Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

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The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA vi...

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Autores principales: Almazán, Fernando, Márquez-Jurado, Silvia, Nogales, Aitor, Enjuanes, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726977/
https://www.ncbi.nlm.nih.gov/pubmed/25720478
http://dx.doi.org/10.1007/978-1-4939-2438-7_13
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author Almazán, Fernando
Márquez-Jurado, Silvia
Nogales, Aitor
Enjuanes, Luis
author_facet Almazán, Fernando
Márquez-Jurado, Silvia
Nogales, Aitor
Enjuanes, Luis
author_sort Almazán, Fernando
collection PubMed
description The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.
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spelling pubmed-47269772016-01-26 Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes Almazán, Fernando Márquez-Jurado, Silvia Nogales, Aitor Enjuanes, Luis Coronaviruses Article The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model. 2014-12-18 /pmc/articles/PMC4726977/ /pubmed/25720478 http://dx.doi.org/10.1007/978-1-4939-2438-7_13 Text en © Springer Science+Business Media New York 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Almazán, Fernando
Márquez-Jurado, Silvia
Nogales, Aitor
Enjuanes, Luis
Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_full Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_fullStr Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_full_unstemmed Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_short Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_sort engineering infectious cdnas of coronavirus as bacterial artificial chromosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726977/
https://www.ncbi.nlm.nih.gov/pubmed/25720478
http://dx.doi.org/10.1007/978-1-4939-2438-7_13
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