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Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice

Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the co...

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Autores principales: Nogueira, Denise Silva, Gazzinelli-Guimarães, Pedro Henrique, Barbosa, Fernando Sérgio, Resende, Nathália Maria, Silva, Caroline Cavalcanti, de Oliveira, Luciana Maria, Amorim, Chiara Cássia Oliveira, Oliveira, Fabrício Marcus Silva, Mattos, Matheus Silvério, Kraemer, Lucas Rocha, Caliari, Marcelo Vidigal, Gaze, Soraya, Bueno, Lilian Lacerda, Russo, Remo Castro, Fujiwara, Ricardo Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729520/
https://www.ncbi.nlm.nih.gov/pubmed/26814713
http://dx.doi.org/10.1371/journal.pntd.0004382
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author Nogueira, Denise Silva
Gazzinelli-Guimarães, Pedro Henrique
Barbosa, Fernando Sérgio
Resende, Nathália Maria
Silva, Caroline Cavalcanti
de Oliveira, Luciana Maria
Amorim, Chiara Cássia Oliveira
Oliveira, Fabrício Marcus Silva
Mattos, Matheus Silvério
Kraemer, Lucas Rocha
Caliari, Marcelo Vidigal
Gaze, Soraya
Bueno, Lilian Lacerda
Russo, Remo Castro
Fujiwara, Ricardo Toshio
author_facet Nogueira, Denise Silva
Gazzinelli-Guimarães, Pedro Henrique
Barbosa, Fernando Sérgio
Resende, Nathália Maria
Silva, Caroline Cavalcanti
de Oliveira, Luciana Maria
Amorim, Chiara Cássia Oliveira
Oliveira, Fabrício Marcus Silva
Mattos, Matheus Silvério
Kraemer, Lucas Rocha
Caliari, Marcelo Vidigal
Gaze, Soraya
Bueno, Lilian Lacerda
Russo, Remo Castro
Fujiwara, Ricardo Toshio
author_sort Nogueira, Denise Silva
collection PubMed
description Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the consequences to the host’s homeostasis are poorly understood. In this context, single and multiple exposures in mice were performed in order to characterize the parasitological, histopathological, tissue functional and immunological aspects of experimental larval ascariasis. The most important findings revealed that reinfected mice presented a significant reduction of parasite burden in the lung and an increase in the cellularity in the bronchoalveolar lavage (BAL) associated with a robust granulocytic pulmonary inflammation, leading to a severe impairment of respiratory function. Moreover, the multiple exposures to Ascaris elicited an increased number of circulating inflammatory cells as well as production of higher levels of systemic cytokines, mainly IL-4, IL-5, IL-6, IL-10, IL-17A and TNF-α when compared to single-infected animals. Taken together, our results suggest the intense pulmonary inflammation associated with a polarized systemic Th2/Th17 immune response are crucial to control larval migration after multiple exposures to Ascaris.
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spelling pubmed-47295202016-02-04 Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice Nogueira, Denise Silva Gazzinelli-Guimarães, Pedro Henrique Barbosa, Fernando Sérgio Resende, Nathália Maria Silva, Caroline Cavalcanti de Oliveira, Luciana Maria Amorim, Chiara Cássia Oliveira Oliveira, Fabrício Marcus Silva Mattos, Matheus Silvério Kraemer, Lucas Rocha Caliari, Marcelo Vidigal Gaze, Soraya Bueno, Lilian Lacerda Russo, Remo Castro Fujiwara, Ricardo Toshio PLoS Negl Trop Dis Research Article Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the consequences to the host’s homeostasis are poorly understood. In this context, single and multiple exposures in mice were performed in order to characterize the parasitological, histopathological, tissue functional and immunological aspects of experimental larval ascariasis. The most important findings revealed that reinfected mice presented a significant reduction of parasite burden in the lung and an increase in the cellularity in the bronchoalveolar lavage (BAL) associated with a robust granulocytic pulmonary inflammation, leading to a severe impairment of respiratory function. Moreover, the multiple exposures to Ascaris elicited an increased number of circulating inflammatory cells as well as production of higher levels of systemic cytokines, mainly IL-4, IL-5, IL-6, IL-10, IL-17A and TNF-α when compared to single-infected animals. Taken together, our results suggest the intense pulmonary inflammation associated with a polarized systemic Th2/Th17 immune response are crucial to control larval migration after multiple exposures to Ascaris. Public Library of Science 2016-01-27 /pmc/articles/PMC4729520/ /pubmed/26814713 http://dx.doi.org/10.1371/journal.pntd.0004382 Text en © 2016 Nogueira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nogueira, Denise Silva
Gazzinelli-Guimarães, Pedro Henrique
Barbosa, Fernando Sérgio
Resende, Nathália Maria
Silva, Caroline Cavalcanti
de Oliveira, Luciana Maria
Amorim, Chiara Cássia Oliveira
Oliveira, Fabrício Marcus Silva
Mattos, Matheus Silvério
Kraemer, Lucas Rocha
Caliari, Marcelo Vidigal
Gaze, Soraya
Bueno, Lilian Lacerda
Russo, Remo Castro
Fujiwara, Ricardo Toshio
Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice
title Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice
title_full Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice
title_fullStr Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice
title_full_unstemmed Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice
title_short Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice
title_sort multiple exposures to ascaris suum induce tissue injury and mixed th2/th17 immune response in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729520/
https://www.ncbi.nlm.nih.gov/pubmed/26814713
http://dx.doi.org/10.1371/journal.pntd.0004382
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