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Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats
The aim of the present study was to investigate the inhibitory effects of (90)Sr-(90)Y β-irradiation in a rat model of alkali burn-induced corneal neovascularization (CNV). Alkali burn-induced CNV was induced in the right eyes of 30 female Wistar rats, which were randomly divided into the following...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734155/ https://www.ncbi.nlm.nih.gov/pubmed/26893623 http://dx.doi.org/10.3892/etm.2015.2907 |
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author | LIN, YUANQIANG MA, QINGJIE LIN, SHAN ZHOU, HONGYAN WEN, QIANG GAO, SHI CHENG, GUANGHUI |
author_facet | LIN, YUANQIANG MA, QINGJIE LIN, SHAN ZHOU, HONGYAN WEN, QIANG GAO, SHI CHENG, GUANGHUI |
author_sort | LIN, YUANQIANG |
collection | PubMed |
description | The aim of the present study was to investigate the inhibitory effects of (90)Sr-(90)Y β-irradiation in a rat model of alkali burn-induced corneal neovascularization (CNV). Alkali burn-induced CNV was induced in the right eyes of 30 female Wistar rats, which were randomly divided into the following three groups (n=10/group): i) The alkali burn control group, which received a balanced salt solution treatment; ii) group 1, which received treatment with angiogenesis inhibitors; and iii) group 2, which received (90)Sr-(90)Y β-irradiation treatment. A further 10 female Wistar rats comprised a blank control group and received only balanced salt solution. Digital photographs of the corneas were acquired and the area of NV was calculated. In addition, the expression levels of matrix metalloproteinase (MMP)-9, vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-1 and VEGFR-2 in alkali-burned rat corneas were determined using western blot analysis. The results suggested that the number of new vessels and the area of CNV were significantly decreased in groups 1 and 2, as compared with the alkali burn group at each time point (P<0.05). In addition, the number of inflammatory cells and the degree of edema were decreased in groups 1 and 2, as compared with the alkali burn group, with group 2 exhibiting the most marked reduction. Western blot analysis demonstrated that the expression levels of MMP-9, VEGF, VEGFR-1 and VEGFR-2 were significantly decreased in groups 1 and 2, as compared with the alkali burn control group, with group 2 exhibiting the most significant reduction (P<0.05). The results of the present study suggested that (90)Sr-(90)Y β-irradiation and angiogenesis inhibitor treatments were able to inhibit alkali burn-induced CNV, although (90)Sr-(90)Y β-irradiation may be more effective. |
format | Online Article Text |
id | pubmed-4734155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-47341552016-02-18 Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats LIN, YUANQIANG MA, QINGJIE LIN, SHAN ZHOU, HONGYAN WEN, QIANG GAO, SHI CHENG, GUANGHUI Exp Ther Med Articles The aim of the present study was to investigate the inhibitory effects of (90)Sr-(90)Y β-irradiation in a rat model of alkali burn-induced corneal neovascularization (CNV). Alkali burn-induced CNV was induced in the right eyes of 30 female Wistar rats, which were randomly divided into the following three groups (n=10/group): i) The alkali burn control group, which received a balanced salt solution treatment; ii) group 1, which received treatment with angiogenesis inhibitors; and iii) group 2, which received (90)Sr-(90)Y β-irradiation treatment. A further 10 female Wistar rats comprised a blank control group and received only balanced salt solution. Digital photographs of the corneas were acquired and the area of NV was calculated. In addition, the expression levels of matrix metalloproteinase (MMP)-9, vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-1 and VEGFR-2 in alkali-burned rat corneas were determined using western blot analysis. The results suggested that the number of new vessels and the area of CNV were significantly decreased in groups 1 and 2, as compared with the alkali burn group at each time point (P<0.05). In addition, the number of inflammatory cells and the degree of edema were decreased in groups 1 and 2, as compared with the alkali burn group, with group 2 exhibiting the most marked reduction. Western blot analysis demonstrated that the expression levels of MMP-9, VEGF, VEGFR-1 and VEGFR-2 were significantly decreased in groups 1 and 2, as compared with the alkali burn control group, with group 2 exhibiting the most significant reduction (P<0.05). The results of the present study suggested that (90)Sr-(90)Y β-irradiation and angiogenesis inhibitor treatments were able to inhibit alkali burn-induced CNV, although (90)Sr-(90)Y β-irradiation may be more effective. D.A. Spandidos 2016-02 2015-12-02 /pmc/articles/PMC4734155/ /pubmed/26893623 http://dx.doi.org/10.3892/etm.2015.2907 Text en Copyright: © Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles LIN, YUANQIANG MA, QINGJIE LIN, SHAN ZHOU, HONGYAN WEN, QIANG GAO, SHI CHENG, GUANGHUI Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats |
title | Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats |
title_full | Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats |
title_fullStr | Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats |
title_full_unstemmed | Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats |
title_short | Inhibitory effects of (90)Sr/(90)Y β-irradiation on alkali burn-induced corneal neovascularization in rats |
title_sort | inhibitory effects of (90)sr/(90)y β-irradiation on alkali burn-induced corneal neovascularization in rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734155/ https://www.ncbi.nlm.nih.gov/pubmed/26893623 http://dx.doi.org/10.3892/etm.2015.2907 |
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