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RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA), an Assay for Detecting RNA-Protein Complexes in Intact Cells

Techniques for studying RNA-protein interactions have lagged behind those for DNA-protein complexes as a consequence of the complexities associated with working with RNA. Here we present a method for the modification of the existing In Situ Hybridisation–Proximity Ligation Assay (ISH-PLA) protocol t...

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Detalles Bibliográficos
Autores principales: Roussis, Ioannis M., Guille, Matthew, Myers, Fiona A., Scarlett, Garry P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732756/
https://www.ncbi.nlm.nih.gov/pubmed/26824753
http://dx.doi.org/10.1371/journal.pone.0147967

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