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PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto de Medicina Tropical
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727131/ https://www.ncbi.nlm.nih.gov/pubmed/27049699 http://dx.doi.org/10.1590/S0036-46652015000600002 |
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author | PAVANELLI, Mariana F. KANESHIMA, Edilson Nobuyoshi UDA, Carla F. COLLI, Cristiane M. FALAVIGNA-GUILHERM, Ana L. GOMES, Mônica L. |
author_facet | PAVANELLI, Mariana F. KANESHIMA, Edilson Nobuyoshi UDA, Carla F. COLLI, Cristiane M. FALAVIGNA-GUILHERM, Ana L. GOMES, Mônica L. |
author_sort | PAVANELLI, Mariana F. |
collection | PubMed |
description | The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastrically, with 100, 500, 1,000, 5,000 and 10,000 Blastocystis sp. vacuolar forms obtained from a mixture of eight human isolates cultured axenically in Jones' medium. After seven, 14, 21, 28 and 60 days of infection, the animals were sacrificed and fragments of the small intestine (duodenum), large intestine, and cecum were subjected to histopathological analysis. Blastocystis sp. triggered an inflammatory response in the different tissues analyzed, with a predominance of mononuclear cells. The parasite was found in the muscular layer of the cecum, showing its invasive character. Larger inocula triggered inflammatory processes earlier (seven days) than smaller ones (from 21 days). We conclude that, in the proposed model, the pathogenicity of Blastocystis sp. isolates that were studied is related to inoculum size and period of infection. |
format | Online Article Text |
id | pubmed-4727131 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-47271312016-01-31 PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION PAVANELLI, Mariana F. KANESHIMA, Edilson Nobuyoshi UDA, Carla F. COLLI, Cristiane M. FALAVIGNA-GUILHERM, Ana L. GOMES, Mônica L. Rev Inst Med Trop Sao Paulo Parasitology The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastrically, with 100, 500, 1,000, 5,000 and 10,000 Blastocystis sp. vacuolar forms obtained from a mixture of eight human isolates cultured axenically in Jones' medium. After seven, 14, 21, 28 and 60 days of infection, the animals were sacrificed and fragments of the small intestine (duodenum), large intestine, and cecum were subjected to histopathological analysis. Blastocystis sp. triggered an inflammatory response in the different tissues analyzed, with a predominance of mononuclear cells. The parasite was found in the muscular layer of the cecum, showing its invasive character. Larger inocula triggered inflammatory processes earlier (seven days) than smaller ones (from 21 days). We conclude that, in the proposed model, the pathogenicity of Blastocystis sp. isolates that were studied is related to inoculum size and period of infection. Instituto de Medicina Tropical 2015 /pmc/articles/PMC4727131/ /pubmed/27049699 http://dx.doi.org/10.1590/S0036-46652015000600002 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Parasitology PAVANELLI, Mariana F. KANESHIMA, Edilson Nobuyoshi UDA, Carla F. COLLI, Cristiane M. FALAVIGNA-GUILHERM, Ana L. GOMES, Mônica L. PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION |
title | PATHOGENICITY OF Blastocystis sp. TO THE
GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF
INFECTION |
title_full | PATHOGENICITY OF Blastocystis sp. TO THE
GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF
INFECTION |
title_fullStr | PATHOGENICITY OF Blastocystis sp. TO THE
GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF
INFECTION |
title_full_unstemmed | PATHOGENICITY OF Blastocystis sp. TO THE
GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF
INFECTION |
title_short | PATHOGENICITY OF Blastocystis sp. TO THE
GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF
INFECTION |
title_sort | pathogenicity of blastocystis sp. to the
gastrointestinal tract of mice: relationship between inoculum size and period of
infection |
topic | Parasitology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727131/ https://www.ncbi.nlm.nih.gov/pubmed/27049699 http://dx.doi.org/10.1590/S0036-46652015000600002 |
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