Cargando…
Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress
Fusarium oxysporum f.sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734173/ https://www.ncbi.nlm.nih.gov/pubmed/26870056 http://dx.doi.org/10.3389/fpls.2016.00031 |
_version_ | 1782412891229519872 |
---|---|
author | Pu, Zijing Ino, Yoko Kimura, Yayoi Tago, Asumi Shimizu, Motoki Natsume, Satoshi Sano, Yoshitaka Fujimoto, Ryo Kaneko, Kentaro Shea, Daniel J. Fukai, Eigo Fuji, Shin-Ichi Hirano, Hisashi Okazaki, Keiichi |
author_facet | Pu, Zijing Ino, Yoko Kimura, Yayoi Tago, Asumi Shimizu, Motoki Natsume, Satoshi Sano, Yoshitaka Fujimoto, Ryo Kaneko, Kentaro Shea, Daniel J. Fukai, Eigo Fuji, Shin-Ichi Hirano, Hisashi Okazaki, Keiichi |
author_sort | Pu, Zijing |
collection | PubMed |
description | Fusarium oxysporum f.sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change > = 2-fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and 10 of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions. |
format | Online Article Text |
id | pubmed-4734173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47341732016-02-11 Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress Pu, Zijing Ino, Yoko Kimura, Yayoi Tago, Asumi Shimizu, Motoki Natsume, Satoshi Sano, Yoshitaka Fujimoto, Ryo Kaneko, Kentaro Shea, Daniel J. Fukai, Eigo Fuji, Shin-Ichi Hirano, Hisashi Okazaki, Keiichi Front Plant Sci Plant Science Fusarium oxysporum f.sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change > = 2-fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and 10 of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions. Frontiers Media S.A. 2016-02-01 /pmc/articles/PMC4734173/ /pubmed/26870056 http://dx.doi.org/10.3389/fpls.2016.00031 Text en Copyright © 2016 Pu, Ino, Kimura, Tago, Shimizu, Natsume, Sano, Fujimoto, Kaneko, Shea, Fukai, Fuji, Hirano and Okazaki. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Pu, Zijing Ino, Yoko Kimura, Yayoi Tago, Asumi Shimizu, Motoki Natsume, Satoshi Sano, Yoshitaka Fujimoto, Ryo Kaneko, Kentaro Shea, Daniel J. Fukai, Eigo Fuji, Shin-Ichi Hirano, Hisashi Okazaki, Keiichi Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress |
title | Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress |
title_full | Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress |
title_fullStr | Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress |
title_full_unstemmed | Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress |
title_short | Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress |
title_sort | changes in the proteome of xylem sap in brassica oleracea in response to fusarium oxysporum stress |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734173/ https://www.ncbi.nlm.nih.gov/pubmed/26870056 http://dx.doi.org/10.3389/fpls.2016.00031 |
work_keys_str_mv | AT puzijing changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT inoyoko changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT kimurayayoi changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT tagoasumi changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT shimizumotoki changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT natsumesatoshi changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT sanoyoshitaka changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT fujimotoryo changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT kanekokentaro changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT sheadanielj changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT fukaieigo changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT fujishinichi changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT hiranohisashi changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress AT okazakikeiichi changesintheproteomeofxylemsapinbrassicaoleraceainresponsetofusariumoxysporumstress |