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Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal
Activation of leukemia inhibitor factor (LIF)–Stat3 or Wnt/β-catenin signaling promotes mouse embryonic stem cell (mESC) self-renewal. A myriad of downstream targets have been identified in the individual signal pathways, but their common targets remain largely elusive. In this study, we found that...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732286/ https://www.ncbi.nlm.nih.gov/pubmed/26598557 http://dx.doi.org/10.1242/jcs.177675 |
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author | Ye, Shoudong Zhang, Dongming Cheng, Fei Wilson, Daniel Mackay, Jeffrey He, Kan Ban, Qian Lv, Feng Huang, Saifei Liu, Dahai Ying, Qi-Long |
author_facet | Ye, Shoudong Zhang, Dongming Cheng, Fei Wilson, Daniel Mackay, Jeffrey He, Kan Ban, Qian Lv, Feng Huang, Saifei Liu, Dahai Ying, Qi-Long |
author_sort | Ye, Shoudong |
collection | PubMed |
description | Activation of leukemia inhibitor factor (LIF)–Stat3 or Wnt/β-catenin signaling promotes mouse embryonic stem cell (mESC) self-renewal. A myriad of downstream targets have been identified in the individual signal pathways, but their common targets remain largely elusive. In this study, we found that the LIF–Stat3 and Wnt/β-catenin signaling pathways converge on Sp5 to promote mESC self-renewal. Forced Sp5 expression can reproduce partial effects of Wnt/β-catenin signaling but mimics most features of LIF–Stat3 signaling to maintain undifferentiated mESCs. Moreover, Sp5 is able to convert mouse epiblast stem cells into a naïve pluripotent state. Thus, Sp5 is an important component of the regulatory network governing mESC naïve pluripotency. |
format | Online Article Text |
id | pubmed-4732286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47322862016-02-09 Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal Ye, Shoudong Zhang, Dongming Cheng, Fei Wilson, Daniel Mackay, Jeffrey He, Kan Ban, Qian Lv, Feng Huang, Saifei Liu, Dahai Ying, Qi-Long J Cell Sci Short Report Activation of leukemia inhibitor factor (LIF)–Stat3 or Wnt/β-catenin signaling promotes mouse embryonic stem cell (mESC) self-renewal. A myriad of downstream targets have been identified in the individual signal pathways, but their common targets remain largely elusive. In this study, we found that the LIF–Stat3 and Wnt/β-catenin signaling pathways converge on Sp5 to promote mESC self-renewal. Forced Sp5 expression can reproduce partial effects of Wnt/β-catenin signaling but mimics most features of LIF–Stat3 signaling to maintain undifferentiated mESCs. Moreover, Sp5 is able to convert mouse epiblast stem cells into a naïve pluripotent state. Thus, Sp5 is an important component of the regulatory network governing mESC naïve pluripotency. The Company of Biologists Ltd 2016-01-15 /pmc/articles/PMC4732286/ /pubmed/26598557 http://dx.doi.org/10.1242/jcs.177675 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Short Report Ye, Shoudong Zhang, Dongming Cheng, Fei Wilson, Daniel Mackay, Jeffrey He, Kan Ban, Qian Lv, Feng Huang, Saifei Liu, Dahai Ying, Qi-Long Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal |
title | Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal |
title_full | Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal |
title_fullStr | Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal |
title_full_unstemmed | Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal |
title_short | Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal |
title_sort | wnt/β-catenin and lif–stat3 signaling pathways converge on sp5 to promote mouse embryonic stem cell self-renewal |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732286/ https://www.ncbi.nlm.nih.gov/pubmed/26598557 http://dx.doi.org/10.1242/jcs.177675 |
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