Cargando…

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system

The CRISPR-Cas9 system has recently emerged as a versatile tool for biological and medical research. In this system, a single guide RNA (sgRNA) directs the endonuclease Cas9 to a targeted DNA sequence for site-specific manipulation. In addition to this targeting function, the sgRNA has also been sho...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Xiaoxi, Homma, Ayaka, Sayadi, Jamasb, Yang, Shu, Ohashi, Jun, Takumi, Toru
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728555/ https://www.ncbi.nlm.nih.gov/pubmed/26813419 http://dx.doi.org/10.1038/srep19675

Ejemplares similares

A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency
por: Jung, Soo Bin, et al.
Publicado: (2021)

PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues
por: Veeneman, Brendan, et al.
Publicado: (2020)

Programmable RNA recognition and cleavage by CRISPR/Cas9
por: O’Connell, Mitchell R., et al.
Publicado: (2014)

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
por: Ramanan, Vyas, et al.
Publicado: (2015)

BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
por: Zarate, Oscar A., et al.
Publicado: (2022)

Structural insights into DNA cleavage activation of CRISPR-Cas9 system
por: Huai, Cong, et al.
Publicado: (2017)

Conformational control of DNA target cleavage by CRISPR–Cas9
por: Sternberg, Samuel H., et al.
Publicado: (2015)

Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
por: Yarrington, Robert M., et al.
Publicado: (2018)

Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
por: Das, Jutan, et al.
Publicado: (2023)

Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus
por: Yang, Ying-jie, et al.
Publicado: (2017)

Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells
por: Tang, Lianchao, et al.
Publicado: (2019)

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage
por: Sun, Jichao, et al.
Publicado: (2019)

Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
por: Cencic, Regina, et al.
Publicado: (2014)

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
por: Dagdas, Yavuz S., et al.
Publicado: (2017)

Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage
por: Nahmad, A.D., et al.
Publicado: (2022)

Element coding based accurate evaluation of CRISPR/Cas9 initial cleavage
por: Hu, Jianyu, et al.
Publicado: (2021)

Quantitative evaluation of incomplete preweaning lethality in mice by using the CRISPR/Cas9 system
por: Nakamura, Takumi, et al.
Publicado: (2018)

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
por: Swartjes, Thomas, et al.
Publicado: (2020)

Exome sequencing in the knockin mice generated using the CRISPR/Cas system
por: Nakajima, Kazuo, et al.
Publicado: (2016)

A CRISPR/Cas9 Cleavage System for Capturing Fungal Secondary Metabolite Gene Clusters
por: Xu, Xinran, et al.
Publicado: (2021)

A machine learning approach for predicting CRISPR-Cas9 cleavage efficiencies and patterns underlying its mechanism of action
por: Abadi, Shiran, et al.
Publicado: (2017)

Prediction of CRISPR/Cas9 single guide RNA cleavage efficiency and specificity by attention-based convolutional neural networks
por: Zhang, Guishan, et al.
Publicado: (2021)

FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity
por: Cui, Zifeng, et al.
Publicado: (2022)

Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
por: Martin, Francisco, et al.
Publicado: (2016)

piCRISPR: Physically informed deep learning models for CRISPR/Cas9 off-target cleavage prediction
por: Störtz, Florian, et al.
Publicado: (2023)

Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9
por: Okada, Keita, et al.
Publicado: (2022)

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System
por: Ni, Wei, et al.
Publicado: (2014)

Hybrid Multitask Learning Reveals Sequence Features Driving Specificity in the CRISPR/Cas9 System
por: Vora, Dhvani Sandip, et al.
Publicado: (2023)

Efficient Mitochondrial Genome Editing by CRISPR/Cas9
por: Jo, Areum, et al.
Publicado: (2015)

High Efficiency Targeting of Non-coding Sequences Using CRISPR/Cas9 System in Tilapia
por: Li, Minghui, et al.
Publicado: (2018)

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
por: Zhang, Yilan, et al.
Publicado: (2014)

Permanent Inactivation of HBV Genomes by CRISPR/Cas9-Mediated Non-cleavage Base Editing
por: Yang, Yu-Chan, et al.
Publicado: (2020)

Cleavage of Phage DNA by the Streptococcus thermophilus CRISPR3-Cas System
por: Magadán, Alfonso H., et al.
Publicado: (2012)

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
por: Bassett, Andrew R., et al.
Publicado: (2013)

In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
por: Liu, Yunkun, et al.
Publicado: (2015)

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
por: Bassett, Andrew R., et al.
Publicado: (2014)

Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
por: Mallett, Darren R., et al.
Publicado: (2019)

Application of CRISPR/Cas9 System for Efficient Gene Editing in Peanut
por: Neelakandan, Anjanasree K., et al.
Publicado: (2022)

The effect of histone deacetylase inhibitors on the efficiency of the CRISPR/Cas9 system
por: Björnson, Ymer, et al.
Publicado: (2023)

Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
por: Zhang, Jian-Ping, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_9l4qffsu8cd37vm96s246ne9gq