Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables the Discovery of Structural Features Correlated with Kinetic Constants

The use of computational modeling algorithms to guide the design of novel enzyme catalysts is a rapidly growing field. Force-field based methods have now been used to engineer both enzyme specificity and activity. However, the proportion of designed mutants with the intended function is often less t...

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Detalles Bibliográficos
Autores principales: Carlin, Dylan Alexander, Caster, Ryan W., Wang, Xiaokang, Betzenderfer, Stephanie A., Chen, Claire X., Duong, Veasna M., Ryklansky, Carolina V., Alpekin, Alp, Beaumont, Nathan, Kapoor, Harshul, Kim, Nicole, Mohabbot, Hosna, Pang, Boyu, Teel, Rachel, Whithaus, Lillian, Tagkopoulos, Ilias, Siegel, Justin B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729467/
https://www.ncbi.nlm.nih.gov/pubmed/26815142
http://dx.doi.org/10.1371/journal.pone.0147596

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