Improved Automated Radiosynthesis of [(11)C]PBR28

Microglial activation is commonly identified by elevated levels of the 18 kDa translocator protein (TSPO) in response to several inflammatory processes. [(11)C]PBR28 is one of the most promising PET tracers to image TSPO in both human and non-human primates. In this study, we optimized the radiolabe...

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Autores principales: Solingapuram Sai, Kiran Kumar, Gage, Don, Nader, Mike, Mach, Robert H., Mintz, Akiva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Austrian Journal of Pharmaceutical Sciences 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727796/
https://www.ncbi.nlm.nih.gov/pubmed/26839827
http://dx.doi.org/10.3797/scipharm.1505-06
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author Solingapuram Sai, Kiran Kumar
Gage, Don
Nader, Mike
Mach, Robert H.
Mintz, Akiva
author_facet Solingapuram Sai, Kiran Kumar
Gage, Don
Nader, Mike
Mach, Robert H.
Mintz, Akiva
author_sort Solingapuram Sai, Kiran Kumar
collection PubMed
description Microglial activation is commonly identified by elevated levels of the 18 kDa translocator protein (TSPO) in response to several inflammatory processes. [(11)C]PBR28 is one of the most promising PET tracers to image TSPO in both human and non-human primates. In this study, we optimized the radiolabeling procedure of [(11)C]PBR28 for higher radiochemical yield, radiochemical purity, and specific activity, which can be easily translated to any automated module for clinical trials. Time-activity curves (TACs) derived from the dynamic PET imaging of male rhesus monkey brains demonstrated that [(11)C]PBR28 had suitable kinetics with radiotracer accumulation observed in the caudate, putamen, cerebellum, and frontal cortex region.
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spelling pubmed-47277962016-02-02 Improved Automated Radiosynthesis of [(11)C]PBR28 Solingapuram Sai, Kiran Kumar Gage, Don Nader, Mike Mach, Robert H. Mintz, Akiva Sci Pharm Research Article Microglial activation is commonly identified by elevated levels of the 18 kDa translocator protein (TSPO) in response to several inflammatory processes. [(11)C]PBR28 is one of the most promising PET tracers to image TSPO in both human and non-human primates. In this study, we optimized the radiolabeling procedure of [(11)C]PBR28 for higher radiochemical yield, radiochemical purity, and specific activity, which can be easily translated to any automated module for clinical trials. Time-activity curves (TACs) derived from the dynamic PET imaging of male rhesus monkey brains demonstrated that [(11)C]PBR28 had suitable kinetics with radiotracer accumulation observed in the caudate, putamen, cerebellum, and frontal cortex region. The Austrian Journal of Pharmaceutical Sciences 2015 2015-06-19 /pmc/articles/PMC4727796/ /pubmed/26839827 http://dx.doi.org/10.3797/scipharm.1505-06 Text en Copyright: © Solingapuram Sai et al. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Solingapuram Sai, Kiran Kumar
Gage, Don
Nader, Mike
Mach, Robert H.
Mintz, Akiva
Improved Automated Radiosynthesis of [(11)C]PBR28
title Improved Automated Radiosynthesis of [(11)C]PBR28
title_full Improved Automated Radiosynthesis of [(11)C]PBR28
title_fullStr Improved Automated Radiosynthesis of [(11)C]PBR28
title_full_unstemmed Improved Automated Radiosynthesis of [(11)C]PBR28
title_short Improved Automated Radiosynthesis of [(11)C]PBR28
title_sort improved automated radiosynthesis of [(11)c]pbr28
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727796/
https://www.ncbi.nlm.nih.gov/pubmed/26839827
http://dx.doi.org/10.3797/scipharm.1505-06
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