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miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6

MicroRNAs (miRNAs) are small non-coding RNAs that participate in several cellular functions and tumour progression. A previous microarray study demonstrated that miR-135b is downregulated in prostate cancer (PCa) cells, but the role and molecular mechanism of miR-135b in the regulation of tumour met...

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Autores principales: WANG, NING, TAO, LIANGJUN, ZHONG, HUAN, ZHAO, SIHAI, YU, YING, YU, BIN, CHEN, XIAONONG, GAO, JIANGUO, WANG, RONGJIANG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727074/
https://www.ncbi.nlm.nih.gov/pubmed/26870245
http://dx.doi.org/10.3892/ol.2015.3970
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author WANG, NING
TAO, LIANGJUN
ZHONG, HUAN
ZHAO, SIHAI
YU, YING
YU, BIN
CHEN, XIAONONG
GAO, JIANGUO
WANG, RONGJIANG
author_facet WANG, NING
TAO, LIANGJUN
ZHONG, HUAN
ZHAO, SIHAI
YU, YING
YU, BIN
CHEN, XIAONONG
GAO, JIANGUO
WANG, RONGJIANG
author_sort WANG, NING
collection PubMed
description MicroRNAs (miRNAs) are small non-coding RNAs that participate in several cellular functions and tumour progression. A previous microarray study demonstrated that miR-135b is downregulated in prostate cancer (PCa) cells, but the role and molecular mechanism of miR-135b in the regulation of tumour metastasis remain to be elucidated. In the present study, significant downregulation of miR-135b in PCa tissues, compared with noncancerous tissues, was detected by reverse transcription-quantitative polymerase chain reaction. Furthermore, the expression of miR-135b was demonstrated to be associated with the pathological stage and the levels of total and free prostate-specific antigen (PSA) in PCa cells. In addition, signal transducer and activator of transcription 6 (STAT6) was identified as a target of miR-135b in PCa cells by luciferase activity and western blot assays. The upregulation of miR-135b in PCa cells led to reduced expression of STAT6 in the cytoplasm and nucleus of these cells, while the overexpression of miR-135b and knockdown of STAT6 were able to inhibit the migration and invasion abilities of PCa cells in vitro. Therefore, the results of the present study indicate that miR-135b suppresses tumour metastasis by targeting STAT6.
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spelling pubmed-47270742016-02-11 miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6 WANG, NING TAO, LIANGJUN ZHONG, HUAN ZHAO, SIHAI YU, YING YU, BIN CHEN, XIAONONG GAO, JIANGUO WANG, RONGJIANG Oncol Lett Articles MicroRNAs (miRNAs) are small non-coding RNAs that participate in several cellular functions and tumour progression. A previous microarray study demonstrated that miR-135b is downregulated in prostate cancer (PCa) cells, but the role and molecular mechanism of miR-135b in the regulation of tumour metastasis remain to be elucidated. In the present study, significant downregulation of miR-135b in PCa tissues, compared with noncancerous tissues, was detected by reverse transcription-quantitative polymerase chain reaction. Furthermore, the expression of miR-135b was demonstrated to be associated with the pathological stage and the levels of total and free prostate-specific antigen (PSA) in PCa cells. In addition, signal transducer and activator of transcription 6 (STAT6) was identified as a target of miR-135b in PCa cells by luciferase activity and western blot assays. The upregulation of miR-135b in PCa cells led to reduced expression of STAT6 in the cytoplasm and nucleus of these cells, while the overexpression of miR-135b and knockdown of STAT6 were able to inhibit the migration and invasion abilities of PCa cells in vitro. Therefore, the results of the present study indicate that miR-135b suppresses tumour metastasis by targeting STAT6. D.A. Spandidos 2016-01 2015-11-25 /pmc/articles/PMC4727074/ /pubmed/26870245 http://dx.doi.org/10.3892/ol.2015.3970 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
WANG, NING
TAO, LIANGJUN
ZHONG, HUAN
ZHAO, SIHAI
YU, YING
YU, BIN
CHEN, XIAONONG
GAO, JIANGUO
WANG, RONGJIANG
miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6
title miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6
title_full miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6
title_fullStr miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6
title_full_unstemmed miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6
title_short miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6
title_sort mir-135b inhibits tumour metastasis in prostate cancer by targeting stat6
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727074/
https://www.ncbi.nlm.nih.gov/pubmed/26870245
http://dx.doi.org/10.3892/ol.2015.3970
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