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PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION

The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastr...

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Autores principales: PAVANELLI, Mariana F., KANESHIMA, Edilson Nobuyoshi, UDA, Carla F., COLLI, Cristiane M., FALAVIGNA-GUILHERM, Ana L., GOMES, Mônica L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727131/
https://www.ncbi.nlm.nih.gov/pubmed/27049699
http://dx.doi.org/10.1590/S0036-46652015000600002
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author PAVANELLI, Mariana F.
KANESHIMA, Edilson Nobuyoshi
UDA, Carla F.
COLLI, Cristiane M.
FALAVIGNA-GUILHERM, Ana L.
GOMES, Mônica L.
author_facet PAVANELLI, Mariana F.
KANESHIMA, Edilson Nobuyoshi
UDA, Carla F.
COLLI, Cristiane M.
FALAVIGNA-GUILHERM, Ana L.
GOMES, Mônica L.
author_sort PAVANELLI, Mariana F.
collection PubMed
description The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastrically, with 100, 500, 1,000, 5,000 and 10,000 Blastocystis sp. vacuolar forms obtained from a mixture of eight human isolates cultured axenically in Jones' medium. After seven, 14, 21, 28 and 60 days of infection, the animals were sacrificed and fragments of the small intestine (duodenum), large intestine, and cecum were subjected to histopathological analysis. Blastocystis sp. triggered an inflammatory response in the different tissues analyzed, with a predominance of mononuclear cells. The parasite was found in the muscular layer of the cecum, showing its invasive character. Larger inocula triggered inflammatory processes earlier (seven days) than smaller ones (from 21 days). We conclude that, in the proposed model, the pathogenicity of Blastocystis sp. isolates that were studied is related to inoculum size and period of infection.
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spelling pubmed-47271312016-01-31 PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION PAVANELLI, Mariana F. KANESHIMA, Edilson Nobuyoshi UDA, Carla F. COLLI, Cristiane M. FALAVIGNA-GUILHERM, Ana L. GOMES, Mônica L. Rev Inst Med Trop Sao Paulo Parasitology The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastrically, with 100, 500, 1,000, 5,000 and 10,000 Blastocystis sp. vacuolar forms obtained from a mixture of eight human isolates cultured axenically in Jones' medium. After seven, 14, 21, 28 and 60 days of infection, the animals were sacrificed and fragments of the small intestine (duodenum), large intestine, and cecum were subjected to histopathological analysis. Blastocystis sp. triggered an inflammatory response in the different tissues analyzed, with a predominance of mononuclear cells. The parasite was found in the muscular layer of the cecum, showing its invasive character. Larger inocula triggered inflammatory processes earlier (seven days) than smaller ones (from 21 days). We conclude that, in the proposed model, the pathogenicity of Blastocystis sp. isolates that were studied is related to inoculum size and period of infection. Instituto de Medicina Tropical 2015 /pmc/articles/PMC4727131/ /pubmed/27049699 http://dx.doi.org/10.1590/S0036-46652015000600002 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Parasitology
PAVANELLI, Mariana F.
KANESHIMA, Edilson Nobuyoshi
UDA, Carla F.
COLLI, Cristiane M.
FALAVIGNA-GUILHERM, Ana L.
GOMES, Mônica L.
PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
title PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
title_full PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
title_fullStr PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
title_full_unstemmed PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
title_short PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION
title_sort pathogenicity of blastocystis sp. to the gastrointestinal tract of mice: relationship between inoculum size and period of infection
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727131/
https://www.ncbi.nlm.nih.gov/pubmed/27049699
http://dx.doi.org/10.1590/S0036-46652015000600002
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