Cargando…
Validation of the i-STAT system for the analysis of blood parameters in fish
Portable clinical analysers, such as the i-STAT system, are increasingly being used for blood analysis in animal ecology and physiology because of their portability and easy operation. Although originally conceived for clinical application and to replace robust but lengthy techniques, researchers ha...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732487/ https://www.ncbi.nlm.nih.gov/pubmed/27293658 http://dx.doi.org/10.1093/conphys/cou037 |
_version_ | 1782412714661904384 |
---|---|
author | Harter, T. S. Shartau, R. B. Brauner, C. J. Farrell, A. P. |
author_facet | Harter, T. S. Shartau, R. B. Brauner, C. J. Farrell, A. P. |
author_sort | Harter, T. S. |
collection | PubMed |
description | Portable clinical analysers, such as the i-STAT system, are increasingly being used for blood analysis in animal ecology and physiology because of their portability and easy operation. Although originally conceived for clinical application and to replace robust but lengthy techniques, researchers have extended the use of the i-STAT system outside of humans and even to poikilothermic fish, with only limited validation. The present study analysed a range of blood parameters [pH, haematocrit (Hct), haemoglobin (Hb), HCO(3)(−), partial pressure of CO(2) (PCO(2)), partial pressure of O(2) (PO(2)), Hb saturation (sO(2)) and Na(+) concentration] in a model teleost fish (rainbow trout, Oncorhynchus mykiss) using the i-STAT system (CG8+ cartridges) and established laboratory techniques. This methodological comparison was performed at two temperatures (10 and 20°C), two haematocrits (low and high) and three PCO(2) levels (0.5, 1.0 and 1.5%). Our results indicate that pH was measured accurately with the i-STAT system over a physiological pH range and using the i-STAT temperature correction. Haematocrit was consistently underestimated by the i-STAT, while the measurements of Na(+), PCO(2), HCO(3)(−) and PO(2) were variably inaccurate over the range of values typically found in fish. The algorithm that the i-STAT uses to calculate sO(2) did not yield meaningful results on rainbow trout blood. Application of conversion factors to correct i-STAT measurements is not recommended, due to significant effects of temperature, Hct and PCO(2) on the measurement errors and complex interactions may exist. In conclusion, the i-STAT system can easily generate fast results from rainbow trout whole blood, but many are inaccurate values. |
format | Online Article Text |
id | pubmed-4732487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47324872016-06-10 Validation of the i-STAT system for the analysis of blood parameters in fish Harter, T. S. Shartau, R. B. Brauner, C. J. Farrell, A. P. Conserv Physiol Research Articles Portable clinical analysers, such as the i-STAT system, are increasingly being used for blood analysis in animal ecology and physiology because of their portability and easy operation. Although originally conceived for clinical application and to replace robust but lengthy techniques, researchers have extended the use of the i-STAT system outside of humans and even to poikilothermic fish, with only limited validation. The present study analysed a range of blood parameters [pH, haematocrit (Hct), haemoglobin (Hb), HCO(3)(−), partial pressure of CO(2) (PCO(2)), partial pressure of O(2) (PO(2)), Hb saturation (sO(2)) and Na(+) concentration] in a model teleost fish (rainbow trout, Oncorhynchus mykiss) using the i-STAT system (CG8+ cartridges) and established laboratory techniques. This methodological comparison was performed at two temperatures (10 and 20°C), two haematocrits (low and high) and three PCO(2) levels (0.5, 1.0 and 1.5%). Our results indicate that pH was measured accurately with the i-STAT system over a physiological pH range and using the i-STAT temperature correction. Haematocrit was consistently underestimated by the i-STAT, while the measurements of Na(+), PCO(2), HCO(3)(−) and PO(2) were variably inaccurate over the range of values typically found in fish. The algorithm that the i-STAT uses to calculate sO(2) did not yield meaningful results on rainbow trout blood. Application of conversion factors to correct i-STAT measurements is not recommended, due to significant effects of temperature, Hct and PCO(2) on the measurement errors and complex interactions may exist. In conclusion, the i-STAT system can easily generate fast results from rainbow trout whole blood, but many are inaccurate values. Oxford University Press 2014-09-03 /pmc/articles/PMC4732487/ /pubmed/27293658 http://dx.doi.org/10.1093/conphys/cou037 Text en © The Author 2014. Published by Oxford University Press and the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Harter, T. S. Shartau, R. B. Brauner, C. J. Farrell, A. P. Validation of the i-STAT system for the analysis of blood parameters in fish |
title | Validation of the i-STAT system for the analysis of blood parameters in fish |
title_full | Validation of the i-STAT system for the analysis of blood parameters in fish |
title_fullStr | Validation of the i-STAT system for the analysis of blood parameters in fish |
title_full_unstemmed | Validation of the i-STAT system for the analysis of blood parameters in fish |
title_short | Validation of the i-STAT system for the analysis of blood parameters in fish |
title_sort | validation of the i-stat system for the analysis of blood parameters in fish |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732487/ https://www.ncbi.nlm.nih.gov/pubmed/27293658 http://dx.doi.org/10.1093/conphys/cou037 |
work_keys_str_mv | AT harterts validationoftheistatsystemfortheanalysisofbloodparametersinfish AT shartaurb validationoftheistatsystemfortheanalysisofbloodparametersinfish AT braunercj validationoftheistatsystemfortheanalysisofbloodparametersinfish AT farrellap validationoftheistatsystemfortheanalysisofbloodparametersinfish |