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Wood reinforcement of poplar by rice NAC transcription factor

Lignocellulose, composed of cellulose, hemicellulose, and lignin, in the secondary cell wall constitutes wood and is the most abundant form of biomass on Earth. Enhancement of wood accumulation may be an effective strategy to increase biomass as well as wood strength, but currently only limited rese...

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Autores principales: Sakamoto, Shingo, Takata, Naoki, Oshima, Yoshimi, Yoshida, Kouki, Taniguchi, Toru, Mitsuda, Nobutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728686/
https://www.ncbi.nlm.nih.gov/pubmed/26812961
http://dx.doi.org/10.1038/srep19925
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author Sakamoto, Shingo
Takata, Naoki
Oshima, Yoshimi
Yoshida, Kouki
Taniguchi, Toru
Mitsuda, Nobutaka
author_facet Sakamoto, Shingo
Takata, Naoki
Oshima, Yoshimi
Yoshida, Kouki
Taniguchi, Toru
Mitsuda, Nobutaka
author_sort Sakamoto, Shingo
collection PubMed
description Lignocellulose, composed of cellulose, hemicellulose, and lignin, in the secondary cell wall constitutes wood and is the most abundant form of biomass on Earth. Enhancement of wood accumulation may be an effective strategy to increase biomass as well as wood strength, but currently only limited research has been undertaken. Here, we demonstrated that OsSWN1, the orthologue of the rice NAC Secondary-wall Thickening factor (NST) transcription factor, effectively enhanced secondary cell wall formation in the Arabidopsis inflorescence stem and poplar (Populus tremula×Populus tremuloides) stem when expressed by the Arabidopsis NST3 promoter. Interestingly, in transgenic Arabidopsis and poplar, ectopic secondary cell wall deposition in the pith area was observed in addition to densification of the secondary cell wall in fiber cells. The cell wall content or density of the stem increased on average by up to 38% and 39% in Arabidopsis and poplar, respectively, without causing growth inhibition. As a result, physical strength of the stem increased by up to 57% in poplar. Collectively, these data suggest that the reinforcement of wood by NST3pro:OsSWN1 is a promising strategy to enhance wood-biomass production in dicotyledonous plant species.
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spelling pubmed-47286862016-02-01 Wood reinforcement of poplar by rice NAC transcription factor Sakamoto, Shingo Takata, Naoki Oshima, Yoshimi Yoshida, Kouki Taniguchi, Toru Mitsuda, Nobutaka Sci Rep Article Lignocellulose, composed of cellulose, hemicellulose, and lignin, in the secondary cell wall constitutes wood and is the most abundant form of biomass on Earth. Enhancement of wood accumulation may be an effective strategy to increase biomass as well as wood strength, but currently only limited research has been undertaken. Here, we demonstrated that OsSWN1, the orthologue of the rice NAC Secondary-wall Thickening factor (NST) transcription factor, effectively enhanced secondary cell wall formation in the Arabidopsis inflorescence stem and poplar (Populus tremula×Populus tremuloides) stem when expressed by the Arabidopsis NST3 promoter. Interestingly, in transgenic Arabidopsis and poplar, ectopic secondary cell wall deposition in the pith area was observed in addition to densification of the secondary cell wall in fiber cells. The cell wall content or density of the stem increased on average by up to 38% and 39% in Arabidopsis and poplar, respectively, without causing growth inhibition. As a result, physical strength of the stem increased by up to 57% in poplar. Collectively, these data suggest that the reinforcement of wood by NST3pro:OsSWN1 is a promising strategy to enhance wood-biomass production in dicotyledonous plant species. Nature Publishing Group 2016-01-27 /pmc/articles/PMC4728686/ /pubmed/26812961 http://dx.doi.org/10.1038/srep19925 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sakamoto, Shingo
Takata, Naoki
Oshima, Yoshimi
Yoshida, Kouki
Taniguchi, Toru
Mitsuda, Nobutaka
Wood reinforcement of poplar by rice NAC transcription factor
title Wood reinforcement of poplar by rice NAC transcription factor
title_full Wood reinforcement of poplar by rice NAC transcription factor
title_fullStr Wood reinforcement of poplar by rice NAC transcription factor
title_full_unstemmed Wood reinforcement of poplar by rice NAC transcription factor
title_short Wood reinforcement of poplar by rice NAC transcription factor
title_sort wood reinforcement of poplar by rice nac transcription factor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728686/
https://www.ncbi.nlm.nih.gov/pubmed/26812961
http://dx.doi.org/10.1038/srep19925
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