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Histatin-1 Expression in Human Lacrimal Epithelium
BACKGROUND: Study of human lacrimal cell biology is limited by poor access to tissue samples, heterogeneous cell composition of tissue and a lack of established lacrimal epithelial markers. In order to further our understanding of lacrimal cell biology, we sought to find a better marker for human la...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732786/ https://www.ncbi.nlm.nih.gov/pubmed/26824896 http://dx.doi.org/10.1371/journal.pone.0148018 |
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author | Shah, Dhara Ali, Marwan Pasha, Zeeshan Jaboori, Assraa Jassim Jassim, Sarmad H. Jain, Sandeep Aakalu, Vinay K. |
author_facet | Shah, Dhara Ali, Marwan Pasha, Zeeshan Jaboori, Assraa Jassim Jassim, Sarmad H. Jain, Sandeep Aakalu, Vinay K. |
author_sort | Shah, Dhara |
collection | PubMed |
description | BACKGROUND: Study of human lacrimal cell biology is limited by poor access to tissue samples, heterogeneous cell composition of tissue and a lack of established lacrimal epithelial markers. In order to further our understanding of lacrimal cell biology, we sought to find a better marker for human lacrimal epithelial cells, compared to what has been reported in the literature. METHODS: We utilized human Muller’s muscle conjunctival resection (MMCR) specimens containing accessory lacrimal gland (ALG) and cadaveric main lacrimal gland (MLG) as sources of lacrimal tissue. Candidate markers were sought using human ALG tissue from MMCR specimens, isolated by laser capture microdissection (LCM). Affymetrix® analysis was performed on total RNA isolated from FFPE samples to profile transcription in ALG. MMCR tissue sections were assessed by immunofluorescence using antibodies for histatin-1, lactoferrin, E-cadherin (E-cad) and alpha-smooth muscle actin (ASMA). Reverse transcriptase polymerase chain reaction (RT-PCR) analysis was performed to analyze the expression of histatin-1, E-cad and lactoferrin from cadaveric MLG. RESULTS: Histatin-1 is expressed in ALG and MLG, localizes to lacrimal epithelium, and to a greater degree than do other putative lacrimal epithelial markers. CONCLUSIONS: Histatin-1 is a good marker for human lacrimal epithelium in ALG and MLG and can be used to identify lacrimal cells in future studies. |
format | Online Article Text |
id | pubmed-4732786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47327862016-02-04 Histatin-1 Expression in Human Lacrimal Epithelium Shah, Dhara Ali, Marwan Pasha, Zeeshan Jaboori, Assraa Jassim Jassim, Sarmad H. Jain, Sandeep Aakalu, Vinay K. PLoS One Research Article BACKGROUND: Study of human lacrimal cell biology is limited by poor access to tissue samples, heterogeneous cell composition of tissue and a lack of established lacrimal epithelial markers. In order to further our understanding of lacrimal cell biology, we sought to find a better marker for human lacrimal epithelial cells, compared to what has been reported in the literature. METHODS: We utilized human Muller’s muscle conjunctival resection (MMCR) specimens containing accessory lacrimal gland (ALG) and cadaveric main lacrimal gland (MLG) as sources of lacrimal tissue. Candidate markers were sought using human ALG tissue from MMCR specimens, isolated by laser capture microdissection (LCM). Affymetrix® analysis was performed on total RNA isolated from FFPE samples to profile transcription in ALG. MMCR tissue sections were assessed by immunofluorescence using antibodies for histatin-1, lactoferrin, E-cadherin (E-cad) and alpha-smooth muscle actin (ASMA). Reverse transcriptase polymerase chain reaction (RT-PCR) analysis was performed to analyze the expression of histatin-1, E-cad and lactoferrin from cadaveric MLG. RESULTS: Histatin-1 is expressed in ALG and MLG, localizes to lacrimal epithelium, and to a greater degree than do other putative lacrimal epithelial markers. CONCLUSIONS: Histatin-1 is a good marker for human lacrimal epithelium in ALG and MLG and can be used to identify lacrimal cells in future studies. Public Library of Science 2016-01-29 /pmc/articles/PMC4732786/ /pubmed/26824896 http://dx.doi.org/10.1371/journal.pone.0148018 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Shah, Dhara Ali, Marwan Pasha, Zeeshan Jaboori, Assraa Jassim Jassim, Sarmad H. Jain, Sandeep Aakalu, Vinay K. Histatin-1 Expression in Human Lacrimal Epithelium |
title | Histatin-1 Expression in Human Lacrimal Epithelium |
title_full | Histatin-1 Expression in Human Lacrimal Epithelium |
title_fullStr | Histatin-1 Expression in Human Lacrimal Epithelium |
title_full_unstemmed | Histatin-1 Expression in Human Lacrimal Epithelium |
title_short | Histatin-1 Expression in Human Lacrimal Epithelium |
title_sort | histatin-1 expression in human lacrimal epithelium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732786/ https://www.ncbi.nlm.nih.gov/pubmed/26824896 http://dx.doi.org/10.1371/journal.pone.0148018 |
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