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Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis
BACKGROUND: Water-soluble form of fullerene C(60) is a promising tool for the control of ROS-dependent inflammation including allergic diseases. Anti-inflammatory effects of C(60) (nC(60)) aqueous dispersion were evaluated in the mouse models of atopic dermatitis using subcutaneous (SC) and epicutan...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727272/ https://www.ncbi.nlm.nih.gov/pubmed/26810232 http://dx.doi.org/10.1186/s12951-016-0159-z |
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author | Shershakova, Nadezda Baraboshkina, Elena Andreev, Sergey Purgina, Daria Struchkova, Irina Kamyshnikov, Oleg Nikonova, Alexandra Khaitov, Musa |
author_facet | Shershakova, Nadezda Baraboshkina, Elena Andreev, Sergey Purgina, Daria Struchkova, Irina Kamyshnikov, Oleg Nikonova, Alexandra Khaitov, Musa |
author_sort | Shershakova, Nadezda |
collection | PubMed |
description | BACKGROUND: Water-soluble form of fullerene C(60) is a promising tool for the control of ROS-dependent inflammation including allergic diseases. Anti-inflammatory effects of C(60) (nC(60)) aqueous dispersion were evaluated in the mouse models of atopic dermatitis using subcutaneous (SC) and epicutaneous (EC) applications during 50 days period. A highly stable nC(60) was prepared by exhaustive dialysis of water-organic C(60) solution against water, where the size and ζ-potential of fullerene nanoparticles are about 100 nm and −30 mV, respectively. RESULTS: To induce skin inflammation, female BALB/c mice were EC sensitized with ovalbumin three times during one-weekly exposures. The nC(60) solution was administrated in mice subcutaneously (SC) (0.1 mg/kg) and epicutaneously (EC) (1 mg/kg). Significant suppression of IgE and Th2 cytokines production and a concomitant rise in concentrations of Th1 cytokines were observed in nC(60)-treated groups. In addition, a significant increase in the levels of Foxp3(+) and filaggrin mRNA expression was observed at EC application. Histological examination of skin samples indicated that therapeutic effect was achieved by both EC and SC treatment, but it was more effective with EC. Pronounced reduction of the eosinophil and leukocyte infiltration in treated skin samples was observed. CONCLUSIONS: We suppose that nC60 treatment shifts immune response from Th2 to Th1 and restores to some extent the function of the skin barrier. This approach can be a good alternative to the treatment of allergic and other inflammatory diseases. |
format | Online Article Text |
id | pubmed-4727272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47272722016-01-27 Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis Shershakova, Nadezda Baraboshkina, Elena Andreev, Sergey Purgina, Daria Struchkova, Irina Kamyshnikov, Oleg Nikonova, Alexandra Khaitov, Musa J Nanobiotechnology Research BACKGROUND: Water-soluble form of fullerene C(60) is a promising tool for the control of ROS-dependent inflammation including allergic diseases. Anti-inflammatory effects of C(60) (nC(60)) aqueous dispersion were evaluated in the mouse models of atopic dermatitis using subcutaneous (SC) and epicutaneous (EC) applications during 50 days period. A highly stable nC(60) was prepared by exhaustive dialysis of water-organic C(60) solution against water, where the size and ζ-potential of fullerene nanoparticles are about 100 nm and −30 mV, respectively. RESULTS: To induce skin inflammation, female BALB/c mice were EC sensitized with ovalbumin three times during one-weekly exposures. The nC(60) solution was administrated in mice subcutaneously (SC) (0.1 mg/kg) and epicutaneously (EC) (1 mg/kg). Significant suppression of IgE and Th2 cytokines production and a concomitant rise in concentrations of Th1 cytokines were observed in nC(60)-treated groups. In addition, a significant increase in the levels of Foxp3(+) and filaggrin mRNA expression was observed at EC application. Histological examination of skin samples indicated that therapeutic effect was achieved by both EC and SC treatment, but it was more effective with EC. Pronounced reduction of the eosinophil and leukocyte infiltration in treated skin samples was observed. CONCLUSIONS: We suppose that nC60 treatment shifts immune response from Th2 to Th1 and restores to some extent the function of the skin barrier. This approach can be a good alternative to the treatment of allergic and other inflammatory diseases. BioMed Central 2016-01-25 /pmc/articles/PMC4727272/ /pubmed/26810232 http://dx.doi.org/10.1186/s12951-016-0159-z Text en © Shershakova et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Shershakova, Nadezda Baraboshkina, Elena Andreev, Sergey Purgina, Daria Struchkova, Irina Kamyshnikov, Oleg Nikonova, Alexandra Khaitov, Musa Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis |
title | Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis |
title_full | Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis |
title_fullStr | Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis |
title_full_unstemmed | Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis |
title_short | Anti-inflammatory effect of fullerene C(60) in a mice model of atopic dermatitis |
title_sort | anti-inflammatory effect of fullerene c(60) in a mice model of atopic dermatitis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727272/ https://www.ncbi.nlm.nih.gov/pubmed/26810232 http://dx.doi.org/10.1186/s12951-016-0159-z |
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