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Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor

Insulin is a key regulator in glucose homeostasis and its deficiency or alternations in the human body causes various types of diabetic disorders. In this paper, we present the development of a reduced graphene oxide (rGO) modified interdigitated chain electrode (ICE) for direct capacitive detection...

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Detalles Bibliográficos
Autores principales: Yagati, Ajay Kumar, Park, Jinsoo, Cho, Sungbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732142/
https://www.ncbi.nlm.nih.gov/pubmed/26784202
http://dx.doi.org/10.3390/s16010109
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author Yagati, Ajay Kumar
Park, Jinsoo
Cho, Sungbo
author_facet Yagati, Ajay Kumar
Park, Jinsoo
Cho, Sungbo
author_sort Yagati, Ajay Kumar
collection PubMed
description Insulin is a key regulator in glucose homeostasis and its deficiency or alternations in the human body causes various types of diabetic disorders. In this paper, we present the development of a reduced graphene oxide (rGO) modified interdigitated chain electrode (ICE) for direct capacitive detection of insulin. The impedance properties of rGO-ICE were characterized by equivalent circuit modeling. After an electrochemical deposition of rGO on ICE, the electrode was modified with self-assembled monolayers and insulin antibodies in order to achieve insulin binding reactions. The impedance spectra and capacitances were measured with respect to the concentrations of insulin and the capacitance change (ΔC) was analyzed to quantify insulin concentration. The antibody immobilized electrode showed an increment of ΔC according to the insulin concentration in human serum ranging from 1 ng/mL to 10 µg/mL. The proposed sensor is feasible for label-free and real-time measuring of the biomarker and for point-of-care diagnosis.
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spelling pubmed-47321422016-02-12 Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor Yagati, Ajay Kumar Park, Jinsoo Cho, Sungbo Sensors (Basel) Article Insulin is a key regulator in glucose homeostasis and its deficiency or alternations in the human body causes various types of diabetic disorders. In this paper, we present the development of a reduced graphene oxide (rGO) modified interdigitated chain electrode (ICE) for direct capacitive detection of insulin. The impedance properties of rGO-ICE were characterized by equivalent circuit modeling. After an electrochemical deposition of rGO on ICE, the electrode was modified with self-assembled monolayers and insulin antibodies in order to achieve insulin binding reactions. The impedance spectra and capacitances were measured with respect to the concentrations of insulin and the capacitance change (ΔC) was analyzed to quantify insulin concentration. The antibody immobilized electrode showed an increment of ΔC according to the insulin concentration in human serum ranging from 1 ng/mL to 10 µg/mL. The proposed sensor is feasible for label-free and real-time measuring of the biomarker and for point-of-care diagnosis. MDPI 2016-01-15 /pmc/articles/PMC4732142/ /pubmed/26784202 http://dx.doi.org/10.3390/s16010109 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yagati, Ajay Kumar
Park, Jinsoo
Cho, Sungbo
Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor
title Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor
title_full Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor
title_fullStr Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor
title_full_unstemmed Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor
title_short Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor
title_sort reduced graphene oxide modified the interdigitated chain electrode for an insulin sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732142/
https://www.ncbi.nlm.nih.gov/pubmed/26784202
http://dx.doi.org/10.3390/s16010109
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