Cargando…

Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney

Group V (GV) phospholipase A(2) (PLA(2)) is a member of the family of secreted PLA(2) (sPLA2) enzymes(.) This enzyme has been identified in several organs, including the kidney. However, the physiologic role of GV sPLA(2) in the maintenance of renal function remains unclear. We used mice lacking the...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva-Filho, João Luiz, Peruchetti, Diogo Barros, Moraes-Santos, Felipe, Landgraf, Sharon Schilling, Silva, Leandro Souza, Sirtoli, Gabriela Modenesi, Zamith-Miranda, Daniel, Takiya, Christina Maeda, Pinheiro, Ana Acacia Sá, Diaz, Bruno Lourenço, Caruso-Neves, Celso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731149/
https://www.ncbi.nlm.nih.gov/pubmed/26820468
http://dx.doi.org/10.1371/journal.pone.0147785
_version_ 1782412516899422208
author Silva-Filho, João Luiz
Peruchetti, Diogo Barros
Moraes-Santos, Felipe
Landgraf, Sharon Schilling
Silva, Leandro Souza
Sirtoli, Gabriela Modenesi
Zamith-Miranda, Daniel
Takiya, Christina Maeda
Pinheiro, Ana Acacia Sá
Diaz, Bruno Lourenço
Caruso-Neves, Celso
author_facet Silva-Filho, João Luiz
Peruchetti, Diogo Barros
Moraes-Santos, Felipe
Landgraf, Sharon Schilling
Silva, Leandro Souza
Sirtoli, Gabriela Modenesi
Zamith-Miranda, Daniel
Takiya, Christina Maeda
Pinheiro, Ana Acacia Sá
Diaz, Bruno Lourenço
Caruso-Neves, Celso
author_sort Silva-Filho, João Luiz
collection PubMed
description Group V (GV) phospholipase A(2) (PLA(2)) is a member of the family of secreted PLA(2) (sPLA2) enzymes(.) This enzyme has been identified in several organs, including the kidney. However, the physiologic role of GV sPLA(2) in the maintenance of renal function remains unclear. We used mice lacking the gene encoding GV sPLA(2) (Pla2g5(−/−)) and wild-type breeding pairs in the experiments. Mice were individually housed in metabolic cages and 48-h urine was collected for biochemical assays. Kidney samples were evaluated for glomerular morphology, renal fibrosis, and expression/activity of the (Na(+) + K(+))-ATPase α1 subunit. We observed that plasma creatinine levels were increased in Pla2g5(−/−) mice following by a decrease in creatinine clearance. The levels of urinary protein were higher in Pla2g5(−/−) mice than in the control group. Markers of tubular integrity and function such as γ-glutamyl transpeptidase, lactate dehydrogenase, and sodium excretion fraction (FE(Na)(+)) were also increased in Pla2g5(−/−) mice. The increased FE(Na)(+) observed in Pla2g5(−/−) mice was correlated to alterations in cortical (Na(+) + K(+)) ATPase activity/ expression. In addition, the kidney from Pla2g5(−/−) mice showed accumulation of matrix in corticomedullary glomeruli and tubulointerstitial fibrosis. These data suggest GV sPLA(2) is involved in the maintenance of tubular cell function and integrity, promoting sodium retention through increased cortical (Na(+) + K(+))-ATPase expression and activity.
format Online
Article
Text
id pubmed-4731149
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47311492016-02-04 Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney Silva-Filho, João Luiz Peruchetti, Diogo Barros Moraes-Santos, Felipe Landgraf, Sharon Schilling Silva, Leandro Souza Sirtoli, Gabriela Modenesi Zamith-Miranda, Daniel Takiya, Christina Maeda Pinheiro, Ana Acacia Sá Diaz, Bruno Lourenço Caruso-Neves, Celso PLoS One Research Article Group V (GV) phospholipase A(2) (PLA(2)) is a member of the family of secreted PLA(2) (sPLA2) enzymes(.) This enzyme has been identified in several organs, including the kidney. However, the physiologic role of GV sPLA(2) in the maintenance of renal function remains unclear. We used mice lacking the gene encoding GV sPLA(2) (Pla2g5(−/−)) and wild-type breeding pairs in the experiments. Mice were individually housed in metabolic cages and 48-h urine was collected for biochemical assays. Kidney samples were evaluated for glomerular morphology, renal fibrosis, and expression/activity of the (Na(+) + K(+))-ATPase α1 subunit. We observed that plasma creatinine levels were increased in Pla2g5(−/−) mice following by a decrease in creatinine clearance. The levels of urinary protein were higher in Pla2g5(−/−) mice than in the control group. Markers of tubular integrity and function such as γ-glutamyl transpeptidase, lactate dehydrogenase, and sodium excretion fraction (FE(Na)(+)) were also increased in Pla2g5(−/−) mice. The increased FE(Na)(+) observed in Pla2g5(−/−) mice was correlated to alterations in cortical (Na(+) + K(+)) ATPase activity/ expression. In addition, the kidney from Pla2g5(−/−) mice showed accumulation of matrix in corticomedullary glomeruli and tubulointerstitial fibrosis. These data suggest GV sPLA(2) is involved in the maintenance of tubular cell function and integrity, promoting sodium retention through increased cortical (Na(+) + K(+))-ATPase expression and activity. Public Library of Science 2016-01-28 /pmc/articles/PMC4731149/ /pubmed/26820468 http://dx.doi.org/10.1371/journal.pone.0147785 Text en © 2016 Silva-Filho et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Silva-Filho, João Luiz
Peruchetti, Diogo Barros
Moraes-Santos, Felipe
Landgraf, Sharon Schilling
Silva, Leandro Souza
Sirtoli, Gabriela Modenesi
Zamith-Miranda, Daniel
Takiya, Christina Maeda
Pinheiro, Ana Acacia Sá
Diaz, Bruno Lourenço
Caruso-Neves, Celso
Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney
title Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney
title_full Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney
title_fullStr Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney
title_full_unstemmed Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney
title_short Group V Secretory Phospholipase A(2) Is Involved in Tubular Integrity and Sodium Handling in the Kidney
title_sort group v secretory phospholipase a(2) is involved in tubular integrity and sodium handling in the kidney
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731149/
https://www.ncbi.nlm.nih.gov/pubmed/26820468
http://dx.doi.org/10.1371/journal.pone.0147785
work_keys_str_mv AT silvafilhojoaoluiz groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT peruchettidiogobarros groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT moraessantosfelipe groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT landgrafsharonschilling groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT silvaleandrosouza groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT sirtoligabrielamodenesi groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT zamithmirandadaniel groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT takiyachristinamaeda groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT pinheiroanaacaciasa groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT diazbrunolourenco groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney
AT carusonevescelso groupvsecretoryphospholipasea2isinvolvedintubularintegrityandsodiumhandlinginthekidney