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Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses

The impact of external medium conductivity on the efficiency of the reversible permeabilisation caused by pulsed electric fields was investigated. Pulses of 12 ns, 102 ns or 100 μs were investigated. Whenever permeabilisation could be detected after the delivery of one single pulse, media of lower c...

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Autores principales: Silve, Aude, Leray, Isabelle, Poignard, Clair, Mir, Lluis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734290/
https://www.ncbi.nlm.nih.gov/pubmed/26829153
http://dx.doi.org/10.1038/srep19957
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author Silve, Aude
Leray, Isabelle
Poignard, Clair
Mir, Lluis M.
author_facet Silve, Aude
Leray, Isabelle
Poignard, Clair
Mir, Lluis M.
author_sort Silve, Aude
collection PubMed
description The impact of external medium conductivity on the efficiency of the reversible permeabilisation caused by pulsed electric fields was investigated. Pulses of 12 ns, 102 ns or 100 μs were investigated. Whenever permeabilisation could be detected after the delivery of one single pulse, media of lower conductivity induced more efficient reversible permeabilisation and thus independently of the medium composition. Effect of medium conductivity can however be hidden by some saturation effects, for example when pulses are cumulated (use of trains of 8 pulses) or when the detection method is not sensitive enough. This explains the contradicting results that can be found in the literature. The new data are complementary to those of one of our previous study in which an opposite effect of the conductivity was highlighted. It stresses that the conductivity of the medium influences the reversible permeabilization by several ways. Moreover, these results clearly indicate that electropermeabilisation does not linearly depend on the energy delivered to the cells.
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spelling pubmed-47342902016-02-05 Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses Silve, Aude Leray, Isabelle Poignard, Clair Mir, Lluis M. Sci Rep Article The impact of external medium conductivity on the efficiency of the reversible permeabilisation caused by pulsed electric fields was investigated. Pulses of 12 ns, 102 ns or 100 μs were investigated. Whenever permeabilisation could be detected after the delivery of one single pulse, media of lower conductivity induced more efficient reversible permeabilisation and thus independently of the medium composition. Effect of medium conductivity can however be hidden by some saturation effects, for example when pulses are cumulated (use of trains of 8 pulses) or when the detection method is not sensitive enough. This explains the contradicting results that can be found in the literature. The new data are complementary to those of one of our previous study in which an opposite effect of the conductivity was highlighted. It stresses that the conductivity of the medium influences the reversible permeabilization by several ways. Moreover, these results clearly indicate that electropermeabilisation does not linearly depend on the energy delivered to the cells. Nature Publishing Group 2016-02-01 /pmc/articles/PMC4734290/ /pubmed/26829153 http://dx.doi.org/10.1038/srep19957 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Silve, Aude
Leray, Isabelle
Poignard, Clair
Mir, Lluis M.
Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
title Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
title_full Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
title_fullStr Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
title_full_unstemmed Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
title_short Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
title_sort impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734290/
https://www.ncbi.nlm.nih.gov/pubmed/26829153
http://dx.doi.org/10.1038/srep19957
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