CRISPRi-Manipulation of Genetic Code Expansion via RF1 for Reassignment of Amber Codon in Bacteria

The precise engineering of proteins in bacteria via the amber codon has been hampered by the poor incorporation of unnatural amino acid (UAA). Here we explored the amber assignment as a sense codon for UAA by CRISPRi targeting release factor 1 (RF1). Scanning of RF1 gene with sgRNAs identified targe...

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Detalles Bibliográficos
Autores principales: Zhang, Bo, Yang, Qi, Chen, Jingxian, Wu, Ling, Yao, Tianzhuo, Wu, Yiming, Xu, Huan, Zhang, Lihe, Xia, Qing, Zhou, Demin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730227/
https://www.ncbi.nlm.nih.gov/pubmed/26818534
http://dx.doi.org/10.1038/srep20000

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