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TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner

The promyelocytic leukemia protein (PML) is the main structural component of the nuclear matrix structures termed nuclear domain 10 (ND10) or PML nuclear bodies (PML-NBs). PML and ND10 structures have been shown to mediate an intrinsic immune response against a variety of different viruses. Their ro...

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Autores principales: Kahle, Tanja, Volkmann, Bianca, Eissmann, Kristin, Herrmann, Alexandra, Schmitt, Sven, Wittmann, Sabine, Merkel, Laura, Reuter, Nina, Stamminger, Thomas, Gramberg, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728562/
https://www.ncbi.nlm.nih.gov/pubmed/26703718
http://dx.doi.org/10.3390/v8010002
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author Kahle, Tanja
Volkmann, Bianca
Eissmann, Kristin
Herrmann, Alexandra
Schmitt, Sven
Wittmann, Sabine
Merkel, Laura
Reuter, Nina
Stamminger, Thomas
Gramberg, Thomas
author_facet Kahle, Tanja
Volkmann, Bianca
Eissmann, Kristin
Herrmann, Alexandra
Schmitt, Sven
Wittmann, Sabine
Merkel, Laura
Reuter, Nina
Stamminger, Thomas
Gramberg, Thomas
author_sort Kahle, Tanja
collection PubMed
description The promyelocytic leukemia protein (PML) is the main structural component of the nuclear matrix structures termed nuclear domain 10 (ND10) or PML nuclear bodies (PML-NBs). PML and ND10 structures have been shown to mediate an intrinsic immune response against a variety of different viruses. Their role during retroviral replication, however, is still controversially discussed. In this study, we analyzed the role of PML and the ND10 components Daxx and Sp100 during retroviral replication in different cell types. Using cell lines exhibiting a shRNA-mediated knockdown, we found that PML, but not Daxx or Sp100, inhibits HIV and other retroviruses in a cell type-dependent manner. The PML-mediated block to retroviral infection was active in primary human fibroblasts and murine embryonic fibroblasts but absent from T cells and myeloid cell lines. Quantitative PCR analysis of HIV cDNA in infected cells revealed that PML restricts infection at the level of reverse transcription. Our findings shed light on the controversial role of PML during retroviral infection and show that PML contributes to the intrinsic restriction of retroviral infections in a cell type-dependent manner.
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spelling pubmed-47285622016-02-08 TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner Kahle, Tanja Volkmann, Bianca Eissmann, Kristin Herrmann, Alexandra Schmitt, Sven Wittmann, Sabine Merkel, Laura Reuter, Nina Stamminger, Thomas Gramberg, Thomas Viruses Article The promyelocytic leukemia protein (PML) is the main structural component of the nuclear matrix structures termed nuclear domain 10 (ND10) or PML nuclear bodies (PML-NBs). PML and ND10 structures have been shown to mediate an intrinsic immune response against a variety of different viruses. Their role during retroviral replication, however, is still controversially discussed. In this study, we analyzed the role of PML and the ND10 components Daxx and Sp100 during retroviral replication in different cell types. Using cell lines exhibiting a shRNA-mediated knockdown, we found that PML, but not Daxx or Sp100, inhibits HIV and other retroviruses in a cell type-dependent manner. The PML-mediated block to retroviral infection was active in primary human fibroblasts and murine embryonic fibroblasts but absent from T cells and myeloid cell lines. Quantitative PCR analysis of HIV cDNA in infected cells revealed that PML restricts infection at the level of reverse transcription. Our findings shed light on the controversial role of PML during retroviral infection and show that PML contributes to the intrinsic restriction of retroviral infections in a cell type-dependent manner. MDPI 2015-12-23 /pmc/articles/PMC4728562/ /pubmed/26703718 http://dx.doi.org/10.3390/v8010002 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kahle, Tanja
Volkmann, Bianca
Eissmann, Kristin
Herrmann, Alexandra
Schmitt, Sven
Wittmann, Sabine
Merkel, Laura
Reuter, Nina
Stamminger, Thomas
Gramberg, Thomas
TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner
title TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner
title_full TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner
title_fullStr TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner
title_full_unstemmed TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner
title_short TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner
title_sort trim19/pml restricts hiv infection in a cell type-dependent manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728562/
https://www.ncbi.nlm.nih.gov/pubmed/26703718
http://dx.doi.org/10.3390/v8010002
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