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Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity

Non-invasive imaging of lactate is of enormous significance in cancer and metabolic disorders where glycolysis dominates. Here, for the first time, we describe a chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) method (LATEST), based on the exchange between lactate hydro...

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Autores principales: DeBrosse, Catherine, Nanga, Ravi Prakash Reddy, Bagga, Puneet, Nath, Kavindra, Haris, Mohammad, Marincola, Francesco, Schnall, Mitchell D., Hariharan, Hari, Reddy, Ravinder
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726389/
https://www.ncbi.nlm.nih.gov/pubmed/26794265
http://dx.doi.org/10.1038/srep19517
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author DeBrosse, Catherine
Nanga, Ravi Prakash Reddy
Bagga, Puneet
Nath, Kavindra
Haris, Mohammad
Marincola, Francesco
Schnall, Mitchell D.
Hariharan, Hari
Reddy, Ravinder
author_facet DeBrosse, Catherine
Nanga, Ravi Prakash Reddy
Bagga, Puneet
Nath, Kavindra
Haris, Mohammad
Marincola, Francesco
Schnall, Mitchell D.
Hariharan, Hari
Reddy, Ravinder
author_sort DeBrosse, Catherine
collection PubMed
description Non-invasive imaging of lactate is of enormous significance in cancer and metabolic disorders where glycolysis dominates. Here, for the first time, we describe a chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) method (LATEST), based on the exchange between lactate hydroxyl proton and bulk water protons to image lactate with high spatial resolution. We demonstrate the feasibility of imaging lactate with LATEST in lactate phantoms under physiological conditions, in a mouse model of lymphoma tumors, and in skeletal muscle of healthy human subjects pre- and post-exercise. The method is validated by measuring LATEST changes in lymphoma tumors pre- and post-infusion of pyruvate and correlating them with lactate determined from multiple quantum filtered proton magnetic resonance spectroscopy (SEL-MQC (1)H-MRS). Similarly, dynamic LATEST changes in exercising human skeletal muscle are correlated with lactate determined from SEL-MQC (1)H-MRS. The LATEST method does not involve injection of radioactive isotopes or labeled metabolites. It has over two orders of magnitude higher sensitivity compared to conventional (1)H-MRS. It is anticipated that this technique will have a wide range of applications including diagnosis and evaluation of therapeutic response of cancer, diabetes, cardiac, and musculoskeletal diseases. The advantages of LATEST over existing methods and its potential challenges are discussed.
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spelling pubmed-47263892016-01-27 Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity DeBrosse, Catherine Nanga, Ravi Prakash Reddy Bagga, Puneet Nath, Kavindra Haris, Mohammad Marincola, Francesco Schnall, Mitchell D. Hariharan, Hari Reddy, Ravinder Sci Rep Article Non-invasive imaging of lactate is of enormous significance in cancer and metabolic disorders where glycolysis dominates. Here, for the first time, we describe a chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) method (LATEST), based on the exchange between lactate hydroxyl proton and bulk water protons to image lactate with high spatial resolution. We demonstrate the feasibility of imaging lactate with LATEST in lactate phantoms under physiological conditions, in a mouse model of lymphoma tumors, and in skeletal muscle of healthy human subjects pre- and post-exercise. The method is validated by measuring LATEST changes in lymphoma tumors pre- and post-infusion of pyruvate and correlating them with lactate determined from multiple quantum filtered proton magnetic resonance spectroscopy (SEL-MQC (1)H-MRS). Similarly, dynamic LATEST changes in exercising human skeletal muscle are correlated with lactate determined from SEL-MQC (1)H-MRS. The LATEST method does not involve injection of radioactive isotopes or labeled metabolites. It has over two orders of magnitude higher sensitivity compared to conventional (1)H-MRS. It is anticipated that this technique will have a wide range of applications including diagnosis and evaluation of therapeutic response of cancer, diabetes, cardiac, and musculoskeletal diseases. The advantages of LATEST over existing methods and its potential challenges are discussed. Nature Publishing Group 2016-01-22 /pmc/articles/PMC4726389/ /pubmed/26794265 http://dx.doi.org/10.1038/srep19517 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
DeBrosse, Catherine
Nanga, Ravi Prakash Reddy
Bagga, Puneet
Nath, Kavindra
Haris, Mohammad
Marincola, Francesco
Schnall, Mitchell D.
Hariharan, Hari
Reddy, Ravinder
Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity
title Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity
title_full Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity
title_fullStr Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity
title_full_unstemmed Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity
title_short Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo A Biomarker for LDH Activity
title_sort lactate chemical exchange saturation transfer (latest) imaging in vivo a biomarker for ldh activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726389/
https://www.ncbi.nlm.nih.gov/pubmed/26794265
http://dx.doi.org/10.1038/srep19517
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