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Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection

The d-galactose (d-gal)-injected animal model, which is typically established by administering consecutive subcutaneous d-gal injections to animals for approximately six or eight weeks, has been frequently used for aging research. In addition, this animal model has been demonstrated to accelerate ag...

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Autores principales: Liao, Chun-Hou, Chen, Bing-Huei, Chiang, Han-Sun, Chen, Chiu-Wei, Chen, Mei-Feng, Ke, Chih-Chun, Wang, Ya-Yun, Lin, Wei-Ning, Wang, Chi-Chung, Lin, Ying-Hung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730340/
https://www.ncbi.nlm.nih.gov/pubmed/26771610
http://dx.doi.org/10.3390/ijms17010098
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author Liao, Chun-Hou
Chen, Bing-Huei
Chiang, Han-Sun
Chen, Chiu-Wei
Chen, Mei-Feng
Ke, Chih-Chun
Wang, Ya-Yun
Lin, Wei-Ning
Wang, Chi-Chung
Lin, Ying-Hung
author_facet Liao, Chun-Hou
Chen, Bing-Huei
Chiang, Han-Sun
Chen, Chiu-Wei
Chen, Mei-Feng
Ke, Chih-Chun
Wang, Ya-Yun
Lin, Wei-Ning
Wang, Chi-Chung
Lin, Ying-Hung
author_sort Liao, Chun-Hou
collection PubMed
description The d-galactose (d-gal)-injected animal model, which is typically established by administering consecutive subcutaneous d-gal injections to animals for approximately six or eight weeks, has been frequently used for aging research. In addition, this animal model has been demonstrated to accelerate aging in the brain, kidneys, liver and blood cells. However, studies on aging in male reproductive organs that have used this animal model remain few. Therefore, the current study aimed to optimize a model of male reproductive aging by administering d-gal injections to male mice and to determine the possible mechanism expediting senescence processes during spermatogenesis. In this study, C57Bl/6 mice were randomized into five groups (each containing 8–10 mice according to the daily intraperitoneal injection of vehicle control or 100 or 200 mg/kg dosages of d-gal for a period of six or eight weeks). First, mice subjected to d-gal injections for six or eight weeks demonstrated considerably decreased superoxide dismutase activity in the serum and testis lysates compared to those in the control group. The lipid peroxidation in testis also increased in the d-gal-injected groups. Furthermore, the d-gal-injected groups exhibited a decreased ratio of testis weight/body weight and sperm count compared to the control group. The percentages of both immotile sperm and abnormal sperm increased considerably in the d-gal-injected groups compared to those of the control group. To determine the genes influenced by the d-gal injection during murine spermatogenesis, a c-DNA microarray was conducted to compare testicular RNA samples between the treated groups and the control group. The d-gal-injected groups exhibited RNA transcripts of nine spermatogenesis-related genes (Cycl2, Hk1, Pltp, Utp3, Cabyr, Zpbp2, Speer2, Csnka2ip and Katnb1) that were up- or down-regulated by at least two-fold compared to the control group. Several of these genes are critical for forming sperm-head morphologies or maintaining nuclear integration (e.g., cylicin, basic protein of sperm head cytoskeleton 2 (Cylc2), casein kinase 2, alpha prime interacting protein (Csnka2ip) and katanin p80 (WD40-containing) subunit B1 (Katnb1)). These results indicate that d-gal-injected mice are suitable for investigating male reproductive aging.
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spelling pubmed-47303402016-02-11 Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection Liao, Chun-Hou Chen, Bing-Huei Chiang, Han-Sun Chen, Chiu-Wei Chen, Mei-Feng Ke, Chih-Chun Wang, Ya-Yun Lin, Wei-Ning Wang, Chi-Chung Lin, Ying-Hung Int J Mol Sci Article The d-galactose (d-gal)-injected animal model, which is typically established by administering consecutive subcutaneous d-gal injections to animals for approximately six or eight weeks, has been frequently used for aging research. In addition, this animal model has been demonstrated to accelerate aging in the brain, kidneys, liver and blood cells. However, studies on aging in male reproductive organs that have used this animal model remain few. Therefore, the current study aimed to optimize a model of male reproductive aging by administering d-gal injections to male mice and to determine the possible mechanism expediting senescence processes during spermatogenesis. In this study, C57Bl/6 mice were randomized into five groups (each containing 8–10 mice according to the daily intraperitoneal injection of vehicle control or 100 or 200 mg/kg dosages of d-gal for a period of six or eight weeks). First, mice subjected to d-gal injections for six or eight weeks demonstrated considerably decreased superoxide dismutase activity in the serum and testis lysates compared to those in the control group. The lipid peroxidation in testis also increased in the d-gal-injected groups. Furthermore, the d-gal-injected groups exhibited a decreased ratio of testis weight/body weight and sperm count compared to the control group. The percentages of both immotile sperm and abnormal sperm increased considerably in the d-gal-injected groups compared to those of the control group. To determine the genes influenced by the d-gal injection during murine spermatogenesis, a c-DNA microarray was conducted to compare testicular RNA samples between the treated groups and the control group. The d-gal-injected groups exhibited RNA transcripts of nine spermatogenesis-related genes (Cycl2, Hk1, Pltp, Utp3, Cabyr, Zpbp2, Speer2, Csnka2ip and Katnb1) that were up- or down-regulated by at least two-fold compared to the control group. Several of these genes are critical for forming sperm-head morphologies or maintaining nuclear integration (e.g., cylicin, basic protein of sperm head cytoskeleton 2 (Cylc2), casein kinase 2, alpha prime interacting protein (Csnka2ip) and katanin p80 (WD40-containing) subunit B1 (Katnb1)). These results indicate that d-gal-injected mice are suitable for investigating male reproductive aging. MDPI 2016-01-13 /pmc/articles/PMC4730340/ /pubmed/26771610 http://dx.doi.org/10.3390/ijms17010098 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liao, Chun-Hou
Chen, Bing-Huei
Chiang, Han-Sun
Chen, Chiu-Wei
Chen, Mei-Feng
Ke, Chih-Chun
Wang, Ya-Yun
Lin, Wei-Ning
Wang, Chi-Chung
Lin, Ying-Hung
Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection
title Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection
title_full Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection
title_fullStr Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection
title_full_unstemmed Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection
title_short Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection
title_sort optimizing a male reproductive aging mouse model by d-galactose injection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730340/
https://www.ncbi.nlm.nih.gov/pubmed/26771610
http://dx.doi.org/10.3390/ijms17010098
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