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Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability
Seeds enable plants to germinate and to grow in situations of limited availability of nutrients. The stable storage of different seed proteins is a remarkable presumption for successful germination and growth. These strategies have been adapted and used in several molecular farming projects. In this...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729946/ https://www.ncbi.nlm.nih.gov/pubmed/26858734 http://dx.doi.org/10.3389/fpls.2016.00006 |
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author | Weichert, Nicola Hauptmann, Valeska Helmold, Christine Conrad, Udo |
author_facet | Weichert, Nicola Hauptmann, Valeska Helmold, Christine Conrad, Udo |
author_sort | Weichert, Nicola |
collection | PubMed |
description | Seeds enable plants to germinate and to grow in situations of limited availability of nutrients. The stable storage of different seed proteins is a remarkable presumption for successful germination and growth. These strategies have been adapted and used in several molecular farming projects. In this study, we explore the benefits of seed-based expression to produce the high molecular weight spider silk protein FLAG using intein-based trans-splicing. Multimers larger than 460 kDa in size are routinely produced, which is above the native size of the FLAG protein. The storage of seeds for 8 weeks and 1 year at an ambient temperature of 15°C does not influence the accumulation level. Even the extended storage time does not influence the typical pattern of multimerized bands. These results show that seeds are the method of choice for stable accumulation of products of complex transgenes and have the capability for long-term storage at moderate conditions, an important feature for the development of suitable downstream processes. |
format | Online Article Text |
id | pubmed-4729946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47299462016-02-08 Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability Weichert, Nicola Hauptmann, Valeska Helmold, Christine Conrad, Udo Front Plant Sci Plant Science Seeds enable plants to germinate and to grow in situations of limited availability of nutrients. The stable storage of different seed proteins is a remarkable presumption for successful germination and growth. These strategies have been adapted and used in several molecular farming projects. In this study, we explore the benefits of seed-based expression to produce the high molecular weight spider silk protein FLAG using intein-based trans-splicing. Multimers larger than 460 kDa in size are routinely produced, which is above the native size of the FLAG protein. The storage of seeds for 8 weeks and 1 year at an ambient temperature of 15°C does not influence the accumulation level. Even the extended storage time does not influence the typical pattern of multimerized bands. These results show that seeds are the method of choice for stable accumulation of products of complex transgenes and have the capability for long-term storage at moderate conditions, an important feature for the development of suitable downstream processes. Frontiers Media S.A. 2016-01-28 /pmc/articles/PMC4729946/ /pubmed/26858734 http://dx.doi.org/10.3389/fpls.2016.00006 Text en Copyright © 2016 Weichert, Hauptmann, Helmold and Conrad. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Weichert, Nicola Hauptmann, Valeska Helmold, Christine Conrad, Udo Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability |
title | Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability |
title_full | Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability |
title_fullStr | Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability |
title_full_unstemmed | Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability |
title_short | Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability |
title_sort | seed-specific expression of spider silk protein multimers causes long-term stability |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729946/ https://www.ncbi.nlm.nih.gov/pubmed/26858734 http://dx.doi.org/10.3389/fpls.2016.00006 |
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