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Utilization of Site-Specific Recombination in Biopharmaceutical Production
Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improv...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pasteur Institute of Iran
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726886/ https://www.ncbi.nlm.nih.gov/pubmed/26602035 http://dx.doi.org/10.7508/ibj.2016.02.001 |
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author | Ahmadi, Maryam Damavandi, Narges Akbari, Mohammad Reza Davami, Fatemeh |
author_facet | Ahmadi, Maryam Damavandi, Narges Akbari, Mohammad Reza Davami, Fatemeh |
author_sort | Ahmadi, Maryam |
collection | PubMed |
description | Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells; therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons. |
format | Online Article Text |
id | pubmed-4726886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Pasteur Institute of Iran |
record_format | MEDLINE/PubMed |
spelling | pubmed-47268862016-04-01 Utilization of Site-Specific Recombination in Biopharmaceutical Production Ahmadi, Maryam Damavandi, Narges Akbari, Mohammad Reza Davami, Fatemeh Iran Biomed J Review Article Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells; therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons. Pasteur Institute of Iran 2016-04 /pmc/articles/PMC4726886/ /pubmed/26602035 http://dx.doi.org/10.7508/ibj.2016.02.001 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Ahmadi, Maryam Damavandi, Narges Akbari, Mohammad Reza Davami, Fatemeh Utilization of Site-Specific Recombination in Biopharmaceutical Production |
title | Utilization of Site-Specific Recombination in Biopharmaceutical Production |
title_full | Utilization of Site-Specific Recombination in Biopharmaceutical Production |
title_fullStr | Utilization of Site-Specific Recombination in Biopharmaceutical Production |
title_full_unstemmed | Utilization of Site-Specific Recombination in Biopharmaceutical Production |
title_short | Utilization of Site-Specific Recombination in Biopharmaceutical Production |
title_sort | utilization of site-specific recombination in biopharmaceutical production |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726886/ https://www.ncbi.nlm.nih.gov/pubmed/26602035 http://dx.doi.org/10.7508/ibj.2016.02.001 |
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