Llamas, Gloria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726181/
https://www.ncbi.nlm.nih.gov/pubmed/26792617
http://dx.doi.org/10.1038/srep19667
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author Gonzalez-Calero, Laura
Martin-Lorenzo, Marta
Ramos-Barron, Angeles
Ruiz-Criado, Jorge
Maroto, Aroa S.
Ortiz, Alberto
Gomez-Alamillo, Carlos
Arias, Manuel
Vivanco, Fernando
Alvarez-Llamas, Gloria
author_facet Gonzalez-Calero, Laura
Martin-Lorenzo, Marta
Ramos-Barron, Angeles
Ruiz-Criado, Jorge
Maroto, Aroa S.
Ortiz, Alberto
Gomez-Alamillo, Carlos
Arias, Manuel
Vivanco, Fernando
Alvarez-Llamas, Gloria
author_sort Gonzalez-Calero, Laura
collection PubMed
description Implementation of therapy for acute kidney injury (AKI) depends on successful prediction of individual patient prognosis. Clinical markers as serum creatinine (sCr) have limitations in sensitivity and early response. The aim of the study was to identify novel molecules in urine which show altered levels in response to AKI and investigate their value as predictors of recovery. Changes in the urinary proteome were here investigated in a cohort of 88 subjects (55 AKI patients and 33 healthy donors) grouped in discovery and validation independent cohorts. Patients’ urine was collected at three time points: within the first 48 h after diagnosis(T1), at 7 days of follow-up(T2) and at discharge of Nephrology(T3). Differential gel electrophoresis was performed and data were confirmed by Western blot (WB), liquid chromatography/mass spectrometry (LC-MS/MS) and enzyme-linked immunosorbent assay (ELISA). Retinol binding protein 4 (RBP4) and kininogen-1 (KNG1) were found significantly altered following AKI. RBP4 increased at T1, and progressively decreased towards normalization. Maintained decrease was observed for KNG1 from T1. Individual patient response along time revealed RBP4 responds to recovery earlier than sCr. In conclusion, KNG1 and RBP4 respond to AKI. By monitoring RBP4, patient’s recovery can be anticipated pointing to a role of RBP4 in prognosis evaluation.
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spelling pubmed-47261812016-01-27 Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis? Gonzalez-Calero, Laura Martin-Lorenzo, Marta Ramos-Barron, Angeles Ruiz-Criado, Jorge Maroto, Aroa S. Ortiz, Alberto Gomez-Alamillo, Carlos Arias, Manuel Vivanco, Fernando Alvarez-Llamas, Gloria Sci Rep Article Implementation of therapy for acute kidney injury (AKI) depends on successful prediction of individual patient prognosis. Clinical markers as serum creatinine (sCr) have limitations in sensitivity and early response. The aim of the study was to identify novel molecules in urine which show altered levels in response to AKI and investigate their value as predictors of recovery. Changes in the urinary proteome were here investigated in a cohort of 88 subjects (55 AKI patients and 33 healthy donors) grouped in discovery and validation independent cohorts. Patients’ urine was collected at three time points: within the first 48 h after diagnosis(T1), at 7 days of follow-up(T2) and at discharge of Nephrology(T3). Differential gel electrophoresis was performed and data were confirmed by Western blot (WB), liquid chromatography/mass spectrometry (LC-MS/MS) and enzyme-linked immunosorbent assay (ELISA). Retinol binding protein 4 (RBP4) and kininogen-1 (KNG1) were found significantly altered following AKI. RBP4 increased at T1, and progressively decreased towards normalization. Maintained decrease was observed for KNG1 from T1. Individual patient response along time revealed RBP4 responds to recovery earlier than sCr. In conclusion, KNG1 and RBP4 respond to AKI. By monitoring RBP4, patient’s recovery can be anticipated pointing to a role of RBP4 in prognosis evaluation. Nature Publishing Group 2016-01-21 /pmc/articles/PMC4726181/ /pubmed/26792617 http://dx.doi.org/10.1038/srep19667 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gonzalez-Calero, Laura
Martin-Lorenzo, Marta
Ramos-Barron, Angeles
Ruiz-Criado, Jorge
Maroto, Aroa S.
Ortiz, Alberto
Gomez-Alamillo, Carlos
Arias, Manuel
Vivanco, Fernando
Alvarez-Llamas, Gloria
Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?
title Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?
title_full Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?
title_fullStr Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?
title_full_unstemmed Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?
title_short Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?
title_sort urinary kininogen-1 and retinol binding protein-4 respond to acute kidney injury: predictors of patient prognosis?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726181/
https://www.ncbi.nlm.nih.gov/pubmed/26792617
http://dx.doi.org/10.1038/srep19667
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