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Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli

BACKGROUND: Disulfide bonds are the most common structural, post-translational modification found in proteins. Antibodies contain up to 25 disulfide bonds depending on type, with scFv fragments containing two disulfides and Fab fragments containing five or six disulfide bonds. The production of anti...

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Detalles Bibliográficos
Autores principales:	Gaciarz, Anna, Veijola, Johanna, Uchida, Yuko, Saaranen, Mirva J., Wang, Chunguang, Hörkkö, Sohvi, Ruddock, Lloyd W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727284/ https://www.ncbi.nlm.nih.gov/pubmed/26809624 http://dx.doi.org/10.1186/s12934-016-0419-5

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