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Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy
PURPOSE: We compared corneal backscatter estimated from a Scheimpflug camera with backscatter estimated from a clinical confocal microscope across a wide range of corneal haze. METHODS: A total of 59 corneas from 35 patients with a range of severity of Fuchs' endothelial corneal dystrophy and 1...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727526/ https://www.ncbi.nlm.nih.gov/pubmed/26803798 http://dx.doi.org/10.1167/iovs.15-17657 |
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author | McLaren, Jay W. Wacker, Katrin Kane, Katrina M. Patel, Sanjay V. |
author_facet | McLaren, Jay W. Wacker, Katrin Kane, Katrina M. Patel, Sanjay V. |
author_sort | McLaren, Jay W. |
collection | PubMed |
description | PURPOSE: We compared corneal backscatter estimated from a Scheimpflug camera with backscatter estimated from a clinical confocal microscope across a wide range of corneal haze. METHODS: A total of 59 corneas from 35 patients with a range of severity of Fuchs' endothelial corneal dystrophy and 15 corneas from 9 normal participants were examined using a Scheimpflug camera (Pentacam) and a confocal microscope (ConfoScan 4). The mean image brightness from the anterior 120 μm, midcornea, and posterior 60 μm of the cornea across the central 2 mm recorded by the Scheimpflug camera and analogous regions from the confocal microscope were measured and standardized. Differences between instruments and correlations between backscatter and disease severity were determined by using generalized estimating equation models. RESULTS: Backscatter measured by the two instruments in the anterior and midcornea were correlated (r = 0.67 and 0.43, respectively, P < 0.001), although in the posterior cornea they were not correlated (r = 0.13, P = 0.66). Measured with the Scheimpflug camera, mean backscatter from the anterior and midcornea were greater, whereas backscatter from the posterior cornea was lower (P < 0.001) than that measured by the confocal microscope. Backscatter from the anterior cornea was correlated with disease severity for both instruments (Scheimpflug, r = 0.55, P < 0.001; confocal, r = 0.49, P = 0.003). CONCLUSIONS: The Scheimpflug camera and confocal microscope should not be used interchangeably to measure corneal haze. The ability to detect changes in backscatter with disease severity is superior with the Scheimpflug camera. However, the confocal microscope provides higher resolution of corneal structure. |
format | Online Article Text |
id | pubmed-4727526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-47275262016-07-01 Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy McLaren, Jay W. Wacker, Katrin Kane, Katrina M. Patel, Sanjay V. Invest Ophthalmol Vis Sci Cornea PURPOSE: We compared corneal backscatter estimated from a Scheimpflug camera with backscatter estimated from a clinical confocal microscope across a wide range of corneal haze. METHODS: A total of 59 corneas from 35 patients with a range of severity of Fuchs' endothelial corneal dystrophy and 15 corneas from 9 normal participants were examined using a Scheimpflug camera (Pentacam) and a confocal microscope (ConfoScan 4). The mean image brightness from the anterior 120 μm, midcornea, and posterior 60 μm of the cornea across the central 2 mm recorded by the Scheimpflug camera and analogous regions from the confocal microscope were measured and standardized. Differences between instruments and correlations between backscatter and disease severity were determined by using generalized estimating equation models. RESULTS: Backscatter measured by the two instruments in the anterior and midcornea were correlated (r = 0.67 and 0.43, respectively, P < 0.001), although in the posterior cornea they were not correlated (r = 0.13, P = 0.66). Measured with the Scheimpflug camera, mean backscatter from the anterior and midcornea were greater, whereas backscatter from the posterior cornea was lower (P < 0.001) than that measured by the confocal microscope. Backscatter from the anterior cornea was correlated with disease severity for both instruments (Scheimpflug, r = 0.55, P < 0.001; confocal, r = 0.49, P = 0.003). CONCLUSIONS: The Scheimpflug camera and confocal microscope should not be used interchangeably to measure corneal haze. The ability to detect changes in backscatter with disease severity is superior with the Scheimpflug camera. However, the confocal microscope provides higher resolution of corneal structure. The Association for Research in Vision and Ophthalmology 2016-01-22 2016-01 /pmc/articles/PMC4727526/ /pubmed/26803798 http://dx.doi.org/10.1167/iovs.15-17657 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Cornea McLaren, Jay W. Wacker, Katrin Kane, Katrina M. Patel, Sanjay V. Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy |
title | Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy |
title_full | Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy |
title_fullStr | Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy |
title_full_unstemmed | Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy |
title_short | Measuring Corneal Haze by Using Scheimpflug Photography and Confocal Microscopy |
title_sort | measuring corneal haze by using scheimpflug photography and confocal microscopy |
topic | Cornea |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727526/ https://www.ncbi.nlm.nih.gov/pubmed/26803798 http://dx.doi.org/10.1167/iovs.15-17657 |
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