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Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures
Feline infectious peritonitis (FIP) results from mutations in the viral genome during a common feline enteric coronavirus (FECV) infection. Since many virological and immunological data on FECV infections are lacking, the present study investigated these missing links during experimental infection o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731813/ https://www.ncbi.nlm.nih.gov/pubmed/26822958 http://dx.doi.org/10.1038/srep20022 |
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author | Desmarets, Lowiese M. B. Vermeulen, Ben L. Theuns, Sebastiaan Conceição-Neto, Nádia Zeller, Mark Roukaerts, Inge D. M. Acar, Delphine D. Olyslaegers, Dominique A. J. Van Ranst, Marc Matthijnssens, Jelle Nauwynck, Hans J. |
author_facet | Desmarets, Lowiese M. B. Vermeulen, Ben L. Theuns, Sebastiaan Conceição-Neto, Nádia Zeller, Mark Roukaerts, Inge D. M. Acar, Delphine D. Olyslaegers, Dominique A. J. Van Ranst, Marc Matthijnssens, Jelle Nauwynck, Hans J. |
author_sort | Desmarets, Lowiese M. B. |
collection | PubMed |
description | Feline infectious peritonitis (FIP) results from mutations in the viral genome during a common feline enteric coronavirus (FECV) infection. Since many virological and immunological data on FECV infections are lacking, the present study investigated these missing links during experimental infection of three SPF cats with FECV strain UCD. Two cats showed mild clinical signs, faecal shedding of infectious virus from 4 dpi, a cell-associated viraemia at inconsistent time points from 5 dpi, a highly neutralising antibody response from 9 dpi, and no major abnormalities in leukocyte numbers. Faecal shedding lasted for 28–56 days, but virus shed during this stage was less infectious in enterocyte cultures and affected by mutations. Remarkably, in the other cat neither clinical signs nor acute shedding were seen, but virus was detected in blood cells from 3 dpi, and shedding of non-enterotropic, mutated viruses suddenly occurred from 14 dpi onwards. Neutralising antibodies arose from 21 dpi. Leukocyte numbers were not different compared to the other cats, except for the CD8(+) regulatory T cells. These data indicate that FECV can infect immune cells even in the absence of intestinal replication and raise the hypothesis that the gradual adaptation to these cells can allow non-enterotropic mutants to arise. |
format | Online Article Text |
id | pubmed-4731813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47318132016-02-04 Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures Desmarets, Lowiese M. B. Vermeulen, Ben L. Theuns, Sebastiaan Conceição-Neto, Nádia Zeller, Mark Roukaerts, Inge D. M. Acar, Delphine D. Olyslaegers, Dominique A. J. Van Ranst, Marc Matthijnssens, Jelle Nauwynck, Hans J. Sci Rep Article Feline infectious peritonitis (FIP) results from mutations in the viral genome during a common feline enteric coronavirus (FECV) infection. Since many virological and immunological data on FECV infections are lacking, the present study investigated these missing links during experimental infection of three SPF cats with FECV strain UCD. Two cats showed mild clinical signs, faecal shedding of infectious virus from 4 dpi, a cell-associated viraemia at inconsistent time points from 5 dpi, a highly neutralising antibody response from 9 dpi, and no major abnormalities in leukocyte numbers. Faecal shedding lasted for 28–56 days, but virus shed during this stage was less infectious in enterocyte cultures and affected by mutations. Remarkably, in the other cat neither clinical signs nor acute shedding were seen, but virus was detected in blood cells from 3 dpi, and shedding of non-enterotropic, mutated viruses suddenly occurred from 14 dpi onwards. Neutralising antibodies arose from 21 dpi. Leukocyte numbers were not different compared to the other cats, except for the CD8(+) regulatory T cells. These data indicate that FECV can infect immune cells even in the absence of intestinal replication and raise the hypothesis that the gradual adaptation to these cells can allow non-enterotropic mutants to arise. Nature Publishing Group 2016-01-29 /pmc/articles/PMC4731813/ /pubmed/26822958 http://dx.doi.org/10.1038/srep20022 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Desmarets, Lowiese M. B. Vermeulen, Ben L. Theuns, Sebastiaan Conceição-Neto, Nádia Zeller, Mark Roukaerts, Inge D. M. Acar, Delphine D. Olyslaegers, Dominique A. J. Van Ranst, Marc Matthijnssens, Jelle Nauwynck, Hans J. Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
title | Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
title_full | Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
title_fullStr | Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
title_full_unstemmed | Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
title_short | Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
title_sort | experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731813/ https://www.ncbi.nlm.nih.gov/pubmed/26822958 http://dx.doi.org/10.1038/srep20022 |
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