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Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells

A salivary proline-rich peptide of 1932 Da showed a dose-dependent antagonistic effect on the cytosolic Ca(2+) mobilization induced by progesterone in a tongue squamous carcinoma cell line. Structure-activity studies showed that the activity of the peptide resides in the C-terminal region characteri...

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Autores principales: Palmerini, Carlo Alberto, Mazzoni, Michela, Radicioni, Giorgia, Marzano, Valeria, Granieri, Letizia, Iavarone, Federica, Longhi, Renato, Messana, Irene, Cabras, Tiziana, Sanna, Maria Teresa, Castagnola, Massimo, Vitali, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729474/
https://www.ncbi.nlm.nih.gov/pubmed/26814504
http://dx.doi.org/10.1371/journal.pone.0147925
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author Palmerini, Carlo Alberto
Mazzoni, Michela
Radicioni, Giorgia
Marzano, Valeria
Granieri, Letizia
Iavarone, Federica
Longhi, Renato
Messana, Irene
Cabras, Tiziana
Sanna, Maria Teresa
Castagnola, Massimo
Vitali, Alberto
author_facet Palmerini, Carlo Alberto
Mazzoni, Michela
Radicioni, Giorgia
Marzano, Valeria
Granieri, Letizia
Iavarone, Federica
Longhi, Renato
Messana, Irene
Cabras, Tiziana
Sanna, Maria Teresa
Castagnola, Massimo
Vitali, Alberto
author_sort Palmerini, Carlo Alberto
collection PubMed
description A salivary proline-rich peptide of 1932 Da showed a dose-dependent antagonistic effect on the cytosolic Ca(2+) mobilization induced by progesterone in a tongue squamous carcinoma cell line. Structure-activity studies showed that the activity of the peptide resides in the C-terminal region characterized by a proline stretch flanked by basic residues. Furthermore, lack of activity of the retro-inverso peptide analogue suggested the involvement of stereospecific recognition. Mass spectrometry-based shotgun analysis, combined with Western blotting tests and biochemical data obtained with the Progesterone Receptor Membrane Component 1 (PGRMC1) inhibitor AG205, showed strong evidence that p1932 performs its modulatory action through an interaction with the progesterone receptor PGRMC1, which is predominantly expressed in this cell line and, clearly, plays a role in progesterone induced Ca(2+) response. Thus, our results point to p1932 as a modulator of the transduction signal pathway mediated by this protein and, given a well-established involvement of PGRMC1 in tumorigenesis, highlight a possible therapeutic potential of p1932 for the treatment of oral cancer.
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spelling pubmed-47294742016-02-04 Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells Palmerini, Carlo Alberto Mazzoni, Michela Radicioni, Giorgia Marzano, Valeria Granieri, Letizia Iavarone, Federica Longhi, Renato Messana, Irene Cabras, Tiziana Sanna, Maria Teresa Castagnola, Massimo Vitali, Alberto PLoS One Research Article A salivary proline-rich peptide of 1932 Da showed a dose-dependent antagonistic effect on the cytosolic Ca(2+) mobilization induced by progesterone in a tongue squamous carcinoma cell line. Structure-activity studies showed that the activity of the peptide resides in the C-terminal region characterized by a proline stretch flanked by basic residues. Furthermore, lack of activity of the retro-inverso peptide analogue suggested the involvement of stereospecific recognition. Mass spectrometry-based shotgun analysis, combined with Western blotting tests and biochemical data obtained with the Progesterone Receptor Membrane Component 1 (PGRMC1) inhibitor AG205, showed strong evidence that p1932 performs its modulatory action through an interaction with the progesterone receptor PGRMC1, which is predominantly expressed in this cell line and, clearly, plays a role in progesterone induced Ca(2+) response. Thus, our results point to p1932 as a modulator of the transduction signal pathway mediated by this protein and, given a well-established involvement of PGRMC1 in tumorigenesis, highlight a possible therapeutic potential of p1932 for the treatment of oral cancer. Public Library of Science 2016-01-27 /pmc/articles/PMC4729474/ /pubmed/26814504 http://dx.doi.org/10.1371/journal.pone.0147925 Text en © 2016 Palmerini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Palmerini, Carlo Alberto
Mazzoni, Michela
Radicioni, Giorgia
Marzano, Valeria
Granieri, Letizia
Iavarone, Federica
Longhi, Renato
Messana, Irene
Cabras, Tiziana
Sanna, Maria Teresa
Castagnola, Massimo
Vitali, Alberto
Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells
title Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells
title_full Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells
title_fullStr Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells
title_full_unstemmed Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells
title_short Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca(2+) Mobilization Induced by Progesterone in Oral Squamous Cancer Cells
title_sort antagonistic effect of a salivary proline-rich peptide on the cytosolic ca(2+) mobilization induced by progesterone in oral squamous cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729474/
https://www.ncbi.nlm.nih.gov/pubmed/26814504
http://dx.doi.org/10.1371/journal.pone.0147925
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