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Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran
BACKGROUND: Since the 1990s, influenza A viruses of the H9N2 subtype have been causing infections in the poultry population around the globe. This influenza subtype is widely circulating in poultry and human cases of AI H9N2 have been sporadically reported in countries where this virus is endemic in...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728806/ https://www.ncbi.nlm.nih.gov/pubmed/26817813 http://dx.doi.org/10.1186/s12985-016-0472-z |
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author | Heidari, A. Mancin, M. Nili, H. Pourghanbari, G. H. Lankarani, K. B. Leardini, S. Cattoli, G. Monne, I. Piccirillo, A. |
author_facet | Heidari, A. Mancin, M. Nili, H. Pourghanbari, G. H. Lankarani, K. B. Leardini, S. Cattoli, G. Monne, I. Piccirillo, A. |
author_sort | Heidari, A. |
collection | PubMed |
description | BACKGROUND: Since the 1990s, influenza A viruses of the H9N2 subtype have been causing infections in the poultry population around the globe. This influenza subtype is widely circulating in poultry and human cases of AI H9N2 have been sporadically reported in countries where this virus is endemic in domestic birds. The wide circulation of H9N2 viruses throughout Europe and Asia along with their ability to cause direct infection in mammals and humans, raises public health concerns. H9N2 AI was reported for the first time in Iran in 1998 and at present it is endemic in poultry. This study was carried out to evaluate the exposure to H9N2 AI viruses among poultry workers from the Fars province. METHODS: 100 poultry workers and 100 healthy individuals with no professional exposure to poultry took part in this study. Serum samples were tested for antibodies against two distinct H9N2 avian influenza viruses, which showed different phylogenetic clustering and important molecular differences, such as at the amino acid (aa) position 226 (Q/L) (H3 numbering), using haemagglutination inhibition (HI) and microneutralization (MN) assays. RESULTS: Results showed that 17 % of the poultry workers were positive for the A/chicken/Iran/10VIR/854-5/2008 virus in MN test and 12 % in HI test using the titer ≥40 as positive cut-off value. Only 2 % of the poultry workers were positive for the A/chicken/Iran/12VIR/9630/1998 virus. Seroprevalence of non exposed individuals for both H9N2 strains was below 3 % by both tests. Statistical analyses models showed that exposure to poultry significantly increases the risk of infection with H9N2 virus. CONCLUSIONS: The results have demonstrated that exposure to avian H9N2 viruses had occurred among poultry workers in the Fars province of Iran. Continuous surveillance programmes should be implemented to monitor the presence of avian influenza infections in humans and to evaluate their potential threat to poultry workers and public health. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0472-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4728806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47288062016-01-28 Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran Heidari, A. Mancin, M. Nili, H. Pourghanbari, G. H. Lankarani, K. B. Leardini, S. Cattoli, G. Monne, I. Piccirillo, A. Virol J Research BACKGROUND: Since the 1990s, influenza A viruses of the H9N2 subtype have been causing infections in the poultry population around the globe. This influenza subtype is widely circulating in poultry and human cases of AI H9N2 have been sporadically reported in countries where this virus is endemic in domestic birds. The wide circulation of H9N2 viruses throughout Europe and Asia along with their ability to cause direct infection in mammals and humans, raises public health concerns. H9N2 AI was reported for the first time in Iran in 1998 and at present it is endemic in poultry. This study was carried out to evaluate the exposure to H9N2 AI viruses among poultry workers from the Fars province. METHODS: 100 poultry workers and 100 healthy individuals with no professional exposure to poultry took part in this study. Serum samples were tested for antibodies against two distinct H9N2 avian influenza viruses, which showed different phylogenetic clustering and important molecular differences, such as at the amino acid (aa) position 226 (Q/L) (H3 numbering), using haemagglutination inhibition (HI) and microneutralization (MN) assays. RESULTS: Results showed that 17 % of the poultry workers were positive for the A/chicken/Iran/10VIR/854-5/2008 virus in MN test and 12 % in HI test using the titer ≥40 as positive cut-off value. Only 2 % of the poultry workers were positive for the A/chicken/Iran/12VIR/9630/1998 virus. Seroprevalence of non exposed individuals for both H9N2 strains was below 3 % by both tests. Statistical analyses models showed that exposure to poultry significantly increases the risk of infection with H9N2 virus. CONCLUSIONS: The results have demonstrated that exposure to avian H9N2 viruses had occurred among poultry workers in the Fars province of Iran. Continuous surveillance programmes should be implemented to monitor the presence of avian influenza infections in humans and to evaluate their potential threat to poultry workers and public health. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0472-z) contains supplementary material, which is available to authorized users. BioMed Central 2016-01-27 /pmc/articles/PMC4728806/ /pubmed/26817813 http://dx.doi.org/10.1186/s12985-016-0472-z Text en © Heidari et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Heidari, A. Mancin, M. Nili, H. Pourghanbari, G. H. Lankarani, K. B. Leardini, S. Cattoli, G. Monne, I. Piccirillo, A. Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran |
title | Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran |
title_full | Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran |
title_fullStr | Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran |
title_full_unstemmed | Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran |
title_short | Serological evidence of H9N2 avian influenza virus exposure among poultry workers from Fars province of Iran |
title_sort | serological evidence of h9n2 avian influenza virus exposure among poultry workers from fars province of iran |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728806/ https://www.ncbi.nlm.nih.gov/pubmed/26817813 http://dx.doi.org/10.1186/s12985-016-0472-z |
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