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Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice

BACKGROUND: Bee venom acupuncture (BVA), a novel type of acupuncture therapy in which purified bee venom is injected into the specific acupuncture point on the diseased part of the body, is used primarily for relieving pain and other musculoskeletal symptoms. In the present study, therapeutic potent...

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Autores principales: Sur, Bongjun, Lee, Bombi, Yeom, Mijung, Hong, Ju-Hee, Kwon, Sunoh, Kim, Seung-Tae, Lee, Hyang Sook, Park, Hi-Joon, Lee, Hyejung, Hahm, Dae-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731956/
https://www.ncbi.nlm.nih.gov/pubmed/26825274
http://dx.doi.org/10.1186/s12906-016-1019-y
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author Sur, Bongjun
Lee, Bombi
Yeom, Mijung
Hong, Ju-Hee
Kwon, Sunoh
Kim, Seung-Tae
Lee, Hyang Sook
Park, Hi-Joon
Lee, Hyejung
Hahm, Dae-Hyun
author_facet Sur, Bongjun
Lee, Bombi
Yeom, Mijung
Hong, Ju-Hee
Kwon, Sunoh
Kim, Seung-Tae
Lee, Hyang Sook
Park, Hi-Joon
Lee, Hyejung
Hahm, Dae-Hyun
author_sort Sur, Bongjun
collection PubMed
description BACKGROUND: Bee venom acupuncture (BVA), a novel type of acupuncture therapy in which purified bee venom is injected into the specific acupuncture point on the diseased part of the body, is used primarily for relieving pain and other musculoskeletal symptoms. In the present study, therapeutic potential of BVA to improve atopic dermatitis, a representative allergic dysfunction, was evaluated in the mouse model of trimellitic anhydride (TMA)-induced skin impairment. METHODS: Mice were treated with 5 % TMA on the dorsal flank for sensitization and subsequently treated with 2 % TMA on the dorsum of both ears for an additional 12 days after a 3-day interval. From the 7(th) day of 2 % TMA treatment, bilateral subcutaneous injection of BV (BV, 0.3 mg/kg) was performed daily at BL40 acupuncture points (located behind the knee) 1 h before 2 % TMA treatment for 5 days. RESULTS: BVA treatment markedly inhibited the expression levels of both T helper cell type 1 (Th1) and Th2 cytokines in ear skin and lymph nodes of TMA-treated mice. Clinical features of AD-like symptoms such as ear skin symptom severity and thickness, inflammation, and lymph node weight were significantly alleviated by BV treatment. BV treatment also inhibited the proliferation and infiltration of T cells, the production of Th1 and Th2 cytokines, and the synthesis of interleukin (IL)-4 and immunoglobulin E (IgE)—typical allergic Th2 responses in blood. The inhibitory effect of BVA was more pronounced at BL40 acupoint than non-acupuncture point located at the base of the tail. CONCLUSIONS: These results indicate that BV injection at specific acupuncture points effectively alleviates AD-like skin lesions by inhibiting inflammatory and allergic responses in a TMA-induced contact hypersensitivity mouse model. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-016-1019-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-47319562016-01-30 Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice Sur, Bongjun Lee, Bombi Yeom, Mijung Hong, Ju-Hee Kwon, Sunoh Kim, Seung-Tae Lee, Hyang Sook Park, Hi-Joon Lee, Hyejung Hahm, Dae-Hyun BMC Complement Altern Med Research Article BACKGROUND: Bee venom acupuncture (BVA), a novel type of acupuncture therapy in which purified bee venom is injected into the specific acupuncture point on the diseased part of the body, is used primarily for relieving pain and other musculoskeletal symptoms. In the present study, therapeutic potential of BVA to improve atopic dermatitis, a representative allergic dysfunction, was evaluated in the mouse model of trimellitic anhydride (TMA)-induced skin impairment. METHODS: Mice were treated with 5 % TMA on the dorsal flank for sensitization and subsequently treated with 2 % TMA on the dorsum of both ears for an additional 12 days after a 3-day interval. From the 7(th) day of 2 % TMA treatment, bilateral subcutaneous injection of BV (BV, 0.3 mg/kg) was performed daily at BL40 acupuncture points (located behind the knee) 1 h before 2 % TMA treatment for 5 days. RESULTS: BVA treatment markedly inhibited the expression levels of both T helper cell type 1 (Th1) and Th2 cytokines in ear skin and lymph nodes of TMA-treated mice. Clinical features of AD-like symptoms such as ear skin symptom severity and thickness, inflammation, and lymph node weight were significantly alleviated by BV treatment. BV treatment also inhibited the proliferation and infiltration of T cells, the production of Th1 and Th2 cytokines, and the synthesis of interleukin (IL)-4 and immunoglobulin E (IgE)—typical allergic Th2 responses in blood. The inhibitory effect of BVA was more pronounced at BL40 acupoint than non-acupuncture point located at the base of the tail. CONCLUSIONS: These results indicate that BV injection at specific acupuncture points effectively alleviates AD-like skin lesions by inhibiting inflammatory and allergic responses in a TMA-induced contact hypersensitivity mouse model. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-016-1019-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-01-29 /pmc/articles/PMC4731956/ /pubmed/26825274 http://dx.doi.org/10.1186/s12906-016-1019-y Text en © Sur et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sur, Bongjun
Lee, Bombi
Yeom, Mijung
Hong, Ju-Hee
Kwon, Sunoh
Kim, Seung-Tae
Lee, Hyang Sook
Park, Hi-Joon
Lee, Hyejung
Hahm, Dae-Hyun
Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
title Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
title_full Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
title_fullStr Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
title_full_unstemmed Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
title_short Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
title_sort bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731956/
https://www.ncbi.nlm.nih.gov/pubmed/26825274
http://dx.doi.org/10.1186/s12906-016-1019-y
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