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Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin

The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and...

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Autores principales: Dowell, Frederick, Cardman, Zena, Dasarathy, Srishti, Kellermann, Matthias Y., Lipp, Julius S., Ruff, S. Emil, Biddle, Jennifer F., McKay, Luke J., MacGregor, Barbara J., Lloyd, Karen G., Albert, Daniel B., Mendlovitz, Howard, Hinrichs, Kai-Uwe, Teske, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731509/
https://www.ncbi.nlm.nih.gov/pubmed/26858698
http://dx.doi.org/10.3389/fmicb.2016.00017
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author Dowell, Frederick
Cardman, Zena
Dasarathy, Srishti
Kellermann, Matthias Y.
Lipp, Julius S.
Ruff, S. Emil
Biddle, Jennifer F.
McKay, Luke J.
MacGregor, Barbara J.
Lloyd, Karen G.
Albert, Daniel B.
Mendlovitz, Howard
Hinrichs, Kai-Uwe
Teske, Andreas
author_facet Dowell, Frederick
Cardman, Zena
Dasarathy, Srishti
Kellermann, Matthias Y.
Lipp, Julius S.
Ruff, S. Emil
Biddle, Jennifer F.
McKay, Luke J.
MacGregor, Barbara J.
Lloyd, Karen G.
Albert, Daniel B.
Mendlovitz, Howard
Hinrichs, Kai-Uwe
Teske, Andreas
author_sort Dowell, Frederick
collection PubMed
description The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60°C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed “Mat Mound”) were characterized by porewater geochemistry of methane, C(2)–C(6) short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister clade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.
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spelling pubmed-47315092016-02-08 Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin Dowell, Frederick Cardman, Zena Dasarathy, Srishti Kellermann, Matthias Y. Lipp, Julius S. Ruff, S. Emil Biddle, Jennifer F. McKay, Luke J. MacGregor, Barbara J. Lloyd, Karen G. Albert, Daniel B. Mendlovitz, Howard Hinrichs, Kai-Uwe Teske, Andreas Front Microbiol Microbiology The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60°C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed “Mat Mound”) were characterized by porewater geochemistry of methane, C(2)–C(6) short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister clade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates. Frontiers Media S.A. 2016-01-29 /pmc/articles/PMC4731509/ /pubmed/26858698 http://dx.doi.org/10.3389/fmicb.2016.00017 Text en Copyright © 2016 Dowell, Cardman, Dasarathy, Kellermann, Lipp, Ruff, Biddle, McKay, MacGregor, Lloyd, Albert, Mendlovitz, Hinrichs and Teske. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dowell, Frederick
Cardman, Zena
Dasarathy, Srishti
Kellermann, Matthias Y.
Lipp, Julius S.
Ruff, S. Emil
Biddle, Jennifer F.
McKay, Luke J.
MacGregor, Barbara J.
Lloyd, Karen G.
Albert, Daniel B.
Mendlovitz, Howard
Hinrichs, Kai-Uwe
Teske, Andreas
Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin
title Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin
title_full Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin
title_fullStr Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin
title_full_unstemmed Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin
title_short Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin
title_sort microbial communities in methane- and short chain alkane-rich hydrothermal sediments of guaymas basin
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731509/
https://www.ncbi.nlm.nih.gov/pubmed/26858698
http://dx.doi.org/10.3389/fmicb.2016.00017
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