Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a n...

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Autores principales: Hoenen, Thomas, Groseth, Allison, Rosenke, Kyle, Fischer, Robert J., Hoenen, Andreas, Judson, Seth D., Martellaro, Cynthia, Falzarano, Darryl, Marzi, Andrea, Squires, R. Burke, Wollenberg, Kurt R., de Wit, Emmie, Prescott, Joseph, Safronetz, David, van Doremalen, Neeltje, Bushmaker, Trenton, Feldmann, Friederike, McNally, Kristin, Bolay, Fatorma K., Fields, Barry, Sealy, Tara, Rayfield, Mark, Nichol, Stuart T., Zoon, Kathryn C., Massaquoi, Moses, Munster, Vincent J., Feldmann, Heinz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734547/
https://www.ncbi.nlm.nih.gov/pubmed/26812583
http://dx.doi.org/10.3201/eid2202.151796
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author Hoenen, Thomas
Groseth, Allison
Rosenke, Kyle
Fischer, Robert J.
Hoenen, Andreas
Judson, Seth D.
Martellaro, Cynthia
Falzarano, Darryl
Marzi, Andrea
Squires, R. Burke
Wollenberg, Kurt R.
de Wit, Emmie
Prescott, Joseph
Safronetz, David
van Doremalen, Neeltje
Bushmaker, Trenton
Feldmann, Friederike
McNally, Kristin
Bolay, Fatorma K.
Fields, Barry
Sealy, Tara
Rayfield, Mark
Nichol, Stuart T.
Zoon, Kathryn C.
Massaquoi, Moses
Munster, Vincent J.
Feldmann, Heinz
author_facet Hoenen, Thomas
Groseth, Allison
Rosenke, Kyle
Fischer, Robert J.
Hoenen, Andreas
Judson, Seth D.
Martellaro, Cynthia
Falzarano, Darryl
Marzi, Andrea
Squires, R. Burke
Wollenberg, Kurt R.
de Wit, Emmie
Prescott, Joseph
Safronetz, David
van Doremalen, Neeltje
Bushmaker, Trenton
Feldmann, Friederike
McNally, Kristin
Bolay, Fatorma K.
Fields, Barry
Sealy, Tara
Rayfield, Mark
Nichol, Stuart T.
Zoon, Kathryn C.
Massaquoi, Moses
Munster, Vincent J.
Feldmann, Heinz
author_sort Hoenen, Thomas
collection PubMed
description Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.
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spelling pubmed-47345472016-02-11 Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool Hoenen, Thomas Groseth, Allison Rosenke, Kyle Fischer, Robert J. Hoenen, Andreas Judson, Seth D. Martellaro, Cynthia Falzarano, Darryl Marzi, Andrea Squires, R. Burke Wollenberg, Kurt R. de Wit, Emmie Prescott, Joseph Safronetz, David van Doremalen, Neeltje Bushmaker, Trenton Feldmann, Friederike McNally, Kristin Bolay, Fatorma K. Fields, Barry Sealy, Tara Rayfield, Mark Nichol, Stuart T. Zoon, Kathryn C. Massaquoi, Moses Munster, Vincent J. Feldmann, Heinz Emerg Infect Dis Dispatch Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak. Centers for Disease Control and Prevention 2016-02 /pmc/articles/PMC4734547/ /pubmed/26812583 http://dx.doi.org/10.3201/eid2202.151796 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Hoenen, Thomas
Groseth, Allison
Rosenke, Kyle
Fischer, Robert J.
Hoenen, Andreas
Judson, Seth D.
Martellaro, Cynthia
Falzarano, Darryl
Marzi, Andrea
Squires, R. Burke
Wollenberg, Kurt R.
de Wit, Emmie
Prescott, Joseph
Safronetz, David
van Doremalen, Neeltje
Bushmaker, Trenton
Feldmann, Friederike
McNally, Kristin
Bolay, Fatorma K.
Fields, Barry
Sealy, Tara
Rayfield, Mark
Nichol, Stuart T.
Zoon, Kathryn C.
Massaquoi, Moses
Munster, Vincent J.
Feldmann, Heinz
Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_full Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_fullStr Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_full_unstemmed Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_short Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool
title_sort nanopore sequencing as a rapidly deployable ebola outbreak tool
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734547/
https://www.ncbi.nlm.nih.gov/pubmed/26812583
http://dx.doi.org/10.3201/eid2202.151796
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