Cargando…

Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions

To gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (NAFLD), some intestinal and lactic acid bacteria and one yeast (Anaerostipes caccae, Bacteroides thetaiotaomicron, Bifidobacterium longum, Enterococcus fecalis, Escherichia coli, Lactobacillu...

Descripción completa

Detalles Bibliográficos
Autores principales: Elshaghabee, Fouad M. F., Bockelmann, Wilhelm, Meske, Diana, de Vrese, Michael, Walte, Hans-Georg, Schrezenmeir, Juergen, Heller, Knut J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732544/
https://www.ncbi.nlm.nih.gov/pubmed/26858714
http://dx.doi.org/10.3389/fmicb.2016.00047
_version_ 1782412724187168768
author Elshaghabee, Fouad M. F.
Bockelmann, Wilhelm
Meske, Diana
de Vrese, Michael
Walte, Hans-Georg
Schrezenmeir, Juergen
Heller, Knut J.
author_facet Elshaghabee, Fouad M. F.
Bockelmann, Wilhelm
Meske, Diana
de Vrese, Michael
Walte, Hans-Georg
Schrezenmeir, Juergen
Heller, Knut J.
author_sort Elshaghabee, Fouad M. F.
collection PubMed
description To gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (NAFLD), some intestinal and lactic acid bacteria and one yeast (Anaerostipes caccae, Bacteroides thetaiotaomicron, Bifidobacterium longum, Enterococcus fecalis, Escherichia coli, Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus plantarum, Weissella confusa, Saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol when grown on different carbohydrates: hexoses (glucose and fructose), pentoses (arabinose and ribose), disaccharides (lactose and lactulose), and inulin. Highest amounts of ethanol were produced by S. cerevisiae, L. fermentum, and W. confusa on glucose and by S. cerevisiae and W. confusa on fructose. Due to mannitol-dehydrogenase expressed in L. fermentum, ethanol production on fructose was significantly (P < 0.05) reduced. Pyruvate and citrate, two potential electron acceptors for regeneration of NAD(+)/NADP(+), drastically reduced ethanol production with acetate produced instead in L. fermentum grown on glucose and W. confusa grown on glucose and fructose, respectively. In fecal slurries prepared from feces of four overweight volunteers, ethanol was found to be produced upon addition of fructose. Addition of A. caccae, L. acidophilus, L. fermentum, as well as citrate and pyruvate, respectively, abolished ethanol production. However, addition of W. confusa resulted in significantly (P < 0.05) increased production of ethanol. These results indicate that microorganisms like W. confusa, a hetero-fermentative, mannitol-dehydrogenase negative lactic acid bacterium, may promote NAFLD through ethanol produced from sugar fermentation, while other intestinal bacteria and homo- and hetero-fermentative but mannitol-dehydrogenase positive lactic acid bacteria may not promote NAFLD. Also, our studies indicate that dietary factors interfering with gastrointestinal microbiota and microbial metabolism may be important in preventing or promoting NAFLD.
format Online
Article
Text
id pubmed-4732544
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-47325442016-02-08 Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions Elshaghabee, Fouad M. F. Bockelmann, Wilhelm Meske, Diana de Vrese, Michael Walte, Hans-Georg Schrezenmeir, Juergen Heller, Knut J. Front Microbiol Microbiology To gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (NAFLD), some intestinal and lactic acid bacteria and one yeast (Anaerostipes caccae, Bacteroides thetaiotaomicron, Bifidobacterium longum, Enterococcus fecalis, Escherichia coli, Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus plantarum, Weissella confusa, Saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol when grown on different carbohydrates: hexoses (glucose and fructose), pentoses (arabinose and ribose), disaccharides (lactose and lactulose), and inulin. Highest amounts of ethanol were produced by S. cerevisiae, L. fermentum, and W. confusa on glucose and by S. cerevisiae and W. confusa on fructose. Due to mannitol-dehydrogenase expressed in L. fermentum, ethanol production on fructose was significantly (P < 0.05) reduced. Pyruvate and citrate, two potential electron acceptors for regeneration of NAD(+)/NADP(+), drastically reduced ethanol production with acetate produced instead in L. fermentum grown on glucose and W. confusa grown on glucose and fructose, respectively. In fecal slurries prepared from feces of four overweight volunteers, ethanol was found to be produced upon addition of fructose. Addition of A. caccae, L. acidophilus, L. fermentum, as well as citrate and pyruvate, respectively, abolished ethanol production. However, addition of W. confusa resulted in significantly (P < 0.05) increased production of ethanol. These results indicate that microorganisms like W. confusa, a hetero-fermentative, mannitol-dehydrogenase negative lactic acid bacterium, may promote NAFLD through ethanol produced from sugar fermentation, while other intestinal bacteria and homo- and hetero-fermentative but mannitol-dehydrogenase positive lactic acid bacteria may not promote NAFLD. Also, our studies indicate that dietary factors interfering with gastrointestinal microbiota and microbial metabolism may be important in preventing or promoting NAFLD. Frontiers Media S.A. 2016-01-29 /pmc/articles/PMC4732544/ /pubmed/26858714 http://dx.doi.org/10.3389/fmicb.2016.00047 Text en Copyright © 2016 Elshaghabee, Bockelmann, Meske, de Vrese, Walte, Schrezenmeir and Heller. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Elshaghabee, Fouad M. F.
Bockelmann, Wilhelm
Meske, Diana
de Vrese, Michael
Walte, Hans-Georg
Schrezenmeir, Juergen
Heller, Knut J.
Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions
title Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions
title_full Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions
title_fullStr Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions
title_full_unstemmed Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions
title_short Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions
title_sort ethanol production by selected intestinal microorganisms and lactic acid bacteria growing under different nutritional conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732544/
https://www.ncbi.nlm.nih.gov/pubmed/26858714
http://dx.doi.org/10.3389/fmicb.2016.00047
work_keys_str_mv AT elshaghabeefouadmf ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions
AT bockelmannwilhelm ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions
AT meskediana ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions
AT devresemichael ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions
AT waltehansgeorg ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions
AT schrezenmeirjuergen ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions
AT hellerknutj ethanolproductionbyselectedintestinalmicroorganismsandlacticacidbacteriagrowingunderdifferentnutritionalconditions