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Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein
[Image: see text] Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the an...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731850/ https://www.ncbi.nlm.nih.gov/pubmed/26594902 http://dx.doi.org/10.1021/jacs.5b10216 |
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author | Hanswillemenke, Alfred Kuzdere, Tahsin Vogel, Paul Jékely, Gáspár Stafforst, Thorsten |
author_facet | Hanswillemenke, Alfred Kuzdere, Tahsin Vogel, Paul Jékely, Gáspár Stafforst, Thorsten |
author_sort | Hanswillemenke, Alfred |
collection | PubMed |
description | [Image: see text] Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis dumerilii. A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by applying the SNAP-tag technology, a process that we demonstrate here to be readily triggered by light in vitro, in mammalian cell culture, and also in P. dumerilii. Lacking both sophisticated chemistry and extensive genetic engineering, this technology provides a convenient route for the light-dependent switching of protein isoforms. The presented strategy may also serve as a blue-print for the engineering of addressable machineries that apply tailored nucleic acid analogues to manipulate RNA or DNA site-specifically in living organisms. |
format | Online Article Text |
id | pubmed-4731850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-47318502016-02-10 Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein Hanswillemenke, Alfred Kuzdere, Tahsin Vogel, Paul Jékely, Gáspár Stafforst, Thorsten J Am Chem Soc [Image: see text] Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis dumerilii. A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by applying the SNAP-tag technology, a process that we demonstrate here to be readily triggered by light in vitro, in mammalian cell culture, and also in P. dumerilii. Lacking both sophisticated chemistry and extensive genetic engineering, this technology provides a convenient route for the light-dependent switching of protein isoforms. The presented strategy may also serve as a blue-print for the engineering of addressable machineries that apply tailored nucleic acid analogues to manipulate RNA or DNA site-specifically in living organisms. American Chemical Society 2015-11-23 2015-12-23 /pmc/articles/PMC4731850/ /pubmed/26594902 http://dx.doi.org/10.1021/jacs.5b10216 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Hanswillemenke, Alfred Kuzdere, Tahsin Vogel, Paul Jékely, Gáspár Stafforst, Thorsten Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein |
title | Site-Directed
RNA Editing in Vivo Can Be Triggered
by the Light-Driven Assembly of an Artificial Riboprotein |
title_full | Site-Directed
RNA Editing in Vivo Can Be Triggered
by the Light-Driven Assembly of an Artificial Riboprotein |
title_fullStr | Site-Directed
RNA Editing in Vivo Can Be Triggered
by the Light-Driven Assembly of an Artificial Riboprotein |
title_full_unstemmed | Site-Directed
RNA Editing in Vivo Can Be Triggered
by the Light-Driven Assembly of an Artificial Riboprotein |
title_short | Site-Directed
RNA Editing in Vivo Can Be Triggered
by the Light-Driven Assembly of an Artificial Riboprotein |
title_sort | site-directed
rna editing in vivo can be triggered
by the light-driven assembly of an artificial riboprotein |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731850/ https://www.ncbi.nlm.nih.gov/pubmed/26594902 http://dx.doi.org/10.1021/jacs.5b10216 |
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