Cargando…
Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting
The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730313/ https://www.ncbi.nlm.nih.gov/pubmed/26751441 http://dx.doi.org/10.3390/ijms17010068 |
_version_ | 1782412373929230336 |
---|---|
author | Kim, Min Sup Park, Sang Jun Cho, Wheemoon Gu, Bon Kang Kim, Chun-Ho |
author_facet | Kim, Min Sup Park, Sang Jun Cho, Wheemoon Gu, Bon Kang Kim, Chun-Ho |
author_sort | Kim, Min Sup |
collection | PubMed |
description | The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 ± 3.27 to 75.29 ± 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model. |
format | Online Article Text |
id | pubmed-4730313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-47303132016-02-11 Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting Kim, Min Sup Park, Sang Jun Cho, Wheemoon Gu, Bon Kang Kim, Chun-Ho Int J Mol Sci Article The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 ± 3.27 to 75.29 ± 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model. MDPI 2016-01-07 /pmc/articles/PMC4730313/ /pubmed/26751441 http://dx.doi.org/10.3390/ijms17010068 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Min Sup Park, Sang Jun Cho, Wheemoon Gu, Bon Kang Kim, Chun-Ho Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting |
title | Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting |
title_full | Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting |
title_fullStr | Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting |
title_full_unstemmed | Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting |
title_short | Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting |
title_sort | neuropeptide substance-p-conjugated chitosan nanofibers as an active modulator of stem cell recruiting |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730313/ https://www.ncbi.nlm.nih.gov/pubmed/26751441 http://dx.doi.org/10.3390/ijms17010068 |
work_keys_str_mv | AT kimminsup neuropeptidesubstancepconjugatedchitosannanofibersasanactivemodulatorofstemcellrecruiting AT parksangjun neuropeptidesubstancepconjugatedchitosannanofibersasanactivemodulatorofstemcellrecruiting AT chowheemoon neuropeptidesubstancepconjugatedchitosannanofibersasanactivemodulatorofstemcellrecruiting AT gubonkang neuropeptidesubstancepconjugatedchitosannanofibersasanactivemodulatorofstemcellrecruiting AT kimchunho neuropeptidesubstancepconjugatedchitosannanofibersasanactivemodulatorofstemcellrecruiting |