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A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice
Transgenic Tg2576 mice overexpressing human amyloid precursor protein (hAPP) are a widely used Alzheimer’s disease (AD) mouse model to evaluate treatment effects on amyloid beta (Aβ) pathology and cognition. Tg2576 mice on a B6;SJL background strain carry a recessive rd1 mutation that leads to early...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726499/ https://www.ncbi.nlm.nih.gov/pubmed/26808326 http://dx.doi.org/10.1371/journal.pone.0147733 |
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author | Wolf, Andrea Bauer, Björn Abner, Erin L. Ashkenazy-Frolinger, Tal Hartz, Anika M. S. |
author_facet | Wolf, Andrea Bauer, Björn Abner, Erin L. Ashkenazy-Frolinger, Tal Hartz, Anika M. S. |
author_sort | Wolf, Andrea |
collection | PubMed |
description | Transgenic Tg2576 mice overexpressing human amyloid precursor protein (hAPP) are a widely used Alzheimer’s disease (AD) mouse model to evaluate treatment effects on amyloid beta (Aβ) pathology and cognition. Tg2576 mice on a B6;SJL background strain carry a recessive rd1 mutation that leads to early retinal degeneration and visual impairment in homozygous carriers. This can impair performance in behavioral tests that rely on visual cues, and thus, affect study results. Therefore, B6;SJL/Tg2576 mice were systematically backcrossed with 129S6/SvEvTac mice resulting in 129S6/Tg2576 mice that lack the rd1 mutation. 129S6/Tg2576 mice do not develop retinal degeneration but still show Aβ accumulation in the brain that is comparable to the original B6;SJL/Tg2576 mouse. However, comprehensive studies on cognitive decline in 129S6/Tg2576 mice are limited. In this study, we used two dementia mouse models on a 129S6 background—scopolamine-treated 129S6/SvEvTac mice (3–5 month-old) and transgenic 129S6/Tg2576 mice (11–13 month-old)–to establish a behavioral test battery for assessing learning and memory. The test battery consisted of five tests to evaluate different aspects of cognitive impairment: a Y-Maze forced alternation task, a novel object recognition test, the Morris water maze, the radial arm water maze, and a Y-maze spontaneous alternation task. We first established this behavioral test battery with the scopolamine-induced dementia model using 129S6/SvEvTac mice and then evaluated 129S6/Tg2576 mice using the same testing protocol. Both models showed distinctive patterns of cognitive impairment. Together, the non-invasive behavioral test battery presented here allows detecting cognitive impairment in scopolamine-treated 129S6/SvEvTac mice and in transgenic 129S6/Tg2576 mice. Due to the modular nature of this test battery, more behavioral tests, e.g. invasive assays to gain additional cognitive information, can easily be added. |
format | Online Article Text |
id | pubmed-4726499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47264992016-02-03 A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice Wolf, Andrea Bauer, Björn Abner, Erin L. Ashkenazy-Frolinger, Tal Hartz, Anika M. S. PLoS One Research Article Transgenic Tg2576 mice overexpressing human amyloid precursor protein (hAPP) are a widely used Alzheimer’s disease (AD) mouse model to evaluate treatment effects on amyloid beta (Aβ) pathology and cognition. Tg2576 mice on a B6;SJL background strain carry a recessive rd1 mutation that leads to early retinal degeneration and visual impairment in homozygous carriers. This can impair performance in behavioral tests that rely on visual cues, and thus, affect study results. Therefore, B6;SJL/Tg2576 mice were systematically backcrossed with 129S6/SvEvTac mice resulting in 129S6/Tg2576 mice that lack the rd1 mutation. 129S6/Tg2576 mice do not develop retinal degeneration but still show Aβ accumulation in the brain that is comparable to the original B6;SJL/Tg2576 mouse. However, comprehensive studies on cognitive decline in 129S6/Tg2576 mice are limited. In this study, we used two dementia mouse models on a 129S6 background—scopolamine-treated 129S6/SvEvTac mice (3–5 month-old) and transgenic 129S6/Tg2576 mice (11–13 month-old)–to establish a behavioral test battery for assessing learning and memory. The test battery consisted of five tests to evaluate different aspects of cognitive impairment: a Y-Maze forced alternation task, a novel object recognition test, the Morris water maze, the radial arm water maze, and a Y-maze spontaneous alternation task. We first established this behavioral test battery with the scopolamine-induced dementia model using 129S6/SvEvTac mice and then evaluated 129S6/Tg2576 mice using the same testing protocol. Both models showed distinctive patterns of cognitive impairment. Together, the non-invasive behavioral test battery presented here allows detecting cognitive impairment in scopolamine-treated 129S6/SvEvTac mice and in transgenic 129S6/Tg2576 mice. Due to the modular nature of this test battery, more behavioral tests, e.g. invasive assays to gain additional cognitive information, can easily be added. Public Library of Science 2016-01-25 /pmc/articles/PMC4726499/ /pubmed/26808326 http://dx.doi.org/10.1371/journal.pone.0147733 Text en © 2016 Wolf et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wolf, Andrea Bauer, Björn Abner, Erin L. Ashkenazy-Frolinger, Tal Hartz, Anika M. S. A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice |
title | A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice |
title_full | A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice |
title_fullStr | A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice |
title_full_unstemmed | A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice |
title_short | A Comprehensive Behavioral Test Battery to Assess Learning and Memory in 129S6/Tg2576 Mice |
title_sort | comprehensive behavioral test battery to assess learning and memory in 129s6/tg2576 mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726499/ https://www.ncbi.nlm.nih.gov/pubmed/26808326 http://dx.doi.org/10.1371/journal.pone.0147733 |
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