Cargando…

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples

Digital PCR (dPCR) is being increasingly used for the quantification of sequence variations, including single nucleotide polymorphisms (SNPs), due to its high accuracy and precision in comparison with techniques such as quantitative PCR (qPCR) and melt curve analysis. To develop and evaluate dPCR fo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Whale, Alexandra S., Bushell, Claire A., Grant, Paul R., Cowen, Simon, Gutierrez-Aguirre, Ion, O'Sullivan, Denise M., Žel, Jana, Milavec, Mojca, Foy, Carole A., Nastouli, Eleni, Garson, Jeremy A., Huggett, Jim F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2016
Materias:	Virology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4733194/ https://www.ncbi.nlm.nih.gov/pubmed/26659206 http://dx.doi.org/10.1128/JCM.02611-15

Ejemplares similares

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR
por: Busby, Eloise, et al.
Publicado: (2017)

Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples
por: Whale, Alexandra S., et al.
Publicado: (2013)

Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
por: Pavšič, Jernej, et al.
Publicado: (2015)

Digital PCR for direct quantification of viruses without DNA extraction
por: Pavšič, Jernej, et al.
Publicado: (2015)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Applicability of Control Materials To Support Gene Promoter Characterization and Expression in Engineered Cells Using Digital PCR
por: Fernandez-Gonzalez, Ana, et al.
Publicado: (2022)

Digital PCR dynamic range is approaching that of real-time quantitative PCR
por: Jones, Gerwyn M., et al.
Publicado: (2016)

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA
por: Pavšič, Jernej, et al.
Publicado: (2017)

Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR
por: Morisset, Dany, et al.
Publicado: (2013)

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification
por: Devonshire, Alison S., et al.
Publicado: (2014)

Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology
por: Belák, S., et al.
Publicado: (1993)

Utility of Stool PCR for the Diagnosis of COVID-19: Comparison of Two Commercial Platforms
por: Szymczak, Wendy A., et al.
Publicado: (2020)

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
por: LIU, Dafei, et al.
Publicado: (2018)

Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing
por: Zowawi, Hosam M., et al.
Publicado: (2021)

Development and Evaluation of an Enterovirus D68 Real-Time Reverse Transcriptase PCR Assay
por: Wylie, Todd N., et al.
Publicado: (2015)

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19
por: Nagura-Ikeda, Mayu, et al.
Publicado: (2020)

Fast and Accurate Multiplex Identification and Quantification of Seven Genetically Modified Soybean Lines Using Six-Color Digital PCR
por: Bogožalec Košir, Alexandra, et al.
Publicado: (2023)

Quantitative PCR detection of feline morbillivirus in cat urine samples
por: FURUYA, Tetsuya, et al.
Publicado: (2015)

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis
por: Devonshire, Alison S., et al.
Publicado: (2016)

Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay
por: Potter, Robert F., et al.
Publicado: (2021)

Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR
por: Rogers, Amy A., et al.
Publicado: (2020)

Clinical Performance and Trends during the First Two Months of Monkeypox Virus PCR Testing at Two United States Reference Labs
por: Lieberman, Nicole A. P., et al.
Publicado: (2022)

Prospective Performance Evaluation of the miDiagnostics COVID-19 PCR Test for Rapid SARS-CoV-2 Detection on Nasopharyngeal Swabs
por: Bos, David A. G., et al.
Publicado: (2023)

QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES
por: STAGGEMEIER, Rodrigo, et al.
Publicado: (2015)

Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification
por: Yeung, Priscilla S.-W., et al.
Publicado: (2022)

Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America()()
por: Edwards, Thomas, et al.
Publicado: (2017)

Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2
por: Craney, Arryn R., et al.
Publicado: (2020)

Development of a novel detection system for microbes from bovine diarrhea by real-time PCR
por: TSUCHIAKA, Shinobu, et al.
Publicado: (2015)

Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
por: PIEWBANG, Chutchai, et al.
Publicado: (2016)

Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus
por: NISHIKAKU, Kohei, et al.
Publicado: (2019)

Impact of SARS-CoV-2 Variants on the Analytical Sensitivity of rRT-PCR Assays
por: Chen, Yuqing, et al.
Publicado: (2022)

Factors associated with weak positive SARS-CoV-2 diagnosis by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR)
por: Rawat, Priyank, et al.
Publicado: (2022)

Fundamentals of multiplexing with digital PCR
por: Whale, Alexandra S., et al.
Publicado: (2016)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Development of a PCR assay for detection and differentiation of Muscovy duck and goose parvoviruses based on NS gene characterization
por: WAN, Chunhe, et al.
Publicado: (2018)

Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses
por: WAN, Chun-He, et al.
Publicado: (2016)

Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases
por: SUNAGA, Fujiko, et al.
Publicado: (2019)

Highly Sensitive Lineage Discrimination of SARS-CoV-2 Variants through Allele-Specific Probe PCR
por: Ratcliff, Jeremy, et al.
Publicado: (2022)

Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
por: TAKANO, Tomomi, et al.
Publicado: (2021)

Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex
por: KISHIMOTO, Mai, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_15jlebn34vnet8op4neoodn6mn