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Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein

[Image: see text] Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the an...

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Autores principales: Hanswillemenke, Alfred, Kuzdere, Tahsin, Vogel, Paul, Jékely, Gáspár, Stafforst, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731850/
https://www.ncbi.nlm.nih.gov/pubmed/26594902
http://dx.doi.org/10.1021/jacs.5b10216
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author Hanswillemenke, Alfred
Kuzdere, Tahsin
Vogel, Paul
Jékely, Gáspár
Stafforst, Thorsten
author_facet Hanswillemenke, Alfred
Kuzdere, Tahsin
Vogel, Paul
Jékely, Gáspár
Stafforst, Thorsten
author_sort Hanswillemenke, Alfred
collection PubMed
description [Image: see text] Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis dumerilii. A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by applying the SNAP-tag technology, a process that we demonstrate here to be readily triggered by light in vitro, in mammalian cell culture, and also in P. dumerilii. Lacking both sophisticated chemistry and extensive genetic engineering, this technology provides a convenient route for the light-dependent switching of protein isoforms. The presented strategy may also serve as a blue-print for the engineering of addressable machineries that apply tailored nucleic acid analogues to manipulate RNA or DNA site-specifically in living organisms.
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spelling pubmed-47318502016-02-10 Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein Hanswillemenke, Alfred Kuzdere, Tahsin Vogel, Paul Jékely, Gáspár Stafforst, Thorsten J Am Chem Soc [Image: see text] Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis dumerilii. A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by applying the SNAP-tag technology, a process that we demonstrate here to be readily triggered by light in vitro, in mammalian cell culture, and also in P. dumerilii. Lacking both sophisticated chemistry and extensive genetic engineering, this technology provides a convenient route for the light-dependent switching of protein isoforms. The presented strategy may also serve as a blue-print for the engineering of addressable machineries that apply tailored nucleic acid analogues to manipulate RNA or DNA site-specifically in living organisms. American Chemical Society 2015-11-23 2015-12-23 /pmc/articles/PMC4731850/ /pubmed/26594902 http://dx.doi.org/10.1021/jacs.5b10216 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Hanswillemenke, Alfred
Kuzdere, Tahsin
Vogel, Paul
Jékely, Gáspár
Stafforst, Thorsten
Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
title Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
title_full Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
title_fullStr Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
title_full_unstemmed Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
title_short Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
title_sort site-directed rna editing in vivo can be triggered by the light-driven assembly of an artificial riboprotein
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731850/
https://www.ncbi.nlm.nih.gov/pubmed/26594902
http://dx.doi.org/10.1021/jacs.5b10216
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